Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG25_RS07310 Genome accession   NZ_CP133269
Coordinates   1493089..1494138 (+) Length   349 a.a.
NCBI ID   WP_308083004.1    Uniprot ID   -
Organism   Neobacillus sp. PS2-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1488089..1499138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG25_RS07285 (RCG25_07285) - 1488225..1488695 (-) 471 WP_308082999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RCG25_RS07290 (RCG25_07290) - 1488708..1489142 (-) 435 WP_308083000.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RCG25_RS07295 (RCG25_07295) - 1489373..1490071 (+) 699 WP_308083001.1 hypothetical protein -
  RCG25_RS07300 (RCG25_07300) - 1490092..1491399 (+) 1308 WP_308083002.1 VanW family protein -
  RCG25_RS07305 (RCG25_07305) - 1491415..1493076 (+) 1662 WP_308083003.1 ATPase, T2SS/T4P/T4SS family -
  RCG25_RS07310 (RCG25_07310) pilT 1493089..1494138 (+) 1050 WP_308083004.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG25_RS07315 (RCG25_07315) - 1494131..1495348 (+) 1218 WP_308083005.1 type II secretion system F family protein -
  RCG25_RS07320 (RCG25_07320) - 1495482..1496000 (+) 519 WP_308083006.1 type II secretion system protein -
  RCG25_RS07325 (RCG25_07325) - 1496025..1496789 (+) 765 WP_308083007.1 prepilin peptidase -
  RCG25_RS07330 (RCG25_07330) pilM 1496806..1497792 (+) 987 WP_308083008.1 pilus assembly protein PilM -
  RCG25_RS07335 (RCG25_07335) - 1497797..1498606 (+) 810 WP_308083009.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38758.66 Da        Isoelectric Point: 7.7003

>NTDB_id=873133 RCG25_RS07310 WP_308083004.1 1493089..1494138(+) (pilT) [Neobacillus sp. PS2-9]
MKEKIDAILRAAIEYKASDVHLTVGVPPVMRINGDLRRYGSDKLLQDDTEAMAHAIIPDNMWDIFKEKGELDFSYGVPGS
SRFRVNAYHQRKSVSLALRVVASKVPSIEDLDLPEIVSKLVEKPQGLILVTGPTGSGKSTTLASMIDYMNRTMRKHIITL
EDPIEYLHRHGNSIIDQREVGFDTNNYASGLKAALRQDPDVILVGEMRDLETMSIAITAAETGHLVLGTLHTSSAPTTIN
RIIDVFPSGQQPQIRIQLASVLVGVISQRLFPTIDKKGRKAATEILVNNAAIANLIRNEKIHQIQSTMQTSRAQGMHTLE
MSIRDLIDRSVIQKEAASQYLHEKMMMNG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=873133 RCG25_RS07310 WP_308083004.1 1493089..1494138(+) (pilT) [Neobacillus sp. PS2-9]
ATGAAAGAAAAAATAGATGCGATCTTAAGGGCAGCAATTGAATATAAAGCTTCCGATGTTCATTTAACTGTCGGAGTGCC
GCCGGTCATGCGTATAAATGGTGATTTAAGAAGGTATGGGTCAGACAAATTGCTACAAGATGACACCGAGGCAATGGCAC
ATGCCATTATTCCAGATAATATGTGGGATATTTTTAAAGAAAAAGGGGAGCTTGATTTCTCTTATGGTGTTCCGGGTAGC
TCACGATTCCGTGTAAATGCGTATCACCAACGAAAAAGTGTTTCTTTAGCCCTAAGGGTTGTTGCTTCAAAGGTTCCCTC
AATTGAAGACTTAGATTTACCTGAAATTGTATCTAAGCTAGTAGAAAAGCCTCAAGGTCTTATTCTTGTAACTGGTCCTA
CAGGGAGCGGAAAATCAACGACACTTGCTTCTATGATTGATTATATGAACCGTACGATGCGAAAGCATATCATTACTCTA
GAGGATCCAATCGAGTATCTGCATCGTCATGGAAACTCGATTATTGACCAACGAGAAGTAGGATTTGATACAAATAACTA
TGCCAGTGGCTTAAAAGCAGCGCTTAGACAAGACCCTGATGTTATTCTTGTTGGAGAGATGCGTGACCTGGAGACGATGT
CAATTGCAATCACAGCAGCAGAAACCGGGCATCTTGTACTGGGAACGCTCCATACTTCAAGTGCACCGACGACTATAAAC
CGGATTATCGATGTGTTTCCTTCAGGACAGCAACCACAAATACGTATACAGTTAGCGTCTGTGTTAGTCGGTGTCATTTC
TCAACGATTATTCCCGACTATTGACAAAAAAGGAAGAAAAGCTGCCACTGAGATATTGGTTAATAATGCTGCAATTGCTA
ACCTAATCAGAAATGAGAAAATCCATCAAATTCAAAGTACGATGCAAACTTCCCGTGCCCAGGGAATGCATACGCTCGAG
ATGAGTATTAGAGATCTAATTGATAGAAGTGTGATTCAAAAGGAAGCCGCTTCACAATACTTGCATGAAAAGATGATGAT
GAATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.22

93.983

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.036

94.842

0.456
  pilT Vibrio cholerae strain A1552

48.036

94.842

0.456
  pilT Neisseria gonorrhoeae MS11

47.305

95.702

0.453
  pilT Pseudomonas aeruginosa PAK

46.866

95.989

0.45
  pilT Neisseria meningitidis 8013

47.006

95.702

0.45
  pilT Acinetobacter nosocomialis M2

47.147

95.415

0.45
  pilT Acinetobacter baumannii strain A118

47.147

95.415

0.45
  pilT Acinetobacter baumannii D1279779

47.147

95.415

0.45
  pilT Pseudomonas stutzeri DSM 10701

46.269

95.989

0.444
  pilT Legionella pneumophila strain ERS1305867

45.672

95.989

0.438
  pilT Legionella pneumophila strain Lp02

45.672

95.989

0.438
  pilT Acinetobacter baylyi ADP1

45.646

95.415

0.436
  pilU Pseudomonas stutzeri DSM 10701

40.719

95.702

0.39
  pilU Vibrio cholerae strain A1552

41.667

92.837

0.387
  pilU Acinetobacter baylyi ADP1

41.411

93.41

0.387