Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG19_RS03255 Genome accession   NZ_CP133265
Coordinates   609440..610480 (-) Length   346 a.a.
NCBI ID   WP_308109651.1    Uniprot ID   -
Organism   Neobacillus sp. OS1-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 604440..615480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG19_RS03225 (RCG19_03225) - 604456..605250 (-) 795 WP_308109645.1 pilus assembly protein PilO -
  RCG19_RS03230 (RCG19_03230) - 605247..605906 (-) 660 WP_308109646.1 fimbrial assembly protein -
  RCG19_RS03235 (RCG19_03235) pilM 605909..606895 (-) 987 WP_308109647.1 pilus assembly protein PilM -
  RCG19_RS03240 (RCG19_03240) - 606908..607648 (-) 741 WP_374049605.1 A24 family peptidase -
  RCG19_RS03245 (RCG19_03245) - 607667..608125 (-) 459 WP_308109649.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RCG19_RS03250 (RCG19_03250) - 608233..609438 (-) 1206 WP_308109650.1 type II secretion system F family protein -
  RCG19_RS03255 (RCG19_03255) pilT 609440..610480 (-) 1041 WP_308109651.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG19_RS03260 (RCG19_03260) - 610492..612153 (-) 1662 WP_166238824.1 GspE/PulE family protein -
  RCG19_RS03265 (RCG19_03265) - 612175..613179 (-) 1005 WP_308109652.1 G5 domain-containing protein -
  RCG19_RS03270 (RCG19_03270) - 613199..614005 (-) 807 WP_308109653.1 PRC-barrel domain-containing protein -
  RCG19_RS03275 (RCG19_03275) - 614085..615266 (-) 1182 WP_308109654.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38366.33 Da        Isoelectric Point: 8.9122

>NTDB_id=872956 RCG19_RS03255 WP_308109651.1 609440..610480(-) (pilT) [Neobacillus sp. OS1-2]
MKNKIDLLLRTGFEVKASDIHVTVGTPPIMRINGELRRYGKEPLTPLDTEGIAKAVIPENMWDLFKEKRELDFSYGLQGV
SRFRINAYFQRSCIALAIRVVPTRIPTLDELGLPAIIKKVADKPQGLVLVTGPTGSGKSTTLASIIQYMNQTQRKHVITL
EDPIEYLHKHGGCIIDQREIGFDTNDFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSASATIN
RIVDVFPPTQQSQIRIQLATVLVSIISQRLFPTTDKKGRKAATEILLNNSAIANLIRNEKIHQIVNVMQTSRAEGMHTLE
TNMKELVESGAIAKESTTPYIQEKVQ

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=872956 RCG19_RS03255 WP_308109651.1 609440..610480(-) (pilT) [Neobacillus sp. OS1-2]
ATGAAAAATAAAATTGACCTTCTACTTAGAACCGGATTTGAAGTAAAAGCTTCAGATATCCATGTAACAGTTGGTACCCC
GCCGATCATGAGAATCAATGGAGAGTTAAGGAGATATGGGAAGGAACCATTAACACCGTTAGATACGGAAGGAATAGCCA
AGGCAGTCATCCCTGAAAATATGTGGGATTTGTTTAAAGAAAAAAGGGAATTGGATTTTTCTTACGGATTGCAAGGAGTC
TCAAGGTTTAGGATTAACGCTTATTTTCAACGATCATGTATTGCATTAGCAATAAGGGTTGTGCCTACGAGAATACCGAC
ACTTGATGAACTGGGACTACCTGCTATCATTAAAAAAGTGGCCGATAAACCACAGGGGTTAGTCCTTGTTACCGGTCCTA
CCGGGAGTGGTAAATCTACTACACTTGCTTCGATTATTCAATATATGAATCAAACACAGCGAAAACATGTGATTACGCTT
GAAGACCCAATTGAATACTTGCATAAGCATGGTGGTTGTATCATTGATCAAAGAGAAATAGGATTTGATACGAATGATTT
TGCAAATGGATTAAGAGCAGCATTAAGACAGGATCCTGATGTCATTTTAGTGGGAGAGATGAGGGATTTGGAAACTATCC
AGACCGCTATTACTGCTGCGGAAACCGGCCATTTGGTTCTAGGGACTTTGCATACCTCGAGTGCTTCTGCAACGATTAAT
AGAATAGTAGATGTGTTTCCACCTACACAGCAGTCCCAAATTAGAATTCAGTTGGCGACTGTTTTAGTCTCAATCATTTC
TCAACGGTTATTTCCAACTACTGATAAAAAGGGTAGGAAAGCAGCTACGGAAATCCTACTAAATAACTCAGCCATTGCCA
ATCTCATCAGAAATGAAAAGATTCATCAAATCGTAAATGTGATGCAAACCTCCCGAGCTGAGGGGATGCACACACTTGAA
ACAAATATGAAAGAATTAGTGGAATCAGGTGCCATTGCAAAAGAATCCACTACACCATATATACAGGAAAAGGTGCAATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.829

94.798

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.649

96.243

0.468
  pilT Vibrio cholerae strain A1552

48.649

96.243

0.468
  pilT Neisseria meningitidis 8013

47.59

95.954

0.457
  pilT Neisseria gonorrhoeae MS11

47.147

96.243

0.454
  pilT Legionella pneumophila strain Lp02

46.809

95.087

0.445
  pilT Legionella pneumophila strain ERS1305867

46.809

95.087

0.445
  pilT Pseudomonas aeruginosa PAK

47.5

92.486

0.439
  pilT Acinetobacter baumannii strain A118

46.177

94.509

0.436
  pilT Acinetobacter nosocomialis M2

46.177

94.509

0.436
  pilT Acinetobacter baumannii D1279779

46.177

94.509

0.436
  pilT Acinetobacter baylyi ADP1

45.538

93.931

0.428
  pilT Pseudomonas stutzeri DSM 10701

45.938

92.486

0.425
  pilU Pseudomonas stutzeri DSM 10701

41.463

94.798

0.393
  pilU Vibrio cholerae strain A1552

41.562

92.486

0.384
  pilU Acinetobacter baylyi ADP1

40.994

93.064

0.382