NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	RF679_RS05055	Genome accession	NZ_CP133720
Coordinates	1226653..1227069 (-)	Length	138 a.a.
NCBI ID	WP_309483126.1	Uniprot ID	-
Organism	Undibacterium cyanobacteriorum strain 20NA77.5
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1221653..1232069

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RF679_RS05030 (RF679_05030)	sucD	1222813..1223694 (+)	882	WP_212683648.1	succinate--CoA ligase subunit alpha	-
RF679_RS05035 (RF679_05035)	-	1223827..1224555 (+)	729	WP_309483122.1	TerC family protein	-
RF679_RS05040 (RF679_05040)	-	1224582..1225388 (+)	807	WP_309483123.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
RF679_RS05045 (RF679_05045)	-	1225435..1226025 (+)	591	WP_309483124.1	FHA domain-containing protein	-
RF679_RS05050 (RF679_05050)	pilA2	1226126..1226542 (-)	417	WP_309483125.1	pilin	Machinery gene
RF679_RS05055 (RF679_05055)	pilA2	1226653..1227069 (-)	417	WP_309483126.1	pilin	Machinery gene
RF679_RS05060 (RF679_05060)	tfpZ	1227464..1228228 (+)	765	WP_309483127.1	TfpX/TfpZ family type IV pilin accessory protein	-
RF679_RS05065 (RF679_05065)	-	1228465..1230192 (+)	1728	WP_309483128.1	Wzy polymerase domain-containing protein	-
RF679_RS05070 (RF679_05070)	-	1230321..1230614 (+)	294	WP_309483129.1	hypothetical protein	-

Sequence

Protein

Download Length: 138 a.a. Molecular weight: 14369.53 Da Isoelectric Point: 8.9684

                                >NTDB_id=876093 RF679_RS05055 WP_309483126.1 1226653..1227069(-) (pilA2) [Undibacterium cyanobacteriorum strain 20NA77.5]

MKNVSKKVQQGFTLIELMIVVAIIGILAAVALPAYKDYTVRAKVTEVVLAASAGKDAVAEHYNYKGTLPTTAELNLGTQA

SQYVSSVGWSGTVLTATATGETKINGSTITLTPSASGDVLNWVCSGTIAAQYRPASCK

Nucleotide

Download Length: 417 bp

                                >NTDB_id=876093 RF679_RS05055 WP_309483126.1 1226653..1227069(-) (pilA2) [Undibacterium cyanobacteriorum strain 20NA77.5]

ATGAAAAATGTATCTAAAAAAGTTCAACAAGGTTTTACTTTGATCGAATTGATGATCGTTGTGGCGATTATCGGTATTTT

GGCTGCAGTTGCTTTGCCAGCATACAAAGACTACACAGTTCGTGCGAAAGTTACTGAAGTTGTATTGGCAGCATCTGCTG

GTAAAGATGCAGTTGCAGAGCACTACAACTACAAAGGCACATTGCCAACAACAGCTGAACTCAACCTCGGCACACAAGCA

TCACAATACGTTTCCTCTGTTGGCTGGAGTGGTACAGTTCTGACAGCAACTGCAACTGGCGAAACAAAGATCAACGGTAG

CACAATCACTTTGACACCATCCGCTAGCGGCGACGTGTTGAATTGGGTTTGCTCTGGCACGATCGCTGCTCAGTATCGTC

CAGCAAGCTGCAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)

Pilin (bacterial filament) (Pilin)

(40-138)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.968	100	0.514
pilA2	Legionella pneumophila strain ERS1305867	48.951	100	0.507
pilA2	Legionella pneumophila str. Paris	48.951	100	0.507
pilA	Ralstonia pseudosolanacearum GMI1000	40.361	100	0.486
comP	Acinetobacter baylyi ADP1	43.448	100	0.457
pilA/pilA1	Eikenella corrodens VA1	40.523	100	0.449
pilA/pilAII	Pseudomonas stutzeri DSM 10701	38.411	100	0.42
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40.288	100	0.406
pilE	Neisseria gonorrhoeae strain FA1090	45.161	89.855	0.406
pilA	Pseudomonas aeruginosa PAK	36.242	100	0.391
pilE	Neisseria gonorrhoeae MS11	42.857	91.304	0.391
pilA	Vibrio parahaemolyticus RIMD 2210633	38.76	93.478	0.362
pilA	Vibrio cholerae C6706	33.557	100	0.362
pilA	Vibrio cholerae strain A1552	33.557	100	0.362
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	33.557	100	0.362

Detailed information NTG0876093