Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RIN60_RS03755 Genome accession   NZ_CP134165
Coordinates   784212..785210 (+) Length   332 a.a.
NCBI ID   WP_061283232.1    Uniprot ID   A0AAW9C3L8
Organism   Kluyvera cryocrescens strain 1.8.5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 779212..790210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIN60_RS03725 (RIN60_03725) - 779591..780577 (-) 987 WP_313158436.1 TRAP transporter substrate-binding protein -
  RIN60_RS03730 (RIN60_03730) hemW 780888..782024 (-) 1137 WP_061283227.1 radical SAM family heme chaperone HemW -
  RIN60_RS03735 (RIN60_03735) - 782017..782610 (-) 594 WP_313843457.1 XTP/dITP diphosphatase -
  RIN60_RS03740 (RIN60_03740) yggU 782619..782909 (-) 291 WP_061283229.1 DUF167 family protein YggU -
  RIN60_RS03745 (RIN60_03745) - 782906..783472 (-) 567 WP_313843458.1 YggT family protein -
  RIN60_RS03750 (RIN60_03750) - 783491..784195 (-) 705 WP_061283231.1 YggS family pyridoxal phosphate-dependent enzyme -
  RIN60_RS03755 (RIN60_03755) pilT 784212..785210 (+) 999 WP_061283232.1 type IV pilus twitching motility protein PilT Machinery gene
  RIN60_RS03760 (RIN60_03760) - 785726..786379 (+) 654 WP_061283233.1 LuxR C-terminal-related transcriptional regulator -
  RIN60_RS03765 (RIN60_03765) ruvX 786552..786968 (-) 417 WP_052281945.1 Holliday junction resolvase RuvX -
  RIN60_RS03770 (RIN60_03770) - 786968..787531 (-) 564 WP_313843459.1 YqgE/AlgH family protein -
  RIN60_RS03775 (RIN60_03775) gshB 787678..788625 (-) 948 WP_061283235.1 glutathione synthase -
  RIN60_RS03780 (RIN60_03780) rsmE 788638..789369 (-) 732 WP_061283236.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36300.55 Da        Isoelectric Point: 6.4979

>NTDB_id=878915 RIN60_RS03755 WP_061283232.1 784212..785210(+) (pilT) [Kluyvera cryocrescens strain 1.8.5]
MDIQEIVALSVKHNVSDLHLCSGGAVRWRRVGALEMAPFTAAEPDTLLAQWLSEEQQREWREYRQLDFSVSLESCPRLRA
SAYANSSGISLVLRLLPTRCPTLAELHTPVALLELLHEESGLILVTGATGSGKSTTLAAMVDYLNHHVSGHILTLEDPVE
FLHASAGCLISQREIGQHAPSFAQALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRDAAQAIERLVDV
FPAQEKEPVRSQLANCLTAVVAQKLQPDGQGRRVALFEMLVNTPAVGNLIREGKTHQLTGVMQTGLQSGMQTFSQSYQQR
IKAGMLRKSESA

Nucleotide


Download         Length: 999 bp        

>NTDB_id=878915 RIN60_RS03755 WP_061283232.1 784212..785210(+) (pilT) [Kluyvera cryocrescens strain 1.8.5]
ATGGATATACAAGAAATTGTGGCGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAGCGGCGGTGCGGTGCG
CTGGCGTCGGGTTGGGGCGCTGGAAATGGCGCCGTTTACGGCAGCGGAACCGGATACGCTGTTGGCGCAGTGGTTGAGCG
AAGAGCAACAGCGGGAGTGGCGGGAGTACCGGCAGCTTGATTTCTCGGTGTCTTTAGAAAGCTGCCCGCGCCTGCGTGCC
AGTGCGTATGCCAATAGTAGCGGTATTTCGCTGGTTCTACGGCTGCTGCCTACTCGCTGCCCTACGCTCGCCGAGCTGCA
TACACCGGTAGCGCTGTTGGAACTGCTACACGAAGAGAGCGGGTTAATTTTGGTCACCGGCGCGACGGGGAGCGGGAAAT
CGACTACGCTGGCGGCAATGGTGGATTATCTTAATCACCATGTGTCAGGGCATATTCTGACGCTCGAAGATCCGGTGGAA
TTTCTCCATGCCAGTGCGGGGTGTTTGATTTCTCAGCGCGAGATAGGCCAGCATGCGCCGTCTTTTGCCCAGGCGCTGCG
GGTAGCTTTGCGTCAGGATCCTGATGTGATTCTGCTCGGCGAGCTGCGCGATAGCGAAACTATTCGCCTGGCCCTCACTG
CGGCAGAGACCGGGCATCTGGTATTGGCAACCCTGCATACCCGCGATGCCGCCCAGGCAATCGAACGTTTGGTCGATGTT
TTCCCCGCGCAGGAAAAGGAGCCGGTACGCAGTCAGCTGGCAAATTGCCTGACCGCGGTGGTCGCACAAAAGCTGCAACC
CGACGGGCAAGGGCGGCGGGTGGCCTTATTTGAGATGCTGGTCAATACGCCCGCCGTGGGTAATCTCATTCGTGAAGGTA
AAACGCACCAGCTTACGGGCGTTATGCAGACCGGATTACAGTCAGGGATGCAGACTTTTAGCCAAAGCTACCAGCAACGT
ATAAAGGCAGGTATGCTGCGAAAGAGTGAGTCTGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.85

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.85

100

0.5
  pilT Neisseria meningitidis 8013

48.503

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.204

100

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.547

100

0.467
  pilT Pseudomonas stutzeri DSM 10701

44.838

100

0.458
  pilT Acinetobacter nosocomialis M2

45.21

100

0.455
  pilT Acinetobacter baumannii D1279779

45.21

100

0.455
  pilT Acinetobacter baumannii strain A118

45.21

100

0.455
  pilT Acinetobacter baylyi ADP1

45.897

99.096

0.455
  pilT Legionella pneumophila strain ERS1305867

45.619

99.699

0.455
  pilT Legionella pneumophila strain Lp02

45.619

99.699

0.455
  pilT Pseudomonas aeruginosa PAK

44.611

100

0.449
  pilU Pseudomonas stutzeri DSM 10701

41.433

96.687

0.401
  pilU Vibrio cholerae strain A1552

40.683

96.988

0.395
  pilU Acinetobacter baylyi ADP1

40.123

97.59

0.392