Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q0W94_RS04405 Genome accession   NZ_AP024678
Coordinates   880180..881277 (-) Length   365 a.a.
NCBI ID   WP_297761656.1    Uniprot ID   A0A915V445
Organism   MAG: Thermosynechococcus sp. isolate KatS3mg067     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 875180..886277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0W94_RS04365 - 875351..875500 (+) 150 WP_297761634.1 hypothetical protein -
  Q0W94_RS04370 (KatS3mg067_0858) - 875707..876078 (-) 372 WP_297761637.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  Q0W94_RS04380 (KatS3mg067_0859) - 876348..877499 (+) 1152 WP_297761640.1 cysteine desulfurase family protein -
  Q0W94_RS04385 (KatS3mg067_0860) - 877807..878145 (+) 339 WP_297761643.1 hypothetical protein -
  Q0W94_RS04390 (KatS3mg067_0861) - 878152..878424 (-) 273 WP_297761647.1 DUF3143 domain-containing protein -
  Q0W94_RS04395 (KatS3mg067_0862) - 878421..878900 (-) 480 WP_297761650.1 J domain-containing protein -
  Q0W94_RS04400 (KatS3mg067_0863) - 878909..880129 (-) 1221 WP_297761653.1 type II secretion system F family protein -
  Q0W94_RS04405 (KatS3mg067_0864) pilT 880180..881277 (-) 1098 WP_297761656.1 type IV pilus twitching motility protein PilT Machinery gene
  Q0W94_RS04410 (KatS3mg067_0865) pilB/pilB1 881347..883371 (-) 2025 WP_297761659.1 GspE/PulE family protein Machinery gene
  Q0W94_RS04415 (KatS3mg067_0866) - 883633..883857 (+) 225 WP_297761662.1 hypothetical protein -
  Q0W94_RS04420 (KatS3mg067_0867) - 883858..884055 (+) 198 WP_297761665.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  Q0W94_RS04425 (KatS3mg067_0868) - 884019..885596 (+) 1578 WP_297761668.1 right-handed parallel beta-helix repeat-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40275.44 Da        Isoelectric Point: 6.7579

>NTDB_id=87487 Q0W94_RS04405 WP_297761656.1 880180..881277(-) (pilT) [MAG: Thermosynechococcus sp. isolate KatS3mg067]
MELMIEDLMEQVVANGGSDLHISAGIPPYVRISGKLTPMDYEPLTPEQCQRLIFSMLNNSQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMSERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAISAAETGHLVMGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGVITYEAAMAKSSRPDELQRLIGAGAPAAAVR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=87487 Q0W94_RS04405 WP_297761656.1 880180..881277(-) (pilT) [MAG: Thermosynechococcus sp. isolate KatS3mg067]
ATGGAGTTGATGATTGAGGATCTCATGGAGCAGGTCGTGGCCAACGGTGGTTCAGACCTGCACATTTCAGCAGGTATCCC
CCCCTACGTGCGCATCAGCGGTAAATTAACCCCTATGGATTACGAGCCCCTCACCCCAGAGCAATGCCAGCGCCTGATCT
TCAGCATGCTCAACAATAGCCAACGCAAGCACCTAGAGCAAAACTGGGAGTTGGACTGCTCCTATGGTGTGCGGGGTTTA
GCGCGGTTCCGCGTCAATGTGTATAAAGACCGGGGTACCTATGCCGCCTGCCTGCGGGCTTTAAGTTCCAAGATCCCCAC
CTTTGAACAATTGGGATTGCCCAATATCGTTCGCGAAATGAGTGAGCGGCCACGGGGCCTGATTCTGGTGACTGGGCCGA
CAGGATCAGGAAAAACCACAACCTTGGCGGCAATGATTGACCTGATCAACAAAACCCGTGCCGAGCATATTCTGACCATT
GAAGACCCCATTGAGTTTGTCTATGAACCGATCAAGAGCCTGATCCATCAGCGGCAGGTGGGCGAAGACACCAAGAGTTT
TGCCAATGCCCTGCGGGCAGCGCTGCGGGAAGACCCGGACATTATCCTGGTGGGTGAGATGCGTGACCTGGAAACGATTC
AGCTTGCCATCTCAGCAGCGGAAACGGGTCACTTGGTGATGGGCACTTTGCATACCAGTTCAGCAGCTCAAACCGTTGAC
CGCATGGTGGATGTGTTTCCCCCTGAGCAGCAACAGCAAATTCGTGTCCAGTTGTCCAACTCGTTGGTGGCGGTCTTCAG
CCAAACACTGGTGCCCAAAAAGAATCCGAAGCCCGGTGAATTCGGGCGGATTATGGCCCAAGAGATCATGGTAGTGACCC
CTGCGATCTCCAACCTGATTCGCGAAGGCAAGACATCGCAGATTTATTCGGCAATTCAAACGGGCGGTAAGCTGGGGATG
CAAACCCTCGAAAAAGTCCTCGCCGATTACTATCGTGCGGGGGTAATTACCTATGAGGCAGCAATGGCCAAATCTTCACG
TCCAGATGAGCTGCAACGTCTTATTGGGGCAGGTGCACCTGCGGCGGCAGTGCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.89

95.068

0.512
  pilT Acinetobacter baylyi ADP1

53.026

95.068

0.504
  pilT Legionella pneumophila strain Lp02

53.333

94.521

0.504
  pilT Legionella pneumophila strain ERS1305867

53.333

94.521

0.504
  pilT Pseudomonas stutzeri DSM 10701

52.738

95.068

0.501
  pilT Acinetobacter baumannii strain A118

51.873

95.068

0.493
  pilT Acinetobacter baumannii D1279779

51.873

95.068

0.493
  pilT Acinetobacter nosocomialis M2

51.873

95.068

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.571

95.89

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

93.151

0.468
  pilT Vibrio cholerae strain A1552

50.294

93.151

0.468
  pilT Neisseria gonorrhoeae MS11

48.997

95.616

0.468
  pilT Neisseria meningitidis 8013

48.997

95.616

0.468
  pilU Pseudomonas stutzeri DSM 10701

39.155

97.26

0.381
  pilU Acinetobacter baylyi ADP1

37.892

96.164

0.364

Multiple sequence alignment