Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QU599_RS02100 Genome accession   NZ_CP133017
Coordinates   443529..444569 (+) Length   346 a.a.
NCBI ID   WP_308637369.1    Uniprot ID   -
Organism   Paenibacillus silvisoli strain TW38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438529..449569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QU599_RS02075 - 438837..439946 (+) 1110 WP_308637364.1 hypothetical protein -
  QU599_RS02080 - 439960..440277 (+) 318 WP_308637365.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QU599_RS02085 - 440314..440868 (+) 555 WP_308637366.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QU599_RS02090 - 440902..441834 (+) 933 WP_308637367.1 VanW family protein -
  QU599_RS02095 - 441856..443517 (+) 1662 WP_308637368.1 ATPase, T2SS/T4P/T4SS family -
  QU599_RS02100 pilT 443529..444569 (+) 1041 WP_308637369.1 type IV pilus twitching motility protein PilT Machinery gene
  QU599_RS02105 - 444570..445787 (+) 1218 WP_308637370.1 type II secretion system F family protein -
  QU599_RS02110 - 445836..446384 (+) 549 WP_308637371.1 type II secretion system protein -
  QU599_RS02115 - 446416..446946 (+) 531 WP_308637372.1 type II secretion system protein -
  QU599_RS02120 - 447018..447779 (+) 762 WP_308637373.1 prepilin peptidase -
  QU599_RS02125 pilM 447802..448794 (+) 993 WP_308637374.1 pilus assembly protein PilM -
  QU599_RS02130 - 448791..449387 (+) 597 WP_308637375.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38494.22 Da        Isoelectric Point: 6.5665

>NTDB_id=871331 QU599_RS02100 WP_308637369.1 443529..444569(+) (pilT) [Paenibacillus silvisoli strain TW38]
MKATIDELLLTAFHKKASDIHLTAGTPPIFRINGDLRDHGDQPLSDRETEEMARAMIPEAQWESFLERGEIDFAYSIPQV
SRFRVNAFSQRGEMSVAIRIIPTAIPSLEQLQLPDVLHKVCSKTQGLFLVTGPTGSGKSTTLASMLDYMNKTMRKHIVTL
EDPIEYLHKHDRSIIVQREVGADTRSFANGLRAALRQDPDVILVGELRDLDTISTAISAAETGHLVLGTLHTTDAPSTID
RIIDVFPPGQQQQIRIQLASVLIGVLSQRLLPTMDYAGRRAATEVLVNTAGVANLIRSEKVHQIPNVMQTGRTLGMHTLE
MSLHELMQHYIISREVAQPYLREVTV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=871331 QU599_RS02100 WP_308637369.1 443529..444569(+) (pilT) [Paenibacillus silvisoli strain TW38]
ATGAAAGCAACCATTGATGAGCTGCTGCTGACGGCTTTCCATAAGAAAGCGTCCGATATTCACTTGACCGCGGGCACTCC
GCCAATCTTCCGAATCAATGGGGATTTGCGGGACCACGGAGATCAGCCGCTTTCCGATCGGGAAACCGAAGAGATGGCGC
GGGCGATGATCCCGGAGGCGCAATGGGAATCGTTTCTGGAAAGAGGCGAGATCGATTTTGCTTATTCGATCCCGCAAGTC
AGCCGATTTCGCGTAAATGCCTTCTCCCAACGCGGGGAGATGAGCGTGGCCATCCGGATCATTCCGACCGCCATCCCCTC
CTTGGAGCAGCTTCAGCTGCCGGACGTCTTGCATAAGGTGTGCTCCAAAACGCAGGGCTTGTTTCTCGTAACCGGCCCTA
CGGGGAGCGGCAAATCTACGACGCTCGCATCCATGCTCGATTACATGAATAAGACGATGCGCAAGCATATTGTCACGCTC
GAGGATCCGATCGAGTATTTGCACAAGCATGACCGGAGCATCATCGTGCAGCGCGAGGTCGGCGCCGATACGCGAAGCTT
CGCGAACGGACTGCGGGCGGCGCTCCGTCAGGACCCGGATGTCATTCTCGTCGGCGAACTGCGCGACCTCGATACGATAT
CGACCGCCATTTCGGCGGCGGAGACGGGCCATCTGGTGCTGGGCACCCTCCATACGACAGATGCGCCGTCCACGATCGAC
CGGATCATTGACGTCTTTCCGCCGGGCCAGCAGCAGCAGATCCGCATTCAGCTGGCGTCCGTCCTGATCGGCGTGCTGTC
GCAGCGGCTGCTGCCGACCATGGATTACGCCGGCAGACGCGCGGCGACGGAGGTGCTCGTGAACACCGCCGGCGTGGCCA
ATCTGATTCGCAGCGAGAAGGTGCATCAAATTCCGAACGTGATGCAGACAGGCCGGACGTTGGGGATGCACACGCTGGAG
ATGTCGCTTCATGAGCTGATGCAGCACTATATCATCTCCAGGGAAGTCGCTCAACCGTATTTGCGGGAGGTGACGGTATA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.368

95.087

0.488
  pilT Neisseria meningitidis 8013

48.358

96.821

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.904

96.532

0.462
  pilT Vibrio cholerae strain A1552

47.904

96.532

0.462
  pilT Neisseria gonorrhoeae MS11

47.761

96.821

0.462
  pilT Pseudomonas aeruginosa PAK

47.605

96.532

0.46
  pilT Legionella pneumophila strain Lp02

46.707

96.532

0.451
  pilT Legionella pneumophila strain ERS1305867

46.707

96.532

0.451
  pilT Pseudomonas stutzeri DSM 10701

45.723

97.977

0.448
  pilT Acinetobacter baylyi ADP1

46.407

96.532

0.448
  pilT Acinetobacter baumannii strain A118

45.808

96.532

0.442
  pilT Acinetobacter nosocomialis M2

45.808

96.532

0.442
  pilT Acinetobacter baumannii D1279779

45.808

96.532

0.442
  pilU Pseudomonas stutzeri DSM 10701

43.544

96.243

0.419
  pilU Acinetobacter baylyi ADP1

42.249

95.087

0.402
  pilU Vibrio cholerae strain A1552

41.304

93.064

0.384