Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAH45_RS01625 Genome accession   NZ_CP132595
Coordinates   369823..370878 (-) Length   351 a.a.
NCBI ID   WP_068406410.1    Uniprot ID   A0A2I0ELL4
Organism   Psychrobacter sp. van23A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364823..375878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAH45_RS01600 (RAH45_01600) - 365682..366821 (+) 1140 WP_068406401.1 hypothetical protein -
  RAH45_RS01605 (RAH45_01605) - 366809..367207 (+) 399 WP_227514961.1 RnfH family protein -
  RAH45_RS01610 (RAH45_01610) - 367230..367649 (-) 420 WP_068406454.1 outer membrane protein assembly factor BamE -
  RAH45_RS01615 (RAH45_01615) fur 367962..368396 (+) 435 WP_068406404.1 ferric iron uptake transcriptional regulator -
  RAH45_RS01620 (RAH45_01620) pilU 368532..369653 (-) 1122 WP_068406407.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAH45_RS01625 (RAH45_01625) pilT 369823..370878 (-) 1056 WP_068406410.1 type IV pilus twitching motility protein PilT Machinery gene
  RAH45_RS01630 (RAH45_01630) - 371140..371904 (+) 765 WP_082894340.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAH45_RS01635 (RAH45_01635) - 372126..373403 (-) 1278 WP_198328760.1 hemolysin family protein -
  RAH45_RS01640 (RAH45_01640) - 373699..375402 (-) 1704 WP_102077573.1 NAD+ synthase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39266.19 Da        Isoelectric Point: 6.8981

>NTDB_id=869533 RAH45_RS01625 WP_068406410.1 369823..370878(-) (pilT) [Psychrobacter sp. van23A]
MAEKHRLNIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPEMTHQVVHQLIYDIINDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGEGMI
TLDQTLKNLVSKGTISKDVARPYAKQPEAFI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=869533 RAH45_RS01625 WP_068406410.1 369823..370878(-) (pilT) [Psychrobacter sp. van23A]
ATGGCTGAAAAGCATCGTTTAAACATCGAAGACTTGTTGCGCTTTACCGTTAAGCACAAAGCGTCGGATTTACATATTTC
AGCCGGTATGCCAGCGATGATTCGTGTCGATGGTGAGATGACCCGTATTAATATGCCTGAAATGACGCATCAAGTGGTGC
ATCAGCTTATTTATGACATCATAAATGATAAGCAACGCGCTGACTTTGAAGAGTTCTTTGAGACCGACTTCTCTTTTGAG
ATTCCAAATCTAGCACGTTTTCGTGTCAACGCCTTTAATCAAAACCGCGGCGCTGGCGCTGTTTTTCGAACCATTCCTTC
TAAAGTTCTCACTATGGAAGACTTAGGTATGGGTGACGTCTTTAAACGAGTATCCGACTTTAAACGCGGTGTGGTACTGG
TCACGGGGCCAACGGGTTCAGGGAAATCTACCACGCTTGCTGCCATGATCGATTATATCAATGAAACCCGTAAAGAGCAT
ATTTTAACCATCGAAGACCCGATTGAATTTGTTCATGAGTCTAAAAAGAGCTTGATTAACCAACGTGAAGTCCACCGCGA
TACTTTGGGTTTCGAGCCAGCATTGCGCTCGGCTCTACGTGAAGATCCAGACGTTATCCTCGTTGGTGAGTTACGTGACC
TTGAGACCATTCGCTTGGCATTGACCGCCGCTGAAACAGGGCATTTGGTCTTTGGCACCTTGCACACCAGCTCTGCTGCC
AAAACCATTGATCGCGTGATTGACGTCTTCCCTGCAGCCGAAAAAGATATGATACGGGCGATGCTCTCAGAGTCGCTACA
AGCGGTCATCTCACAAACGCTACTACGCCGCCAAACCGGCGGACGTATTGCTGCTCATGAAATCATGCTGGGCACGCCTG
CGATTCGTAACTTGATACGTGAGAATAAAATCGCGCAGATGTATTCTGCTATCCAAACTGGTGCTGGTGAAGGCATGATT
ACTCTTGATCAAACGCTTAAAAACTTGGTTTCAAAAGGCACAATCAGTAAAGATGTCGCTCGCCCTTATGCCAAGCAGCC
TGAAGCCTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I0ELL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.841

98.291

0.775
  pilT Acinetobacter baumannii D1279779

78.551

98.291

0.772
  pilT Acinetobacter baumannii strain A118

78.551

98.291

0.772
  pilT Pseudomonas aeruginosa PAK

77.616

98.006

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.035

98.006

0.755
  pilT Acinetobacter baylyi ADP1

75.872

98.006

0.744
  pilT Legionella pneumophila strain Lp02

71.221

98.006

0.698
  pilT Legionella pneumophila strain ERS1305867

71.221

98.006

0.698
  pilT Neisseria meningitidis 8013

64.348

98.291

0.632
  pilT Neisseria gonorrhoeae MS11

63.768

98.291

0.627
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.869

93.732

0.627
  pilT Vibrio cholerae strain A1552

66.869

93.732

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

98.291

0.499
  pilU Acinetobacter baylyi ADP1

41.691

97.721

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.708

96.581

0.393
  pilU Vibrio cholerae strain A1552

42.415

92.023

0.39