Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RFB14_RS20565 Genome accession   NZ_CP133587
Coordinates   4255265..4256308 (-) Length   347 a.a.
NCBI ID   WP_122958348.1    Uniprot ID   -
Organism   Brevibacillus borstelensis strain NEB264     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4250265..4261308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFB14_RS20540 (RFB14_20540) - 4250484..4251131 (-) 648 WP_141334223.1 PilN domain-containing protein -
  RFB14_RS20545 (RFB14_20545) pilM 4251137..4252165 (-) 1029 WP_003392157.1 type IV pilus biogenesis protein PilM -
  RFB14_RS20550 (RFB14_20550) - 4252413..4253171 (-) 759 WP_122958350.1 A24 family peptidase -
  RFB14_RS20555 (RFB14_20555) - 4253576..4254025 (-) 450 WP_024985514.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RFB14_RS20560 (RFB14_20560) - 4254056..4255264 (-) 1209 WP_122958349.1 type II secretion system F family protein -
  RFB14_RS20565 (RFB14_20565) pilT 4255265..4256308 (-) 1044 WP_122958348.1 type IV pilus twitching motility protein PilT Machinery gene
  RFB14_RS20570 (RFB14_20570) - 4256342..4257997 (-) 1656 WP_024985513.1 GspE/PulE family protein -
  RFB14_RS20575 (RFB14_20575) - 4258003..4259667 (-) 1665 WP_141334225.1 VanW family protein -
  RFB14_RS20580 (RFB14_20580) - 4259676..4260869 (-) 1194 WP_171506705.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38450.48 Da        Isoelectric Point: 7.3933

>NTDB_id=875186 RFB14_RS20565 WP_122958348.1 4255265..4256308(-) (pilT) [Brevibacillus borstelensis strain NEB264]
MAGVEIKKLLRYAFEKKASDLHVTVGTSPVFRIDGELRRLDISPLAPSDTEEMAKELIPQHLYPSFLEKGELDFSYGLPG
ISRFRVNAYHQRGCIGLVARVIPTGIPSLEELGLPEVIRKLCHKPQGLLLVTGPTGSGKSTTLAAMIDYMNRTMRKHIVT
LEDPIEYLHKHQLSMVNQREIGFDTKDFASGLRASLRQDPDVILVGEMRDLETISTAVTAAETGHLVLATLHTTDAPKTI
DRIIDVFPGDKQSQIRMQLSSVLVGIVSQRLFPKVGGGRAVATEILVHTHAVANLIRSEKNHQIKSLMQTGRELGMHMME
SSVKELVDKGQITPEAAQHYLNERALE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=875186 RFB14_RS20565 WP_122958348.1 4255265..4256308(-) (pilT) [Brevibacillus borstelensis strain NEB264]
ATGGCTGGCGTCGAAATCAAAAAGCTGCTCAGATACGCTTTTGAAAAAAAGGCATCGGACCTGCATGTGACAGTAGGCAC
CTCTCCCGTATTCCGAATTGACGGGGAACTGCGCCGGCTCGACATTTCTCCGCTTGCGCCAAGCGACACCGAGGAAATGG
CCAAGGAGCTGATCCCGCAGCATTTGTATCCGTCCTTTCTGGAAAAGGGAGAATTGGATTTCTCCTACGGACTGCCGGGC
ATCTCGCGTTTCCGCGTCAATGCGTACCACCAGCGCGGGTGCATCGGGCTTGTCGCCCGGGTCATTCCGACAGGCATCCC
GTCACTGGAAGAGCTGGGACTGCCGGAGGTAATCCGGAAGCTTTGCCACAAGCCCCAAGGGTTACTGCTCGTTACAGGGC
CGACGGGAAGCGGCAAGTCGACTACATTGGCGGCGATGATCGACTACATGAACAGGACGATGAGAAAGCATATCGTTACG
CTGGAAGACCCGATCGAGTATTTGCACAAGCATCAGCTCTCCATGGTCAATCAGCGGGAGATCGGCTTTGATACCAAAGA
TTTTGCCAGCGGACTGCGGGCTTCGCTGCGGCAGGACCCTGACGTCATATTGGTCGGCGAGATGCGGGATCTGGAGACGA
TCTCGACAGCAGTCACAGCGGCGGAGACGGGACATTTGGTGCTCGCGACGCTGCATACAACCGATGCGCCCAAGACCATC
GACCGGATTATCGATGTCTTTCCGGGCGACAAGCAATCCCAGATTCGGATGCAGTTGTCTTCCGTTCTGGTCGGGATCGT
CTCGCAGCGCCTGTTTCCGAAGGTCGGGGGCGGCCGGGCAGTCGCCACTGAGATTCTCGTTCATACACATGCTGTCGCCA
ACCTGATACGCAGCGAGAAGAATCATCAAATCAAAAGCTTGATGCAGACGGGACGCGAACTGGGAATGCACATGATGGAA
AGCTCCGTCAAAGAGCTAGTGGACAAGGGACAGATTACTCCCGAAGCCGCGCAACACTATTTGAATGAAAGGGCGCTAGA
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.298

96.83

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.298

96.83

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.751

95.965

0.487
  pilT Pseudomonas aeruginosa PAK

49.552

96.542

0.478
  pilT Pseudomonas stutzeri DSM 10701

49.552

96.542

0.478
  pilT Acinetobacter baylyi ADP1

48.657

96.542

0.47
  pilT Neisseria gonorrhoeae MS11

48.214

96.83

0.467
  pilT Neisseria meningitidis 8013

48.214

96.83

0.467
  pilT Acinetobacter baumannii strain A118

48.06

96.542

0.464
  pilT Acinetobacter baumannii D1279779

48.06

96.542

0.464
  pilT Acinetobacter nosocomialis M2

48.06

96.542

0.464
  pilT Legionella pneumophila strain Lp02

46.939

98.847

0.464
  pilT Legionella pneumophila strain ERS1305867

46.939

98.847

0.464
  pilU Pseudomonas stutzeri DSM 10701

44.478

96.542

0.429
  pilU Vibrio cholerae strain A1552

40.356

97.118

0.392
  pilU Acinetobacter baylyi ADP1

38.643

97.695

0.378