Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   MCIT9_RS13125 Genome accession   NZ_AP024714
Coordinates   2596733..2597149 (+) Length   138 a.a.
NCBI ID   WP_317705323.1    Uniprot ID   -
Organism   Methylomarinovum caldicuralii strain IT-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2591733..2602149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCIT9_RS13110 (MIT9_P2528) rluD 2592134..2593126 (+) 993 WP_317705320.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MCIT9_RS13115 (MIT9_P2529) pgeF 2593111..2593845 (+) 735 WP_317705321.1 peptidoglycan editing factor PgeF -
  MCIT9_RS13120 (MIT9_P2530) clpB 2593925..2596507 (+) 2583 WP_317705322.1 ATP-dependent chaperone ClpB -
  MCIT9_RS13125 pilA2 2596733..2597149 (+) 417 WP_317705323.1 pilin Machinery gene
  MCIT9_RS13130 (MIT9_P2531) - 2597140..2599068 (+) 1929 WP_317705324.1 hypothetical protein -
  MCIT9_RS13135 - 2599087..2599257 (-) 171 WP_317705325.1 hypothetical protein -
  MCIT9_RS13140 (MIT9_P2533) - 2599820..2600905 (-) 1086 WP_317705326.1 ribonucleotide-diphosphate reductase subunit beta -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14292.29 Da        Isoelectric Point: 8.4579

>NTDB_id=87655 MCIT9_RS13125 WP_317705323.1 2596733..2597149(+) (pilA2) [Methylomarinovum caldicuralii strain IT-9]
MMQQRNQQGFTLIELMIVVAIIGILAAIAIPAYQDYTKKAKAAELLNAAGPAKAAVSEFIVANNSLPSNATQAGFSTVST
DYVSSVSWDSSNNRIAVVGKGDLSGLDIYLAPTLSGGAVKWTCSSSGTNAKYAPGSCK

Nucleotide


Download         Length: 417 bp        

>NTDB_id=87655 MCIT9_RS13125 WP_317705323.1 2596733..2597149(+) (pilA2) [Methylomarinovum caldicuralii strain IT-9]
ATGATGCAACAACGCAACCAACAGGGTTTCACGCTCATCGAACTGATGATCGTGGTGGCGATCATCGGGATTTTGGCGGC
GATCGCGATTCCGGCGTATCAGGACTATACAAAGAAAGCCAAAGCGGCTGAGCTTTTGAATGCAGCTGGGCCGGCAAAAG
CTGCCGTTTCTGAATTTATTGTCGCCAACAATTCATTGCCAAGCAATGCAACTCAAGCTGGTTTCTCCACGGTATCCACA
GATTATGTGAGCAGTGTTTCTTGGGATTCCTCTAATAATCGTATTGCAGTGGTTGGTAAAGGTGATCTTTCCGGTTTAGA
CATTTATCTGGCTCCCACCTTAAGCGGCGGAGCGGTGAAATGGACGTGTAGCTCCAGTGGAACGAATGCCAAATACGCCC
CGGGCTCATGCAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(39-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.63

97.826

0.486
  pilA2 Legionella pneumophila strain ERS1305867

49.63

97.826

0.486
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.87

100

0.478
  pilA Ralstonia pseudosolanacearum GMI1000

39.521

100

0.478
  pilA Haemophilus influenzae 86-028NP

45.652

100

0.457
  comP Acinetobacter baylyi ADP1

43.056

100

0.449
  pilA Haemophilus influenzae Rd KW20

43.796

99.275

0.435
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.549

100

0.428
  pilE Neisseria gonorrhoeae MS11

36.129

100

0.406
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.399
  pilA/pilA1 Eikenella corrodens VA1

35.065

100

0.391
  pilA Vibrio cholerae C6706

35.526

100

0.391
  pilA Vibrio cholerae strain A1552

35.526

100

0.391
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.526

100

0.391
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.971

98.551

0.384
  pilA Glaesserella parasuis strain SC1401

37.778

97.826

0.37

Multiple sequence alignment