Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG903_RS11045 Genome accession   NZ_CP133759
Coordinates   2215377..2215577 (+) Length   66 a.a.
NCBI ID   WP_028989787.1    Uniprot ID   -
Organism   Thermithiobacillus tepidarius DSM 3134     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2210377..2220577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG903_RS11020 (RG903_10995) lspA 2210684..2211142 (+) 459 WP_028989784.1 signal peptidase II -
  RG903_RS11025 (RG903_11000) - 2211212..2212180 (+) 969 WP_051181187.1 MBL fold metallo-hydrolase -
  RG903_RS11030 (RG903_11005) - 2212676..2214076 (-) 1401 WP_028989785.1 phosphomannomutase/phosphoglucomutase -
  RG903_RS11035 (RG903_11010) - 2214306..2214815 (-) 510 WP_155989778.1 type IV pilin protein -
  RG903_RS11040 (RG903_11015) - 2214951..2215325 (+) 375 WP_155989779.1 hypothetical protein -
  RG903_RS11045 pilA 2215377..2215577 (+) 201 WP_028989787.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  RG903_RS11050 (RG903_11025) - 2215590..2215964 (+) 375 WP_028989788.1 hypothetical protein -
  RG903_RS11055 (RG903_11030) - 2216060..2216779 (+) 720 WP_028989789.1 ABC transporter permease subunit -
  RG903_RS11060 (RG903_11035) - 2216868..2217704 (+) 837 WP_051181192.1 hypothetical protein -
  RG903_RS11065 (RG903_11040) - 2217724..2218671 (+) 948 WP_038018944.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 66 a.a.        Molecular weight: 7342.69 Da        Isoelectric Point: 10.6777

>NTDB_id=876403 RG903_RS11045 WP_028989787.1 2215377..2215577(+) (pilA) [Thermithiobacillus tepidarius DSM 3134]
MMKLRSAKARKEQGFTLIELMIVVAIIGILAAIAIPQFSQYRERAFDKAAQSDAKNFLTSTIANIQ

Nucleotide


Download         Length: 201 bp        

>NTDB_id=876403 RG903_RS11045 WP_028989787.1 2215377..2215577(+) (pilA) [Thermithiobacillus tepidarius DSM 3134]
ATGATGAAGCTACGCAGCGCCAAGGCACGCAAGGAGCAGGGTTTTACCCTGATCGAACTCATGATCGTGGTGGCCATCAT
CGGCATCCTGGCCGCCATCGCCATTCCGCAGTTCAGCCAGTATCGTGAGCGGGCGTTCGACAAGGCGGCCCAGAGCGATG
CCAAGAACTTCCTGACCTCTACCATCGCAAATATACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.704

100

0.545
  pilA Vibrio campbellii strain DS40M4

58.491

80.303

0.47
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.902

77.273

0.424
  comF Acinetobacter baylyi ADP1

61.364

66.667

0.409
  pilE Acinetobacter baumannii D1279779

57.778

68.182

0.394
  pilA Vibrio parahaemolyticus RIMD 2210633

61.905

63.636

0.394
  pilA Haemophilus influenzae Rd KW20

59.091

66.667

0.394
  pilA Haemophilus influenzae 86-028NP

59.091

66.667

0.394
  pilA/pilAI Pseudomonas stutzeri DSM 10701

68.421

57.576

0.394
  pilA Pseudomonas aeruginosa PAK

72.222

54.545

0.394
  pilA Acinetobacter baumannii strain A118

83.333

45.455

0.379
  comP Acinetobacter baylyi ADP1

73.529

51.515

0.379
  pilA Vibrio cholerae C6706

57.143

63.636

0.364
  pilA Vibrio cholerae strain A1552

57.143

63.636

0.364
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

57.143

63.636

0.364
  pilA/pilA1 Synechocystis sp. PCC 6803

52.174

69.697

0.364
  pilA/pilA1 Eikenella corrodens VA1

70.588

51.515

0.364
  pilY2 Acinetobacter baumannii D1279779

54.545

66.667

0.364