Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RB649_RS08320 Genome accession   NZ_CP133179
Coordinates   1541817..1542902 (-) Length   361 a.a.
NCBI ID   WP_011173990.1    Uniprot ID   Q72H74
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1536817..1547902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB649_RS08305 (RB649_08305) - 1538761..1539393 (-) 633 WP_011173987.1 histidine phosphatase family protein -
  RB649_RS08310 (RB649_08310) purM 1539390..1540391 (-) 1002 WP_011173988.1 phosphoribosylformylglycinamidine cyclo-ligase -
  RB649_RS08315 (RB649_08315) gatB 1540397..1541806 (-) 1410 WP_011173989.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  RB649_RS08320 (RB649_08320) pilT 1541817..1542902 (-) 1086 WP_011173990.1 type IV pilus twitching motility protein PilT Machinery gene
  RB649_RS08325 (RB649_08325) pilF 1542915..1545584 (-) 2670 WP_011173991.1 type IV pilus assembly ATPase PilB Machinery gene
  RB649_RS08330 (RB649_08330) - 1545581..1546066 (-) 486 WP_011173992.1 YqeG family HAD IIIA-type phosphatase -
  RB649_RS08335 (RB649_08335) pgeF 1546101..1546829 (-) 729 WP_011173993.1 peptidoglycan editing factor PgeF -
  RB649_RS08340 (RB649_08340) surE 1546826..1547581 (-) 756 WP_011173994.1 5'/3'-nucleotidase SurE -
  RB649_RS08345 (RB649_08345) - 1547640..1547846 (-) 207 WP_011173995.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39948.26 Da        Isoelectric Point: 7.5114

>NTDB_id=872296 RB649_RS08320 WP_011173990.1 1541817..1542902(-) (pilT) [Thermus thermophilus HB27]
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=872296 RB649_RS08320 WP_011173990.1 1541817..1542902(-) (pilT) [Thermus thermophilus HB27]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGGCGCTCGCGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTCCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAAAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAAAGGGGAAGCGTGGGGGGGGTTCTAAGGGTGGTGCCGAGCGCCGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGAGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCCGGCAAGAGCACCACCCTGGCCTCCATGATTGACTACATCAACGAACGCAAGGCCTGCCACATCGTC
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAGACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCTACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTCTACCGGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72H74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.272

97.784

0.668
  pilT Pseudomonas aeruginosa PAK

54.07

95.291

0.515
  pilT Pseudomonas stutzeri DSM 10701

53.779

95.291

0.512
  pilT Acinetobacter baylyi ADP1

53.779

95.291

0.512
  pilT Acinetobacter baumannii strain A118

52.754

95.568

0.504
  pilT Acinetobacter nosocomialis M2

52.754

95.568

0.504
  pilT Acinetobacter baumannii D1279779

52.754

95.568

0.504
  pilT Neisseria meningitidis 8013

52.663

93.629

0.493
  pilT Neisseria gonorrhoeae MS11

51.929

93.352

0.485
  pilT Legionella pneumophila strain ERS1305867

50.581

95.291

0.482
  pilT Legionella pneumophila strain Lp02

50.581

95.291

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.672

91.136

0.471
  pilT Vibrio cholerae strain A1552

51.672

91.136

0.471
  pilU Pseudomonas stutzeri DSM 10701

46.647

95.014

0.443
  pilU Acinetobacter baylyi ADP1

42.899

95.568

0.41
  pilU Vibrio cholerae strain A1552

45.484

85.873

0.391