Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   Q0W94_RS04410 Genome accession   NZ_AP024678
Coordinates   881347..883371 (-) Length   674 a.a.
NCBI ID   WP_297761659.1    Uniprot ID   A0A915V4N0
Organism   MAG: Thermosynechococcus sp. isolate KatS3mg067     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 876347..888371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0W94_RS04380 (KatS3mg067_0859) - 876348..877499 (+) 1152 WP_297761640.1 cysteine desulfurase family protein -
  Q0W94_RS04385 (KatS3mg067_0860) - 877807..878145 (+) 339 WP_297761643.1 hypothetical protein -
  Q0W94_RS04390 (KatS3mg067_0861) - 878152..878424 (-) 273 WP_297761647.1 DUF3143 domain-containing protein -
  Q0W94_RS04395 (KatS3mg067_0862) - 878421..878900 (-) 480 WP_297761650.1 J domain-containing protein -
  Q0W94_RS04400 (KatS3mg067_0863) - 878909..880129 (-) 1221 WP_297761653.1 type II secretion system F family protein -
  Q0W94_RS04405 (KatS3mg067_0864) pilT 880180..881277 (-) 1098 WP_297761656.1 type IV pilus twitching motility protein PilT Machinery gene
  Q0W94_RS04410 (KatS3mg067_0865) pilB/pilB1 881347..883371 (-) 2025 WP_297761659.1 GspE/PulE family protein Machinery gene
  Q0W94_RS04415 (KatS3mg067_0866) - 883633..883857 (+) 225 WP_297761662.1 hypothetical protein -
  Q0W94_RS04420 (KatS3mg067_0867) - 883858..884055 (+) 198 WP_297761665.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  Q0W94_RS04425 (KatS3mg067_0868) - 884019..885596 (+) 1578 WP_297761668.1 right-handed parallel beta-helix repeat-containing protein -
  Q0W94_RS04430 (KatS3mg067_0869) - 885593..886564 (-) 972 WP_297761670.1 SPOR domain-containing protein -
  Q0W94_RS04435 (KatS3mg067_0870) - 886680..887621 (+) 942 WP_297761672.1 glycosyltransferase -
  Q0W94_RS04440 (KatS3mg067_0871) - 887799..887936 (+) 138 WP_024125599.1 hypothetical protein -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74353.73 Da        Isoelectric Point: 5.4296

