Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHS38_RS02450 Genome accession   NZ_CP133891
Coordinates   504977..506017 (+) Length   346 a.a.
NCBI ID   WP_085609655.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain M0904     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 499977..511017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHS38_RS02420 (RHS38_02420) - 501118..501720 (-) 603 WP_005482459.1 XTP/dITP diphosphatase -
  RHS38_RS02425 (RHS38_02425) - 501838..502269 (-) 432 WP_005461703.1 DUF4426 domain-containing protein -
  RHS38_RS02430 (RHS38_02430) yggU 502397..502687 (-) 291 WP_053316657.1 DUF167 family protein YggU -
  RHS38_RS02435 (RHS38_02435) - 502687..503244 (-) 558 WP_005482440.1 YggT family protein -
  RHS38_RS02440 (RHS38_02440) proC 503297..504115 (-) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  RHS38_RS02445 (RHS38_02445) - 504235..504948 (-) 714 WP_005461710.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHS38_RS02450 (RHS38_02450) pilT 504977..506017 (+) 1041 WP_085609655.1 type IV pilus twitching motility protein PilT Machinery gene
  RHS38_RS02455 (RHS38_02455) pilU 506048..507154 (+) 1107 WP_005461714.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHS38_RS02460 (RHS38_02460) ruvX 507215..507640 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  RHS38_RS02465 (RHS38_02465) - 507689..508252 (-) 564 WP_005461717.1 YqgE/AlgH family protein -
  RHS38_RS02470 (RHS38_02470) gshB 508366..509316 (-) 951 WP_005425823.1 glutathione synthase -
  RHS38_RS02475 (RHS38_02475) rsmE 509331..510062 (-) 732 WP_021454379.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RHS38_RS02480 (RHS38_02480) endA 510187..510882 (-) 696 WP_057619590.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38548.02 Da        Isoelectric Point: 6.5037

>NTDB_id=877376 RHS38_RS02450 WP_085609655.1 504977..506017(+) (pilT) [Vibrio parahaemolyticus strain M0904]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQDVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=877376 RHS38_RS02450 WP_085609655.1 504977..506017(+) (pilT) [Vibrio parahaemolyticus strain M0904]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGACGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTGTTCCGTACCATCCCAAGTAGCATCCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATCGCGAATGCTGAAAAAGGTTTGGTTTTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACAACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATATCCTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAGTGTTTGATCAACCAACGTGAGGTTCACCGTGATACCCATAGCTTCCAAAA
CGCGCTACGCAGCGCATTGCGTGAAGACCCGGATGTGATCCTTGTCGGTGAGATGCGTGACAAAGAAACCATCAGCCTAG
CGTTAACGGCGGCGGAAACGGGTCACCTAGTTTTCGGTACGTTGCACACCAGTTCAGCAGCAAAAACCATCGACCGTATC
ATCGATGTGTTCCCAGGTAGTGACAAAGACATGGTGCGTTCCATGTTGTCTGAATCCCTTCGTTCAGTTATCGCGCAAAA
ACTGTTGAAACGTAATGGCGGTGGTCGTATCGCTTGTCACGAAATCATGATGGCAACGCCCGCGATTCGTAACTTGATTC
GCGAAGACAAAGTCGCGCAGATGTACTCAATCATTCAAACGGGTGCCGCGCATGGAATGCAAACCATGGAACAAAATGCC
CGTCAGTTGATGGCGCAAGGTATGGTTTCGCGCGAAGAAGTCGACAGCAAGATTGGGCTTGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.536

99.711

0.873
  pilT Vibrio cholerae strain A1552

87.536

99.711

0.873
  pilT Acinetobacter baumannii D1279779

73.03

95.376

0.697
  pilT Acinetobacter baumannii strain A118

73.03

95.376

0.697
  pilT Acinetobacter nosocomialis M2

72.727

95.376

0.694
  pilT Pseudomonas aeruginosa PAK

69.733

97.399

0.679
  pilT Acinetobacter baylyi ADP1

71.212

95.376

0.679
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.399

0.676
  pilT Legionella pneumophila strain Lp02

68.58

95.665

0.656
  pilT Legionella pneumophila strain ERS1305867

68.58

95.665

0.656
  pilT Neisseria meningitidis 8013

65.465

96.243

0.63
  pilT Neisseria gonorrhoeae MS11

65.165

96.243

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.459

91.908

0.491
  pilU Vibrio cholerae strain A1552

42.216

96.532

0.408
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.532

0.396
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364