Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHM55_RS13400 Genome accession   NZ_CP133784
Coordinates   2848689..2849723 (-) Length   344 a.a.
NCBI ID   WP_322176874.1    Uniprot ID   -
Organism   Pseudomonas sp. MH9.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2843689..2854723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM55_RS13375 (RHM55_13375) - 2845042..2845599 (-) 558 WP_322176869.1 DUF3304 domain-containing protein -
  RHM55_RS13380 (RHM55_13380) - 2845584..2846105 (-) 522 WP_322176870.1 DUF3304 domain-containing protein -
  RHM55_RS13385 (RHM55_13385) - 2846102..2846938 (-) 837 WP_322176871.1 DUF4123 domain-containing protein -
  RHM55_RS13390 (RHM55_13390) - 2847244..2847663 (-) 420 WP_322176872.1 TM2 domain-containing protein -
  RHM55_RS13395 (RHM55_13395) - 2847914..2848606 (+) 693 WP_322176873.1 C40 family peptidase -
  RHM55_RS13400 (RHM55_13400) pilT 2848689..2849723 (-) 1035 WP_322176874.1 type IV pilus twitching motility protein PilT Machinery gene
  RHM55_RS13405 (RHM55_13405) - 2849779..2850465 (+) 687 WP_322176875.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHM55_RS13410 (RHM55_13410) proC 2850493..2851311 (+) 819 WP_322176876.1 pyrroline-5-carboxylate reductase -
  RHM55_RS13415 (RHM55_13415) - 2851339..2851929 (+) 591 WP_322176877.1 YggT family protein -
  RHM55_RS13420 (RHM55_13420) metX 2852032..2853171 (+) 1140 WP_322176878.1 homoserine O-succinyltransferase MetX -
  RHM55_RS13425 (RHM55_13425) metW 2853179..2853799 (+) 621 WP_322176879.1 methionine biosynthesis protein MetW -
  RHM55_RS13430 (RHM55_13430) - 2853823..2854257 (+) 435 WP_322176880.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38035.71 Da        Isoelectric Point: 6.6682

>NTDB_id=876782 RHM55_RS13400 WP_322176874.1 2848689..2849723(-) (pilT) [Pseudomonas sp. MH9.2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVQELIYDIMNDKQRKDFEEFLETDFSFEVPGIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMIDYLNSNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKSMVRSMLSESLQAVISQTLVKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMSL
KELVTRGLITREGAREKAKTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=876782 RHM55_RS13400 WP_322176874.1 2848689..2849723(-) (pilT) [Pseudomonas sp. MH9.2]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAGCAGGGCGCGTCGGACTTGCATCTCTCCGCCGGGCTGCCACCGAT
GATTCGAGTCGATGGCGATGTACGGCGGATCAACCTGCCGGCGCTGGACCACAAACAGGTTCAGGAACTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAGTTTTTGGAGACTGACTTTTCCTTCGAGGTGCCTGGTATTGCGCGG
TTCCGGGTGAACGCTTTCAACCAGAATCGGGGTGCCGGTGCGGTGTTTCGGACTATTCCGTCGAAAGTCCTGACCATGGA
CGATCTCGGCATGGGAGAAGTGTTTCGCAAGATTACCGACGTGCCGCGCGGGCTGGTATTGGTTACCGGCCCGACCGGTT
CAGGGAAGTCCACCACCCTGGCGGCGATGATCGATTACCTGAACAGTAACAAGCATCACCACATTCTGACGATTGAAGAC
CCGATCGAGTTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAAGTCCATCGAGACACGTTGGGCTTCTCTGA
GGCCTTGCGTTCGGCCTTGCGGGAAGACCCGGACATCATTCTGGTGGGTGAAATGCGTGACCTTGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGACATTTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCGAAAACCATCGACCGGGTT
GTCGATGTGTTCCCGGCGCAAGAGAAATCGATGGTGCGCTCGATGCTGTCCGAGTCGTTGCAGGCCGTTATCTCGCAAAC
GCTGGTCAAGAAAGTCGGTGGCGGGCGGGTCGCCGCACACGAAATCATGATTGGTACACCGGCGATCCGTAACCTTATTC
GCGAGGACAAGGTCGCGCAGATGTACTCATCGATCCAGACGGGAGGTTCGTTGGGCATGCAGACGCTGGACATGAGCTTG
AAAGAGTTGGTGACCAGAGGCCTGATCACCCGCGAAGGTGCTAGGGAGAAGGCCAAGACACCAGACAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.023

100

0.93
  pilT Pseudomonas aeruginosa PAK

91.57

100

0.916
  pilT Acinetobacter nosocomialis M2

82.558

100

0.826
  pilT Acinetobacter baumannii D1279779

82.558

100

0.826
  pilT Acinetobacter baumannii strain A118

82.558

100

0.826
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.739

100

0.419
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.42

100

0.395