Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RDV90_RS03665 Genome accession   NZ_CP133578
Coordinates   764542..765522 (+) Length   326 a.a.
NCBI ID   WP_309176226.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain FACU     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 759542..770522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV90_RS03630 - 759914..760240 (+) 327 WP_003862421.1 YggL family protein -
  RDV90_RS03635 - 760297..761013 (+) 717 WP_045407162.1 DUF2884 domain-containing protein -
  RDV90_RS03640 hemW 761204..762352 (-) 1149 WP_309176225.1 radical SAM family heme chaperone HemW -
  RDV90_RS03645 - 762345..762938 (-) 594 WP_023344872.1 XTP/dITP diphosphatase -
  RDV90_RS03650 yggU 762942..763238 (-) 297 WP_008499758.1 DUF167 family protein YggU -
  RDV90_RS03655 - 763235..763801 (-) 567 WP_008499757.1 YggT family protein -
  RDV90_RS03660 - 763823..764524 (-) 702 WP_008499756.1 YggS family pyridoxal phosphate-dependent enzyme -
  RDV90_RS03665 pilT 764542..765522 (+) 981 WP_309176226.1 type IV pilus twitching motility protein PilT Machinery gene
  RDV90_RS03670 ruvX 765533..765949 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  RDV90_RS03675 - 765949..766509 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  RDV90_RS03680 gshB 766592..767539 (-) 948 WP_008499751.1 glutathione synthase -
  RDV90_RS03685 rsmE 767559..768290 (-) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RDV90_RS03690 endA 768342..769049 (-) 708 WP_021242074.1 deoxyribonuclease I -
  RDV90_RS03695 - 769144..769641 (-) 498 WP_059360154.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35527.49 Da        Isoelectric Point: 6.6962

>NTDB_id=875029 RDV90_RS03665 WP_309176226.1 764542..765522(+) (pilT) [Enterobacter roggenkampii strain FACU]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKVWLNDEQQGIWWASGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRVIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLVPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSQAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=875029 RDV90_RS03665 WP_309176226.1 764542..765522(+) (pilT) [Enterobacter roggenkampii strain FACU]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTTCTCCGGACCTCGGGGCGTTGTTAAAAGTGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAGCGGGCAGGTGGATTTTGCCGCGACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTGACGCTACGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGTTATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCACAGCCCGTCATTTGCCGATGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCACCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGGTTCC
TGACTTACAGGACGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACCGGTCAGCAGGCAGGCATGCAGAATTTTGACCAGAGCCAGGCGGAGAGA
CGGGCGCAGGGGCGGCTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Legionella pneumophila strain ERS1305867

47.826

98.773

0.472
  pilT Legionella pneumophila strain Lp02

47.826

98.773

0.472
  pilT Pseudomonas stutzeri DSM 10701

48.265

97.239

0.469
  pilT Neisseria meningitidis 8013

47.5

98.16

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

47.188

98.16

0.463
  pilT Pseudomonas aeruginosa PAK

47.634

97.239

0.463
  pilT Acinetobacter nosocomialis M2

47.022

97.853

0.46
  pilT Acinetobacter baumannii D1279779

47.022

97.853

0.46
  pilT Acinetobacter baumannii strain A118

47.022

97.853

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.887

97.853

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.577

100

0.402
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362