Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG22_RS21770 Genome accession   NZ_CP133262
Coordinates   4483696..4484745 (+) Length   349 a.a.
NCBI ID   WP_308104682.1    Uniprot ID   -
Organism   Neobacillus sp. OS1-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4478696..4489745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG22_RS21750 (RCG22_21750) - 4478781..4479728 (+) 948 WP_308104678.1 hypothetical protein -
  RCG22_RS21755 (RCG22_21755) - 4479798..4480604 (+) 807 WP_308104679.1 hypothetical protein -
  RCG22_RS21760 (RCG22_21760) - 4480678..4482006 (+) 1329 WP_308104680.1 G5 domain-containing protein -
  RCG22_RS21765 (RCG22_21765) - 4482022..4483683 (+) 1662 WP_308104681.1 ATPase, T2SS/T4P/T4SS family -
  RCG22_RS21770 (RCG22_21770) pilT 4483696..4484745 (+) 1050 WP_308104682.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG22_RS21775 (RCG22_21775) - 4484738..4485955 (+) 1218 WP_308104683.1 type II secretion system F family protein -
  RCG22_RS21780 (RCG22_21780) - 4486091..4486615 (+) 525 WP_308104684.1 type II secretion system protein -
  RCG22_RS21785 (RCG22_21785) - 4486621..4487397 (+) 777 WP_308104685.1 prepilin peptidase -
  RCG22_RS21790 (RCG22_21790) pilM 4487418..4488404 (+) 987 WP_308104686.1 pilus assembly protein PilM -
  RCG22_RS21795 (RCG22_21795) - 4488404..4489267 (+) 864 WP_308104687.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38844.86 Da        Isoelectric Point: 8.4118

>NTDB_id=872868 RCG22_RS21770 WP_308104682.1 4483696..4484745(+) (pilT) [Neobacillus sp. OS1-33]
MKEKIDAILRASIEYKASDIHLTVGVPPVMRINGDLRRYGTEKLFPVDTEGMAKAIIPEHMWDLFIEKGELDFSYGVPGS
SRFRVNAYHQRKSVSIALRVVASKVPSIEELNLPDIVSKLVEKPQGLILVTGPTGSGKSTTLASMIDYMNRTMRKHIITL
EDPIEYLHRHGNSIIDQREVGFDTNNYASGLKAALRQDPDVILVGEMRDLETMSIAITAAETGHLVLGTLHTSSAPTTIN
RIIDVFPSEQQSQIRIQLASVLVGVISQRLFPTIDKKGRKAATEVLVNNAAIANLIRNEKVHQIQSTMQTSRAQGMHTLE
MSIKDLLERNLIQKEAASQYLHEKMIMNG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=872868 RCG22_RS21770 WP_308104682.1 4483696..4484745(+) (pilT) [Neobacillus sp. OS1-33]
ATGAAAGAAAAAATTGACGCAATTTTACGAGCTTCAATTGAATATAAAGCATCTGATATTCATTTAACCGTCGGTGTTCC
TCCTGTCATGAGGATCAATGGTGATTTAAGAAGGTATGGAACTGAAAAATTATTCCCTGTTGATACAGAAGGTATGGCAA
AAGCAATTATTCCAGAGCATATGTGGGATTTATTTATAGAAAAAGGTGAACTTGATTTCTCCTATGGTGTACCAGGCAGT
TCGCGTTTCCGTGTCAACGCCTATCATCAGCGAAAAAGCGTGTCAATTGCTCTCAGGGTGGTTGCCTCAAAGGTTCCATC
GATTGAGGAATTAAATTTGCCAGATATCGTTTCAAAGTTAGTGGAGAAACCCCAAGGCCTAATACTTGTTACAGGACCAA
CAGGAAGCGGGAAATCAACTACCTTAGCTTCGATGATTGATTACATGAACAGAACGATGAGGAAACATATTATTACCTTA
GAAGATCCAATTGAATATCTTCATCGTCATGGAAATTCAATTATTGATCAGCGAGAAGTTGGCTTTGACACAAATAACTA
TGCAAGCGGCTTGAAGGCTGCCCTTCGTCAAGATCCTGATGTCATACTTGTAGGGGAGATGCGGGACTTAGAAACCATGT
CGATTGCTATTACAGCAGCAGAAACGGGGCACCTTGTCTTAGGAACACTCCATACGTCTAGTGCACCAACTACAATAAAC
CGGATTATCGATGTGTTCCCGTCTGAACAACAGTCTCAAATCCGAATTCAATTGGCGTCTGTCTTAGTAGGGGTAATTTC
GCAACGATTATTCCCTACTATTGATAAAAAGGGTAGAAAAGCAGCCACTGAAGTATTGGTCAACAATGCGGCTATTGCCA
ACCTGATTAGAAATGAAAAGGTACACCAAATTCAAAGCACGATGCAAACCTCCCGAGCCCAAGGGATGCATACACTTGAA
ATGAGTATTAAAGACCTTCTTGAACGTAACCTAATTCAAAAAGAAGCGGCATCACAGTACTTACATGAAAAGATGATAAT
GAATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.134

93.983

0.49
  pilT Pseudomonas aeruginosa PAK

47.463

95.989

0.456
  pilT Pseudomonas stutzeri DSM 10701

46.866

95.989

0.45
  pilT Acinetobacter baumannii D1279779

47.147

95.415

0.45
  pilT Acinetobacter nosocomialis M2

47.147

95.415

0.45
  pilT Acinetobacter baumannii strain A118

47.147

95.415

0.45
  pilT Neisseria gonorrhoeae MS11

46.707

95.702

0.447
  pilT Vibrio cholerae strain A1552

47.13

94.842

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.13

94.842

0.447
  pilT Neisseria meningitidis 8013

46.407

95.702

0.444
  pilT Acinetobacter baylyi ADP1

47.112

94.269

0.444
  pilT Legionella pneumophila strain ERS1305867

45.672

95.989

0.438
  pilT Legionella pneumophila strain Lp02

45.672

95.989

0.438
  pilU Pseudomonas stutzeri DSM 10701

41.617

95.702

0.398
  pilU Vibrio cholerae strain A1552

40.719

95.702

0.39
  pilU Acinetobacter baylyi ADP1

40.798

93.41

0.381