Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RFN81_RS14120 Genome accession   NZ_CP133656
Coordinates   3081771..3082766 (-) Length   331 a.a.
NCBI ID   WP_264496425.1    Uniprot ID   -
Organism   Pectobacterium cacticida strain CFBP3628     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3076771..3087766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN81_RS14095 (RFN81_14095) - 3077445..3078185 (-) 741 WP_264496420.1 amino acid ABC transporter ATP-binding protein -
  RFN81_RS14100 (RFN81_14100) - 3078182..3078907 (-) 726 WP_264496421.1 amino acid ABC transporter permease -
  RFN81_RS14105 (RFN81_14105) - 3078936..3079748 (-) 813 WP_264496422.1 ABC transporter substrate-binding protein -
  RFN81_RS14110 (RFN81_14110) - 3079939..3080835 (+) 897 WP_264496423.1 GntR family transcriptional regulator -
  RFN81_RS14115 (RFN81_14115) - 3080887..3081672 (+) 786 WP_264496424.1 SDR family oxidoreductase -
  RFN81_RS14120 (RFN81_14120) pilT 3081771..3082766 (-) 996 WP_264496425.1 type IV pilus twitching motility protein PilT Machinery gene
  RFN81_RS14125 (RFN81_14125) - 3082788..3083504 (+) 717 WP_264496426.1 YggS family pyridoxal phosphate-dependent enzyme -
  RFN81_RS14130 (RFN81_14130) proC 3083584..3084405 (+) 822 WP_264496427.1 pyrroline-5-carboxylate reductase -
  RFN81_RS14135 (RFN81_14135) - 3084451..3085005 (+) 555 WP_264496428.1 YggT family protein -
  RFN81_RS14140 (RFN81_14140) yggU 3085002..3085292 (+) 291 WP_264496429.1 DUF167 family protein YggU -
  RFN81_RS14145 (RFN81_14145) - 3085310..3085903 (+) 594 WP_264496430.1 XTP/dITP diphosphatase -
  RFN81_RS14150 (RFN81_14150) hemW 3085896..3087038 (+) 1143 WP_264496431.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 36357.65 Da        Isoelectric Point: 7.0924

>NTDB_id=875627 RFN81_RS14120 WP_264496425.1 3081771..3082766(-) (pilT) [Pectobacterium cacticida strain CFBP3628]
MELDEWVARSVKHNASDLHLCSGHPPVIRVDGRLQPENTLPRLTSEQVTQWCHAWLEPTQREQLRQSGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDALHAPRVLSSLLEKPNGLILITGATGSGKSTTLAAMIGALNDSHDRHVITLE
DPIEFMHESRRCLIQQREMGTHSVSFAQALRAALREDPDVILLGELRDKETIRLALTAAETGHLVLSTLHTRSAAQAIDR
LIDVFPGEEKGYVRSQLASGLQAVVTQKLLPMAQGGRIALYEVLTTTVAVSNLIREGKTHQLPALIQTGAAAGMQTFEQS
YQQRCQCGLLP

Nucleotide


Download         Length: 996 bp        

>NTDB_id=875627 RFN81_RS14120 WP_264496425.1 3081771..3082766(-) (pilT) [Pectobacterium cacticida strain CFBP3628]
ATGGAGTTGGATGAATGGGTGGCCCGTAGTGTAAAGCATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCAGT
GATACGTGTCGATGGGCGGCTACAGCCGGAAAATACCTTACCACGTTTAACGTCGGAACAGGTGACACAATGGTGTCACG
CCTGGCTCGAACCGACGCAGAGGGAACAACTGCGGCAGTCTGGTCAGGTTGATGGCGCACTGATGCTGCCCGATGGGCAA
CGGTTGCGGGTGAATGTATTTCGCCAGCGGGAAGGGCTGTCCGCGGCGCTGCGCATCATTCCGTCCGTTCAACCGTCACT
GGATGCGCTGCATGCGCCACGGGTTCTGTCATCGCTATTGGAGAAACCGAACGGCTTGATTTTGATTACCGGTGCGACGG
GGAGTGGTAAATCCACAACGCTGGCGGCCATGATCGGAGCGTTAAACGACAGTCACGATCGCCATGTGATCACGCTGGAG
GATCCGATCGAGTTTATGCATGAGAGTCGACGCTGCCTGATTCAGCAGCGCGAGATGGGTACGCACAGCGTATCGTTTGC
ACAGGCGCTACGGGCGGCACTAAGGGAAGATCCTGATGTCATTCTGTTAGGTGAACTGCGTGATAAGGAAACGATCCGGT
TAGCATTAACGGCGGCGGAGACGGGCCACTTAGTGCTATCGACGCTGCATACCCGCAGCGCAGCGCAGGCGATCGACCGC
CTGATTGACGTTTTCCCCGGTGAAGAAAAAGGCTATGTTCGCAGCCAGTTGGCCTCCGGCTTACAGGCAGTGGTCACGCA
AAAACTCTTGCCGATGGCGCAGGGCGGCCGAATCGCACTTTATGAAGTGCTGACGACAACGGTGGCCGTCAGCAACTTGA
TTCGAGAGGGGAAAACGCATCAACTTCCCGCCCTGATTCAGACCGGCGCGGCTGCGGGCATGCAAACGTTCGAACAGAGC
TACCAGCAACGGTGCCAGTGTGGGCTATTACCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

99.698

0.52
  pilT Vibrio cholerae strain A1552

52.121

99.698

0.52
  pilT Neisseria meningitidis 8013

50.453

100

0.505
  pilT Pseudomonas stutzeri DSM 10701

50.303

99.698

0.502
  pilT Neisseria gonorrhoeae MS11

50.151

100

0.502
  pilT Acinetobacter baylyi ADP1

49.697

99.698

0.495
  pilT Legionella pneumophila strain Lp02

50.784

96.375

0.489
  pilT Legionella pneumophila strain ERS1305867

50.784

96.375

0.489
  pilT Pseudomonas aeruginosa PAK

49.091

99.698

0.489
  pilT Acinetobacter nosocomialis M2

48.788

99.698

0.486
  pilT Acinetobacter baumannii D1279779

48.788

99.698

0.486
  pilT Acinetobacter baumannii strain A118

48.788

99.698

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.887

96.375

0.423
  pilU Pseudomonas stutzeri DSM 10701

40.498

96.979

0.393
  pilU Vibrio cholerae strain A1552

40.123

97.885

0.393
  pilU Acinetobacter baylyi ADP1

37.578

97.281

0.366