Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHM68_RS23885 Genome accession   NZ_CP133782
Coordinates   5257902..5258936 (-) Length   344 a.a.
NCBI ID   WP_322219457.1    Uniprot ID   -
Organism   Pseudomonas sp. DC1.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5252902..5263936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM68_RS23855 (RHM68_23855) ruvX 5253121..5253558 (+) 438 WP_322219451.1 Holliday junction resolvase RuvX -
  RHM68_RS23860 (RHM68_23860) pyrR 5253649..5254155 (+) 507 WP_322219452.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  RHM68_RS23865 (RHM68_23865) - 5254183..5255202 (+) 1020 WP_322219453.1 aspartate carbamoyltransferase catalytic subunit -
  RHM68_RS23870 (RHM68_23870) - 5255199..5256470 (+) 1272 WP_322219454.1 dihydroorotase -
  RHM68_RS23875 (RHM68_23875) - 5256547..5256978 (-) 432 WP_322219455.1 TM2 domain-containing protein -
  RHM68_RS23880 (RHM68_23880) - 5257229..5257849 (+) 621 WP_322219456.1 C40 family peptidase -
  RHM68_RS23885 (RHM68_23885) pilT 5257902..5258936 (-) 1035 WP_322219457.1 type IV pilus twitching motility protein PilT Machinery gene
  RHM68_RS23890 (RHM68_23890) - 5258993..5259715 (+) 723 WP_322219458.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHM68_RS23895 (RHM68_23895) proC 5259712..5260530 (+) 819 WP_322219459.1 pyrroline-5-carboxylate reductase -
  RHM68_RS23900 (RHM68_23900) - 5260555..5261142 (+) 588 WP_322219460.1 YggT family protein -
  RHM68_RS23905 (RHM68_23905) - 5261142..5261438 (+) 297 WP_322219461.1 DUF167 domain-containing protein -
  RHM68_RS23910 (RHM68_23910) metX 5261622..5262764 (+) 1143 WP_322219462.1 homoserine O-succinyltransferase MetX -
  RHM68_RS23915 (RHM68_23915) metW 5262787..5263410 (+) 624 Protein_4719 methionine biosynthesis protein MetW -
  RHM68_RS23920 (RHM68_23920) - 5263437..5263871 (+) 435 WP_322223926.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38121.67 Da        Isoelectric Point: 6.9776

>NTDB_id=876730 RHM68_RS23885 WP_322219457.1 5257902..5258936(-) (pilT) [Pseudomonas sp. DC1.2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDYEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLNMDDLGMGDVFRKITDSPRGLVLVTGPTGSGKSTTLAAMIDYLNNNRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVTKGLVSREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=876730 RHM68_RS23885 WP_322219457.1 5257902..5258936(-) (pilT) [Pseudomonas sp. DC1.2]
ATGGATATCACTGAGTTGCTGGCTTTCAGCGCCAAACAGGGCGCGTCCGACCTGCACCTGTCTGCCGGCCTGCCACCGAT
GATTCGTGTGGACGGGGATGTGCGGCGTATCAACCTGCCGGCCCTGGACCACAAACAGGTGCATGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTACGAGAAACACCTGGAAACCGACTTTTCCTTCGAAGTGCCCGGTGTCGCGCGC
TTTCGGGTCAACGCGTTCAACCAGAATCGCGGTGCTGGCGCGGTGTTTCGTACCATTCCGTCAAAGGTCTTGAACATGGA
CGACCTGGGCATGGGCGACGTGTTCCGCAAGATTACCGATTCGCCTCGGGGCCTGGTGTTGGTAACGGGGCCTACCGGCT
CCGGCAAGTCCACGACCCTGGCGGCGATGATTGATTACCTCAACAACAATCGCCATCACCACATTCTTACGATTGAAGAC
CCGATTGAATTCGTCCACGAATCGCGTAAATGCCTGATCAATCAGCGCGAAGTTCACCGCGATACCCGCAGTTTTGCCAC
CGCCCTGCGTTCGGCCCTGCGCGAAGACCCGGATGTGATTCTGGTGGGAGAGATGAGGGATCTCGAAACCATTCGCCTGG
CATTGACCGCCGCCGAGACGGGGCATCTGGTATTCGGCACGCTGCACACCACGTCGGCGGCGAAAACCATCGATCGTGTG
GTCGACGTGTTTCCGGGTGACGAGAAGTCCATGGTGCGCTCGATGCTGTCCGAATCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGGTCAAGAAGATCGGTGGCGGTCGGATTGCGGCGCACGAAATCATGCTCGGCACCTCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGGGGGAACTTGGGGATGCAGACGTTGGACATGTGTTTG
AAGGAGTTGGTGACCAAGGGGTTGGTCAGCCGCGAGCACGCACGGGAGAAGGCGCGCTCGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Pseudomonas aeruginosa PAK

86.337

100

0.863
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

71.512

100

0.715
  pilT Legionella pneumophila strain ERS1305867

71.512

100

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

38.551

100

0.387