Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q9R17_RS10840 Genome accession   NZ_CP133160
Coordinates   2434859..2435986 (-) Length   375 a.a.
NCBI ID   WP_308154656.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 24(2023)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2429859..2440986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9R17_RS10825 (Q9R17_10825) - 2430843..2432264 (-) 1422 WP_308154653.1 DEAD/DEAH box helicase -
  Q9R17_RS10830 (Q9R17_10830) - 2433144..2434133 (+) 990 WP_308154654.1 fumarylacetoacetate hydrolase family protein -
  Q9R17_RS10835 (Q9R17_10835) maiA 2434148..2434816 (+) 669 WP_308154655.1 maleylacetoacetate isomerase -
  Q9R17_RS10840 (Q9R17_10840) pilU 2434859..2435986 (-) 1128 WP_308154656.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9R17_RS10845 (Q9R17_10845) - 2436109..2436474 (+) 366 WP_308158327.1 DUF4398 domain-containing protein -
  Q9R17_RS10850 (Q9R17_10850) - 2436471..2437355 (+) 885 WP_308154657.1 hypothetical protein -
  Q9R17_RS10855 (Q9R17_10855) - 2437680..2437916 (+) 237 WP_308154658.1 YdcH family protein -
  Q9R17_RS10860 (Q9R17_10860) - 2438209..2439579 (+) 1371 WP_308154659.1 pyridoxal-phosphate dependent enzyme -
  Q9R17_RS10865 (Q9R17_10865) - 2439703..2440881 (+) 1179 WP_308154660.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41437.66 Da        Isoelectric Point: 6.3735

>NTDB_id=872154 Q9R17_RS10840 WP_308154656.1 2434859..2435986(-) (pilU) [Stenotrophomonas sp. 24(2023)]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKLSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRAVISQRLVKNKDGRRLPATEVLINTPLIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIDQEEALRAADSRDGLALKFRLSEGSSGEHDPYADYSAGQPTITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=872154 Q9R17_RS10840 WP_308154656.1 2434859..2435986(-) (pilU) [Stenotrophomonas sp. 24(2023)]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACCGGGGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTATCCGCTGGGCAATACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAGCGCGAGCTGGAACTGAACATGGCCATCGCCCTGCCCGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCAAGATTCCCAGCATCGA
AGAGCTGAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCCACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACGACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGCTGTCCATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCCCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACGATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACCGCAACGTGCTGATGAACCTGTCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGACGGGCGCCGGCTGCCGGCCACCGAGGTGCTGATCAACACCCCGCTGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCCGCGATGGAGGCCTCGCTGGAGGAAGGCATGGAAAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGACCAGGAAGAAGCGCTGCGCGCGGCCGATTCACGCGACGGGCTGGCGTTGAA
GTTCCGCCTGTCCGAGGGCAGCAGCGGCGAACACGACCCGTATGCGGATTACTCGGCTGGCCAGCCGACGATCACCCACG
GATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.733

0.499
  pilU Acinetobacter baylyi ADP1

50.565

94.4

0.477
  pilU Vibrio cholerae strain A1552

48.098

98.133

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.81

95.467

0.437
  pilT Pseudomonas aeruginosa PAK

43.491

90.133

0.392
  pilT Acinetobacter baumannii strain A118

42.308

90.133

0.381
  pilT Acinetobacter nosocomialis M2

42.308

90.133

0.381
  pilT Acinetobacter baumannii D1279779

42.308

90.133

0.381
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Pseudomonas stutzeri DSM 10701

42.012

90.133

0.379
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

41.441

88.8

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

88.8

0.368
  pilT Neisseria gonorrhoeae MS11

39.769

92.533

0.368
  pilT Neisseria meningitidis 8013

39.769

92.533

0.368