Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG18_RS28740 Genome accession   NZ_CP133264
Coordinates   5843118..5844173 (-) Length   351 a.a.
NCBI ID   WP_308039382.1    Uniprot ID   -
Organism   Clostridium sp. OS1-26     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5838118..5849173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG18_RS28720 (RCG18_28720) ftsZ 5838453..5839583 (-) 1131 WP_308039378.1 cell division protein FtsZ -
  RCG18_RS28725 (RCG18_28725) ftsA 5839600..5840859 (-) 1260 WP_308039379.1 cell division protein FtsA -
  RCG18_RS28730 (RCG18_28730) - 5841027..5842301 (-) 1275 WP_308039380.1 glycosyltransferase family 2 protein -
  RCG18_RS28735 (RCG18_28735) - 5842932..5843066 (+) 135 WP_308039381.1 hypothetical protein -
  RCG18_RS28740 (RCG18_28740) pilT 5843118..5844173 (-) 1056 WP_308039382.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG18_RS28745 (RCG18_28745) sigK 5844538..5845239 (-) 702 WP_178907924.1 RNA polymerase sporulation sigma factor SigK -
  RCG18_RS28750 (RCG18_28750) - 5845314..5846984 (-) 1671 WP_308039383.1 penicillin-binding transpeptidase domain-containing protein -
  RCG18_RS28755 (RCG18_28755) udk 5847002..5847670 (-) 669 WP_308039384.1 uridine kinase -
  RCG18_RS28760 (RCG18_28760) - 5847696..5848922 (-) 1227 WP_308039385.1 U32 family peptidase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38372.29 Da        Isoelectric Point: 7.5962

>NTDB_id=872947 RCG18_RS28740 WP_308039382.1 5843118..5844173(-) (pilT) [Clostridium sp. OS1-26]
MITLKELLAITIKQNASDLHLTVGVPPVIRVNGQLKHVGNEKLQPADTEAYAKEISGEYYDNYIKIGELDTSFSVSGIGR
FRVNLFKQRGSSAIAIRVVGLKIPTLNELQFPSVIKELISNQRGLVLVTGPTGSGKSTTLAAMINEINSSRPAHIITLED
PIEYLHKHNKSIINQREIGKDSLSYANALKSVLREDPDVILVGEMRDLETISIALTAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPYQQQQIKIQLAAVIQGIISQQLLPKADGDGRVAAVEIMVANAAIQNMIREGKTHQLESSIQTGGKYGMKTMDMS
LSELYKKGLVSYESALSYASDRDMLSRIISL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=872947 RCG18_RS28740 WP_308039382.1 5843118..5844173(-) (pilT) [Clostridium sp. OS1-26]
GTGATAACATTAAAAGAATTATTAGCAATAACAATAAAACAGAATGCTTCAGATTTACATTTAACTGTTGGGGTACCTCC
GGTTATAAGAGTAAATGGACAATTAAAGCATGTAGGAAATGAAAAACTACAACCAGCAGATACAGAGGCTTATGCTAAAG
AAATTTCAGGGGAATACTATGATAATTACATAAAAATAGGAGAATTAGACACATCTTTTTCCGTGTCAGGAATTGGAAGA
TTTAGAGTTAACTTATTCAAGCAAAGAGGAAGCTCGGCAATTGCAATAAGAGTCGTTGGATTAAAGATTCCTACGTTAAA
TGAACTTCAATTTCCATCTGTTATAAAGGAACTTATATCCAACCAAAGAGGACTTGTATTAGTAACTGGCCCAACAGGTT
CCGGTAAAAGTACAACTTTGGCTGCAATGATAAATGAAATTAATTCTAGCAGACCAGCTCATATAATTACGCTTGAGGAT
CCAATTGAGTATTTGCATAAGCATAATAAATCTATAATAAATCAAAGAGAAATAGGAAAGGATAGCTTAAGCTATGCTAA
TGCCCTTAAATCTGTTTTAAGAGAAGATCCTGACGTTATACTTGTAGGAGAGATGAGAGATCTAGAAACTATATCCATTG
CTTTAACTGCTGCGGAAACAGGACATTTAGTCTTATCAACACTACATACAATAGGGGCAGCTAAAACGATTGATAGAATA
ATAGACGTGTTTCCTCCGTATCAGCAACAGCAGATTAAGATACAGCTAGCAGCAGTTATTCAAGGCATAATATCTCAGCA
GCTTTTGCCTAAAGCTGATGGAGATGGAAGAGTGGCAGCGGTAGAAATAATGGTTGCTAATGCTGCAATACAGAATATGA
TTAGAGAAGGAAAAACTCATCAACTTGAGTCATCAATACAAACTGGAGGAAAATACGGAATGAAGACTATGGACATGTCT
TTATCCGAACTCTATAAAAAGGGATTAGTATCATATGAATCCGCACTAAGTTATGCATCAGATAGAGATATGCTTTCCAG
AATTATTTCACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

99.715

0.499
  pilT Pseudomonas aeruginosa PAK

51.176

96.866

0.496
  pilT Neisseria gonorrhoeae MS11

49.856

98.86

0.493
  pilT Pseudomonas stutzeri DSM 10701

50.893

95.726

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.296

96.296

0.484
  pilT Vibrio cholerae strain A1552

50.296

96.296

0.484
  pilT Acinetobacter baumannii D1279779

50

96.866

0.484
  pilT Acinetobacter baumannii strain A118

50

96.866

0.484
  pilT Legionella pneumophila strain Lp02

49.271

97.721

0.481
  pilT Legionella pneumophila strain ERS1305867

49.271

97.721

0.481
  pilT Acinetobacter baylyi ADP1

50.148

96.011

0.481
  pilT Acinetobacter nosocomialis M2

49.263

96.581

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.701

99.145

0.473
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.405
  pilU Vibrio cholerae strain A1552

40.237

96.296

0.387
  pilU Acinetobacter baylyi ADP1

39.65

97.721

0.387