NTDB

Overview

Name	pilA/pilAI	Type	Machinery gene
Locus tag	Q9R17_RS04060	Genome accession	NZ_CP133160
Coordinates	915136..915555 (-)	Length	139 a.a.
NCBI ID	WP_308157168.1	Uniprot ID	-
Organism	Stenotrophomonas sp. 24(2023)
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 910136..920555

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q9R17_RS04045 (Q9R17_04045)	pilR	911255..912628 (+)	1374	WP_308157166.1	sigma-54 dependent transcriptional regulator	Regulator
Q9R17_RS04050 (Q9R17_04050)	pilB	912678..914402 (-)	1725	WP_308157167.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Q9R17_RS04055 (Q9R17_04055)	-	914620..915027 (-)	408	WP_308158271.1	pilin	-
Q9R17_RS04060 (Q9R17_04060)	pilA/pilAI	915136..915555 (-)	420	WP_308157168.1	pilin	Machinery gene
Q9R17_RS04065 (Q9R17_04065)	pilC	915908..917167 (+)	1260	WP_308157169.1	type II secretion system F family protein	Machinery gene
Q9R17_RS04070 (Q9R17_04070)	-	917175..918038 (+)	864	WP_308157170.1	A24 family peptidase	-
Q9R17_RS04075 (Q9R17_04075)	coaE	918049..918660 (+)	612	WP_308157171.1	dephospho-CoA kinase	-
Q9R17_RS04080 (Q9R17_04080)	-	918718..919395 (-)	678	WP_308157172.1	response regulator transcription factor	-
Q9R17_RS04085 (Q9R17_04085)	-	919404..919880 (-)	477	WP_308157173.1	hypothetical protein	-

Sequence

Protein

Download Length: 139 a.a. Molecular weight: 14616.87 Da Isoelectric Point: 8.1004

                                >NTDB_id=872147 Q9R17_RS04060 WP_308157168.1 915136..915555(-) (pilA/pilAI) [Stenotrophomonas sp. 24(2023)]

MKNQKGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQVTAALAEITPGKTQFEIFVNEGKAADDFTATKLGLPTAPTTR

CTSFTITAGAEGSIACTTIKGSPKAAGNITWKRAADGTWTCNTSVTDDKYYPVGCTKAG

Nucleotide

Download Length: 420 bp

                                >NTDB_id=872147 Q9R17_RS04060 WP_308157168.1 915136..915555(-) (pilA/pilAI) [Stenotrophomonas sp. 24(2023)]

ATGAAGAACCAGAAGGGCTTTACCCTGATCGAACTGATGATCGTCGTTGCGATCATCGCCATCCTGGCCGCCATCGCGCT

GCCGATGTACCAGGACTACGTGGCCAAGTCGCAGGTCACCGCTGCGCTGGCTGAAATCACCCCCGGCAAGACCCAGTTCG

AGATCTTCGTGAACGAAGGCAAGGCGGCGGACGATTTCACCGCGACCAAGCTGGGTCTGCCGACCGCGCCGACCACCCGT

TGCACCAGCTTCACCATCACCGCTGGCGCTGAAGGTTCGATCGCCTGCACCACCATCAAGGGCAGCCCGAAGGCGGCTGG

CAACATCACCTGGAAGCGTGCGGCCGACGGTACCTGGACCTGCAACACCTCGGTGACCGACGACAAGTACTATCCGGTTG

GCTGCACCAAGGCGGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	53.285	98.561	0.525
pilA	Pseudomonas aeruginosa PAK	45.695	100	0.496
pilA	Acinetobacter baumannii strain A118	48.227	100	0.489
pilA/pilAII	Pseudomonas stutzeri DSM 10701	46.617	95.683	0.446
pilA	Vibrio cholerae strain A1552	42.466	100	0.446
pilA	Vibrio cholerae C6706	42.466	100	0.446
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.466	100	0.446
comP	Acinetobacter baylyi ADP1	37.908	100	0.417
pilA	Ralstonia pseudosolanacearum GMI1000	34.969	100	0.41
pilA2	Legionella pneumophila str. Paris	39.552	96.403	0.381
pilA2	Legionella pneumophila strain ERS1305867	39.552	96.403	0.381
pilA	Haemophilus influenzae 86-028NP	37.778	97.122	0.367
pilE	Neisseria gonorrhoeae MS11	37.778	97.122	0.367

Detailed information NTG0872147