Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   RF679_RS05050 Genome accession   NZ_CP133720
Coordinates   1226126..1226542 (-) Length   138 a.a.
NCBI ID   WP_309483125.1    Uniprot ID   -
Organism   Undibacterium cyanobacteriorum strain 20NA77.5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1221126..1231542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RF679_RS05025 (RF679_05025) sucC 1221628..1222794 (+) 1167 WP_373921738.1 ADP-forming succinate--CoA ligase subunit beta -
  RF679_RS05030 (RF679_05030) sucD 1222813..1223694 (+) 882 WP_212683648.1 succinate--CoA ligase subunit alpha -
  RF679_RS05035 (RF679_05035) - 1223827..1224555 (+) 729 WP_309483122.1 TerC family protein -
  RF679_RS05040 (RF679_05040) - 1224582..1225388 (+) 807 WP_309483123.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  RF679_RS05045 (RF679_05045) - 1225435..1226025 (+) 591 WP_309483124.1 FHA domain-containing protein -
  RF679_RS05050 (RF679_05050) pilA2 1226126..1226542 (-) 417 WP_309483125.1 pilin Machinery gene
  RF679_RS05055 (RF679_05055) pilA2 1226653..1227069 (-) 417 WP_309483126.1 pilin Machinery gene
  RF679_RS05060 (RF679_05060) tfpZ 1227464..1228228 (+) 765 WP_309483127.1 TfpX/TfpZ family type IV pilin accessory protein -
  RF679_RS05065 (RF679_05065) - 1228465..1230192 (+) 1728 WP_309483128.1 Wzy polymerase domain-containing protein -
  RF679_RS05070 (RF679_05070) - 1230321..1230614 (+) 294 WP_309483129.1 hypothetical protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14480.82 Da        Isoelectric Point: 9.5021

>NTDB_id=876092 RF679_RS05050 WP_309483125.1 1226126..1226542(-) (pilA2) [Undibacterium cyanobacteriorum strain 20NA77.5]
MHITTRNFQKGFTLIELMIVVAIIGILAAVALPAYKDYTVRAKVTEVMLAASAGKDSVVEHFVVKGTLPTTAQIALATQS
TKYVSSVGWSGTVLTATATGETKINGSTITLTPSVTGDVLNWTCSGTIAAQYRPASCK

Nucleotide


Download         Length: 417 bp        

>NTDB_id=876092 RF679_RS05050 WP_309483125.1 1226126..1226542(-) (pilA2) [Undibacterium cyanobacteriorum strain 20NA77.5]
ATGCATATCACAACAAGAAACTTTCAAAAGGGTTTTACTTTAATTGAGTTGATGATTGTTGTTGCGATCATCGGGATCTT
GGCGGCAGTCGCATTGCCAGCCTACAAAGACTACACAGTGCGCGCCAAAGTAACCGAAGTCATGTTGGCTGCAAGTGCTG
GAAAAGATTCGGTGGTCGAACACTTTGTAGTAAAAGGTACGCTACCGACAACTGCACAAATCGCTTTAGCGACACAATCT
ACTAAATATGTAAGCAGCGTAGGCTGGAGCGGAACAGTACTAACGGCGACCGCAACCGGTGAGACTAAGATTAACGGCAG
TACAATCACATTGACACCAAGCGTTACTGGCGATGTTTTGAATTGGACTTGCTCCGGCACTATCGCAGCACAGTATCGTC
CAGCCAGCTGCAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.572

100

0.536
  pilA2 Legionella pneumophila strain ERS1305867

50

100

0.522
  pilA2 Legionella pneumophila str. Paris

50

100

0.522
  pilA Ralstonia pseudosolanacearum GMI1000

40.351

100

0.5
  comP Acinetobacter baylyi ADP1

43.537

100

0.464
  pilA/pilA1 Eikenella corrodens VA1

39.216

100

0.435
  pilE Neisseria gonorrhoeae strain FA1090

46.774

89.855

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.007

100

0.413
  pilE Neisseria gonorrhoeae MS11

44.8

90.58

0.406
  pilA Pseudomonas aeruginosa PAK

37.415

100

0.399
  pilA Acinetobacter baumannii strain A118

38.571

100

0.391
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.461

100

0.362