Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q9R17_RS04050 Genome accession   NZ_CP133160
Coordinates   912678..914402 (-) Length   574 a.a.
NCBI ID   WP_308157167.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 24(2023)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 907678..919402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9R17_RS04035 (Q9R17_04035) sucC 908070..909239 (-) 1170 WP_125362066.1 ADP-forming succinate--CoA ligase subunit beta -
  Q9R17_RS04040 (Q9R17_04040) - 909580..911193 (+) 1614 WP_308157165.1 ATP-binding protein -
  Q9R17_RS04045 (Q9R17_04045) pilR 911255..912628 (+) 1374 WP_308157166.1 sigma-54 dependent transcriptional regulator Regulator
  Q9R17_RS04050 (Q9R17_04050) pilB 912678..914402 (-) 1725 WP_308157167.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q9R17_RS04055 (Q9R17_04055) - 914620..915027 (-) 408 WP_308158271.1 pilin -
  Q9R17_RS04060 (Q9R17_04060) pilA/pilAI 915136..915555 (-) 420 WP_308157168.1 pilin Machinery gene
  Q9R17_RS04065 (Q9R17_04065) pilC 915908..917167 (+) 1260 WP_308157169.1 type II secretion system F family protein Machinery gene
  Q9R17_RS04070 (Q9R17_04070) - 917175..918038 (+) 864 WP_308157170.1 A24 family peptidase -
  Q9R17_RS04075 (Q9R17_04075) coaE 918049..918660 (+) 612 WP_308157171.1 dephospho-CoA kinase -
  Q9R17_RS04080 (Q9R17_04080) - 918718..919395 (-) 678 WP_308157172.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62039.99 Da        Isoelectric Point: 5.8686

>NTDB_id=872146 Q9R17_RS04050 WP_308157167.1 912678..914402(-) (pilB) [Stenotrophomonas sp. 24(2023)]
MNAVTTANLVGITGIARRLVQDGALDEATARDAMAKATAARQPLPQWFAQNKLVSASQLAAAGSVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTMGDALGGD
DDGMANLDVSGGDEEGSSDTGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKLN
QRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQTLFLE
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCTQCKQRAELPGHAL
LAEGFTQAQIDAGIQLYEAVGCDECTGGYKGRTGIYQVMPMTDEIATIILAGGNALQIAEAAQAIGVNDLRQSALVKVAA
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=872146 Q9R17_RS04050 WP_308157167.1 912678..914402(-) (pilB) [Stenotrophomonas sp. 24(2023)]
ATGAACGCCGTCACCACCGCCAACCTCGTCGGTATCACCGGCATCGCCCGCCGCCTTGTGCAGGACGGTGCGCTGGATGA
AGCCACCGCCCGTGATGCGATGGCCAAGGCCACTGCCGCGCGGCAGCCGCTGCCGCAGTGGTTTGCCCAGAACAAGCTGG
TCAGTGCCTCGCAGCTGGCCGCGGCCGGCTCGGTCGAATTCGGCATGCCGCTGTTCGATGTGTCCACCTTCGATGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGTGGCGGCAAGCT
GTTCGTGGGGACCAGCAACCCCACCCACGCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGACGAAGACCAGATCCGCCGCACGCTGGAACAGTGGCATGCCAGCCACGACACCATGGGCGATGCGCTGGGGGGCGAT
GACGATGGCATGGCCAATCTCGACGTCAGCGGCGGCGACGAGGAGGGCAGTTCCGACACGGGCATCGACGCCAAGGGGGA
TGACACGCCCGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCCATCCGCAAGGGGGCATCGGACATCCACTTCGAGC
CCTATGAAGACGACTACCGGGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCACGCGCGCCGGTGAAGCTGAAC
CAGCGCATCGCCGCGCGACTGAAGGTGATGGCGCAGCTGGACATCGCCGAAAAGCGCGTGCCGCAGGACGGGCGCATCAA
GCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAAAAGGTAGTGCTGC
GTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAACCGGACCAGCAGACGCTGTTCCTGGAA
GCGATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACCGCGCT
GGGCATCCTCAACGATGAGACCCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGCCTGCCCGGCGTCAACCAGG
TGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACATCATCATG
GTGGGTGAAATCCGCGACCTGGAAACGGCCGAGATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCCACGCT
GCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCTCCGTACAACATCACCAGTTCGGTCA
CGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGCACGCAGTGCAAGCAGCGTGCGGAACTGCCCGGGCATGCGCTG
CTGGCCGAAGGCTTCACCCAGGCACAGATCGATGCGGGCATCCAGCTGTACGAGGCGGTGGGATGCGATGAATGCACCGG
CGGCTACAAGGGGCGCACGGGCATCTACCAGGTGATGCCGATGACCGATGAGATCGCCACCATCATCCTGGCCGGTGGCA
ATGCCCTGCAGATTGCCGAGGCGGCGCAGGCCATCGGCGTGAACGACCTGCGCCAGTCGGCGCTGGTGAAGGTCGCTGCC
GGCGTCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.633

100

0.559
  pilB Acinetobacter baumannii D1279779

56.537

98.606

0.557
  pilB Legionella pneumophila strain ERS1305867

52.734

98.78

0.521
  pilB Vibrio cholerae strain A1552

49.825

99.652

0.497
  pilF Neisseria gonorrhoeae MS11

49.293

98.606

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.986

98.258

0.462
  pilB Vibrio campbellii strain DS40M4

45.184

99.477

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

36.782

100

0.39
  pilF Thermus thermophilus HB27

37.634

97.213

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.024

92.857

0.362