Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GK955_RS03695 Genome accession   NZ_CP133565
Coordinates   770801..771781 (+) Length   326 a.a.
NCBI ID   WP_001055648.1    Uniprot ID   A0A656IH29
Organism   Salmonella enterica strain CFIAFB20140150     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765801..776781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GK955_RS03665 (GK955_03665) - 766379..767386 (+) 1008 WP_000252203.1 DUF1202 domain-containing protein -
  GK955_RS03670 (GK955_03670) hemW 767477..768613 (-) 1137 WP_001096521.1 radical SAM family heme chaperone HemW -
  GK955_RS03675 (GK955_03675) - 768606..769199 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  GK955_RS03680 (GK955_03680) yggU 769207..769497 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  GK955_RS03685 (GK955_03685) - 769494..770060 (-) 567 WP_001094848.1 YggT family protein -
  GK955_RS03690 (GK955_03690) - 770079..770783 (-) 705 WP_000651540.1 YggS family pyridoxal phosphate-dependent enzyme -
  GK955_RS03695 (GK955_03695) pilT 770801..771781 (+) 981 WP_001055648.1 type IV pilus twitching motility protein PilT Machinery gene
  GK955_RS03700 (GK955_03700) - 771913..772599 (+) 687 WP_000098325.1 IclR family transcriptional regulator -
  GK955_RS03705 (GK955_03705) ruvX 772646..773062 (-) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  GK955_RS03710 (GK955_03710) - 773062..773625 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  GK955_RS03715 (GK955_03715) gshB 773841..774788 (-) 948 WP_000593248.1 glutathione synthase -
  GK955_RS03720 (GK955_03720) rsmE 774808..775539 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GK955_RS03725 (GK955_03725) endA 775616..776323 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36155.31 Da        Isoelectric Point: 7.5355

>NTDB_id=874918 GK955_RS03695 WP_001055648.1 770801..771781(+) (pilT) [Salmonella enterica strain CFIAFB20140150]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLTAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=874918 GK955_RS03695 WP_001055648.1 770801..771781(+) (pilT) [Salmonella enterica strain CFIAFB20140150]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGACGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A656IH29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain ERS1305867

44.34

97.546

0.433
  pilT Legionella pneumophila strain Lp02

44.34

97.546

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.245

97.853

0.374
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362