Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHM56_RS24620 Genome accession   NZ_CP133778
Coordinates   5600966..5601955 (+) Length   329 a.a.
NCBI ID   WP_322236933.1    Uniprot ID   -
Organism   Pseudomonas sp. CCC3.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5595966..5606955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM56_RS24590 (RHM56_24590) - 5596378..5596800 (-) 423 WP_322236922.1 DUF4426 domain-containing protein -
  RHM56_RS24595 (RHM56_24595) metW 5596826..5597446 (-) 621 WP_322236923.1 methionine biosynthesis protein MetW -
  RHM56_RS24600 (RHM56_24600) metX 5597454..5598593 (-) 1140 WP_322236925.1 homoserine O-succinyltransferase MetX -
  RHM56_RS24605 (RHM56_24605) - 5598772..5599359 (-) 588 WP_322236927.1 YggT family protein -
  RHM56_RS24610 (RHM56_24610) proC 5599371..5600189 (-) 819 WP_322236929.1 pyrroline-5-carboxylate reductase -
  RHM56_RS24615 (RHM56_24615) - 5600222..5600908 (-) 687 WP_322236931.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHM56_RS24620 (RHM56_24620) pilT 5600966..5601955 (+) 990 WP_322236933.1 type IV pilus twitching motility protein PilT Machinery gene
  RHM56_RS24625 (RHM56_24625) - 5602044..5602703 (-) 660 WP_322236937.1 C40 family peptidase -
  RHM56_RS24630 (RHM56_24630) - 5602957..5603373 (+) 417 WP_019409165.1 NINE protein -
  RHM56_RS24635 (RHM56_24635) - 5603459..5604730 (-) 1272 WP_322236939.1 dihydroorotase -
  RHM56_RS24640 (RHM56_24640) - 5604727..5605731 (-) 1005 WP_322236941.1 aspartate carbamoyltransferase catalytic subunit -
  RHM56_RS24645 (RHM56_24645) pyrR 5605744..5606262 (-) 519 WP_322236943.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  RHM56_RS24650 (RHM56_24650) ruvX 5606346..5606786 (-) 441 WP_322236947.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 35733.22 Da        Isoelectric Point: 6.4287

>NTDB_id=876681 RHM56_RS24620 WP_322236933.1 5600966..5601955(+) (pilT) [Pseudomonas sp. CCC3.1]
MDIADLLAFSVKHGASDMHLSAGVEPMLRVDGEMQRCQLPVLAPDQVRELIRSVMSDAQRTAFETDHEADFSYTMPGLGR
FRVNAFEQHRGAGAVFRSIAAQVPSIESLGLGEVFGELCRLSQGLVLVTGATGSGKTTTLAAMLDHINTTRQQHILTIED
PIEFIHTPRQCLINQREVSRDTHSVAAALRAALREDPDVIMLGEMRDLDTIRLALTAAETGHLVLATLHTASAAKTVDRI
VDVFPAEEKAMVRAMLSESLQAVISQTLVKRVGGGRIAAHEIMLATPAIRNLIRENKVAQMVSTIQTGGAVGMQTLEMSL
KRLKENGLI

Nucleotide


Download         Length: 990 bp        

>NTDB_id=876681 RHM56_RS24620 WP_322236933.1 5600966..5601955(+) (pilT) [Pseudomonas sp. CCC3.1]
ATGGACATCGCCGACCTGTTGGCGTTTAGCGTCAAACACGGGGCTTCAGACATGCACCTGTCAGCAGGTGTGGAGCCTAT
GCTGCGGGTGGATGGGGAGATGCAGCGTTGCCAACTGCCGGTGCTGGCGCCGGATCAGGTGCGCGAACTTATCCGCAGCG
TGATGAGCGACGCACAGCGCACAGCGTTTGAAACAGATCACGAAGCTGATTTTTCGTACACGATGCCGGGCTTGGGGCGG
TTTCGGGTCAATGCCTTCGAGCAGCACCGTGGCGCTGGGGCGGTGTTTCGCAGCATTGCTGCGCAAGTCCCAAGCATTGA
ATCCCTCGGCCTTGGCGAGGTGTTTGGCGAGCTCTGTCGCCTGTCACAAGGGCTGGTGCTGGTGACGGGGGCGACTGGCT
CGGGCAAGACCACCACCTTGGCGGCAATGCTTGACCACATCAACACCACCCGTCAGCAGCACATCCTGACCATTGAAGAC
CCGATTGAGTTTATTCACACGCCGCGTCAATGCTTGATCAACCAGCGTGAAGTGTCGCGTGACACCCACAGCGTAGCGGC
GGCCTTGCGTGCGGCACTGCGCGAAGACCCGGACGTGATCATGCTGGGTGAAATGCGTGACCTGGACACCATTCGCCTGG
CGCTGACAGCAGCAGAAACGGGGCACTTGGTGTTGGCGACGCTGCACACCGCGTCGGCAGCCAAAACGGTCGACCGGATC
GTGGACGTGTTTCCAGCCGAAGAAAAAGCCATGGTGCGGGCCATGTTGTCTGAATCACTGCAGGCGGTGATTTCTCAAAC
GCTGGTTAAACGCGTGGGAGGCGGTCGGATAGCCGCTCACGAAATCATGCTGGCGACGCCGGCGATTCGTAATTTGATCC
GGGAAAACAAGGTGGCGCAGATGGTTTCTACTATCCAGACCGGCGGGGCGGTGGGGATGCAGACGTTGGAGATGAGCCTG
AAACGGCTGAAAGAAAACGGCTTGATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

68.693

100

0.687
  pilT Pseudomonas stutzeri DSM 10701

68.085

100

0.681
  pilT Acinetobacter nosocomialis M2

67.477

100

0.675
  pilT Acinetobacter baumannii D1279779

67.477

100

0.675
  pilT Acinetobacter baumannii strain A118

67.477

100

0.675
  pilT Acinetobacter baylyi ADP1

67.173

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.83

100

0.638
  pilT Vibrio cholerae strain A1552

63.83

100

0.638
  pilT Legionella pneumophila strain Lp02

61.702

100

0.617
  pilT Legionella pneumophila strain ERS1305867

61.702

100

0.617
  pilT Neisseria meningitidis 8013

59.697

100

0.599
  pilT Neisseria gonorrhoeae MS11

59.394

100

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.697

100

0.498
  pilU Pseudomonas stutzeri DSM 10701

41.818

100

0.419
  pilU Vibrio cholerae strain A1552

41.667

98.48

0.41
  pilU Acinetobacter baylyi ADP1

41.615

97.872

0.407