Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHM64_RS00645 Genome accession   NZ_CP133776
Coordinates   138448..139482 (+) Length   344 a.a.
NCBI ID   WP_322167053.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133448..144482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS00615 (RHM64_00615) - 133771..134205 (-) 435 WP_322167046.1 DUF4426 domain-containing protein -
  RHM64_RS00620 (RHM64_00620) metW 134244..134864 (-) 621 WP_322167047.1 methionine biosynthesis protein MetW -
  RHM64_RS00625 (RHM64_00625) metX 134872..136011 (-) 1140 WP_322167048.1 homoserine O-succinyltransferase MetX -
  RHM64_RS00630 (RHM64_00630) - 136261..136851 (-) 591 WP_322167049.1 YggT family protein -
  RHM64_RS00635 (RHM64_00635) proC 136862..137680 (-) 819 WP_322167050.1 pyrroline-5-carboxylate reductase -
  RHM64_RS00640 (RHM64_00640) - 137706..138392 (-) 687 WP_322167052.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHM64_RS00645 (RHM64_00645) pilT 138448..139482 (+) 1035 WP_322167053.1 type IV pilus twitching motility protein PilT Machinery gene
  RHM64_RS00650 (RHM64_00650) - 139576..140349 (-) 774 WP_322167054.1 C40 family peptidase -
  RHM64_RS00655 (RHM64_00655) - 140599..141018 (+) 420 WP_322167055.1 TM2 domain-containing protein -
  RHM64_RS00660 (RHM64_00660) - 141356..142627 (-) 1272 WP_322167056.1 dihydroorotase -
  RHM64_RS00665 (RHM64_00665) - 142624..143628 (-) 1005 WP_322167057.1 aspartate carbamoyltransferase catalytic subunit -
  RHM64_RS00670 (RHM64_00670) pyrR 143651..144163 (-) 513 WP_322167058.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37904.59 Da        Isoelectric Point: 6.7747

>NTDB_id=876608 RHM64_RS00645 WP_322167053.1 138448..139482(+) (pilT) [Pseudomonas sp. AH2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDQKTVQNLIYDIMNDKQRKDFEEFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMIDYLNSTKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKSMVRSMLSESLQAVISQTLLKKVGGGRIAAHEIMIGTAAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLVNKGLVTRESAREKAKTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=876608 RHM64_RS00645 WP_322167053.1 138448..139482(+) (pilT) [Pseudomonas sp. AH2]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAGCAGGGCGCGTCAGATTTGCATTTATCCGCTGGCCTGCCACCGAT
GATTCGTGTCGACGGTGATGTACGGCGTATCAATCTGCCGGCTTTGGACCAGAAAACGGTCCAAAATTTGATTTACGACA
TCATGAACGACAAGCAGCGTAAGGATTTCGAGGAGTTTCTGGAAACAGACTTTTCCTTCGATGTGCCTGGCGTTGCGCGC
TTTCGGGTCAACGCTTTCAACCAGAATCGCGGCGCCGGTGCGGTGTTTCGGACCATCCCCTCCAAGGTCCTGAGCATGGA
CGATCTGGGCATGGGAGAAGTGTTTCGCAAGATTACCGATGTCCCGCGCGGGCTGGTATTGGTCACCGGACCGACCGGGT
CAGGGAAATCCACTACTCTCGCGGCGATGATCGATTACCTGAACAGCACCAAGCACCACCACATCCTCACCATCGAAGAC
CCTATCGAGTTCGTTCACGAATCGAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCATCGCGACACCTTGGGTTTCGCCGA
GGCGTTGCGTTCGGCGCTGCGGGAAGACCCGGACGTCATCCTCGTCGGTGAAATGCGCGACCTCGAAACCATTCGGCTGG
CATTGACTGCCGCTGAAACCGGTCACTTGGTCTTCGGCACGCTGCACACCACCTCTGCGGCGAAAACCATCGACCGTGTG
GTTGACGTGTTCCCGGCGCAAGAGAAGTCCATGGTGCGCTCGATGTTGTCCGAATCGTTGCAGGCGGTGATCTCCCAGAC
ACTGCTGAAGAAAGTCGGCGGCGGGCGGATTGCGGCTCACGAAATCATGATTGGCACGGCGGCCATTCGTAACCTGATTC
GCGAAGATAAAGTGGCGCAGATGTACTCGTCGATCCAGACCGGTGGTTCATTGGGTATGCAGACGTTGGACATGTGCCTG
AAGGATCTGGTCAACAAAGGCTTGGTGACCCGCGAAAGCGCGCGCGAAAAAGCCAAGACCCCGGACAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

92.151

100

0.922
  pilT Pseudomonas aeruginosa PAK

90.116

100

0.901
  pilT Acinetobacter nosocomialis M2

80.233

100

0.802
  pilT Acinetobacter baumannii D1279779

80.233

100

0.802
  pilT Acinetobacter baumannii strain A118

80.233

100

0.802
  pilT Acinetobacter baylyi ADP1

79.36

100

0.794
  pilT Legionella pneumophila strain Lp02

73.669

98.256

0.724
  pilT Legionella pneumophila strain ERS1305867

73.669

98.256

0.724
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.965

0.66
  pilT Vibrio cholerae strain A1552

67.359

97.965

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

38.841

100

0.39