>NTDB_id=87488 Q0W94_RS04410 WP_297761659.1 881347..883371(-) (pilB/pilB1) [MAG: Thermosynechococcus sp. isolate KatS3mg067]
MVNTSPSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVAVLQEMTGTTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRSHNLTL
KRMVITLEDYQRLIDPILNKQVAETTASKNAPAAIGEINIEEDVEAIGGLEEIEGEQEVDLAEALRGAEDAPIIALVNKI
LAKALTEGVSDIHVEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVCLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPSAIAR
LSEMGIEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTPEELARFGLSASKDVNLTLYKANKLTPEQIQAAKASGQPICS
KCGGVGYKGRVGVYEIMRVTERLQRLITEGAPTEQIKEAAVEEGMKTLLAYSLELVKQGLTTLEEVERVTFTDTGLEAEL
KAKRKSSLTCRGCGAELAPEWLDCPYCMTPRFLD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=87488 Q0W94_RS04410 WP_297761659.1 881347..883371(-) (pilB/pilB1) [MAG: Thermosynechococcus sp. isolate KatS3mg067]
ATGGTCAACACATCCCCATCGTCCTCTCGCAAAGCACTTACCGTGCGGGGTAGAGCCGCTAGCCCCACAGAACGTGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAAGTCCGGGAAGCAATGAACACCGCCCGCAAAACCGGCAAGTCCCTAGTCG
CCGTGTTGCAGGAGATGACGGGCACCACCATGCCCCCCGATGTGTTACGCCAGTACCACAAGCAGCAGCTTTTTGAACTC
AAAGTGATCTATGGGGTTGATTGCCTTGATCCCGAACTCAATCGTTTTCCCACCGAGCAAATCGAGGAACTGATCAACAC
AATTGTACCCATTGATACCTGCCGCACCTATCAAGTCATCCCCATTGCCAAGCACCTCGATGCCGACCCCCCCTATCTAT
TGGTGGCGATGGTGGATCCCGACAACCTCCAAGCCATTGACAACCTCACCCGTCTGCTGCGCAGTCACAACCTCACGCTC
AAACGGATGGTGATTACCCTAGAGGACTACCAGCGCCTCATTGATCCGATTCTCAATAAACAGGTGGCAGAAACCACCGC
CAGCAAAAATGCCCCGGCGGCGATCGGTGAAATCAACATCGAAGAAGATGTTGAAGCCATCGGCGGCCTCGAAGAGATTG
AAGGGGAGCAGGAGGTTGACCTAGCCGAAGCCCTCAGAGGAGCAGAGGACGCGCCGATTATTGCCCTTGTGAACAAAATC
CTAGCCAAAGCCCTCACGGAAGGGGTGTCTGACATCCACGTTGAACCCCAGGAAGAATACCTACGGATTCGTTTCCGCAA
AGATGGGGTGCTGCACCAAGCCTTTGACCCCCTGCCGAAAAAAATCGTGCCAGCGGTGGTTTCTCGCTTCAAAATCTTGG
CGGACCTCGACATTGCTGAGCGGCGTGCCCCCCAAGATGGCCGCATTCGTAAAATGTTCCAGGGTCGACGCGTGGACTTC
CGGGTAAATACCCTGCCCAGCCGTTGGGGCGAAAAAGTCTGTTTGCGGATTTTGGATAATTCCGCCACCCAGTTGGGCTT
GGATAAACTGATCACGGACCCAGAGAGTCTTGCCATTGTCCGTGAAATGACGAAGCGCCCCTTTGGCTTAATTCTGGTGA
CCGGTCCCACGGGTTCAGGGAAAACAACAACCCTCTACTCTGCCCTTGCTGAGTGCAATAGCCCCGGTGTCAATATCAGT
ACCGCGGAAGACCCGATTGAATACACCCTGCCTGGCTTAACACAGGTGCAGGTGATTCGGGAAAAAGGGATGGACTTTGC
CTCAATTCTACGTGCCTTTCTTCGCCAAGACCCGGATGTGATTCTGGTGGGGGAAACCCGCGACAAGGAAACCGCAAAAA
CCGCTATTGAGGCTGCCTTAACGGGTCACTTAGTTCTGACGACACTACACACCAACGATGCCCCGAGCGCGATCGCCCGT
CTTTCCGAAATGGGCATTGAACCTTTTATGGTTTCGGCTTCCCTAATTGGCGTGGTGGCACAGCGGTTAATGCGGCGAGT
CTGTAGTGAGTGTCGCATTCCCTACACCCCCACCCCCGAAGAGCTGGCACGCTTTGGACTCTCGGCTTCCAAGGACGTCA
ACCTCACCCTCTACAAAGCCAATAAACTCACCCCCGAGCAGATTCAAGCAGCCAAGGCCAGTGGCCAGCCCATCTGCAGT
AAATGTGGTGGTGTAGGCTACAAAGGGCGTGTTGGCGTCTACGAAATTATGCGAGTCACCGAGCGGTTGCAACGCTTAAT
TACAGAAGGTGCCCCCACCGAGCAAATTAAAGAAGCTGCCGTGGAAGAGGGCATGAAAACCCTGTTGGCCTACAGCTTGG
AGCTTGTTAAACAGGGACTGACGACCCTTGAGGAGGTGGAGCGGGTTACCTTTACAGACACTGGGCTAGAGGCAGAGCTG
AAAGCCAAGCGGAAGAGCTCGCTGACCTGTCGCGGTTGTGGCGCCGAACTAGCACCTGAGTGGCTGGATTGTCCCTATTG
CATGACTCCCCGCTTTCTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.939

97.329

0.632
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.924

93.769

0.365

Multiple sequence alignment