Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAM09_RS20680 Genome accession   NZ_CP132377
Coordinates   4306331..4307353 (-) Length   340 a.a.
NCBI ID   WP_033632094.1    Uniprot ID   -
Organism   Serratia marcescens strain KZ11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4301331..4312353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM09_RS20650 (RAM09_20655) - 4302132..4302644 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  RAM09_RS20655 (RAM09_20660) endA 4302746..4303441 (+) 696 WP_015379043.1 deoxyribonuclease I -
  RAM09_RS20660 (RAM09_20665) rsmE 4303511..4304242 (+) 732 WP_033632097.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RAM09_RS20665 (RAM09_20670) gshB 4304253..4305203 (+) 951 WP_048325369.1 glutathione synthase -
  RAM09_RS20670 (RAM09_20675) - 4305349..4305912 (+) 564 WP_103687493.1 YqgE/AlgH family protein -
  RAM09_RS20675 (RAM09_20680) ruvX 4305912..4306334 (+) 423 WP_033632095.1 Holliday junction resolvase RuvX -
  RAM09_RS20680 (RAM09_20685) pilT 4306331..4307353 (-) 1023 WP_033632094.1 type IV pilus twitching motility protein PilT Machinery gene
  RAM09_RS20685 (RAM09_20690) - 4307374..4308081 (+) 708 WP_103687494.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAM09_RS20690 (RAM09_20695) proC 4308101..4308922 (+) 822 WP_048325374.1 pyrroline-5-carboxylate reductase -
  RAM09_RS20695 (RAM09_20700) - 4308954..4309508 (+) 555 WP_033632091.1 YggT family protein -
  RAM09_RS20700 (RAM09_20705) yggU 4309505..4309798 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  RAM09_RS20705 (RAM09_20710) - 4309939..4310532 (+) 594 WP_004937429.1 XTP/dITP diphosphatase -
  RAM09_RS20710 (RAM09_20715) hemW 4310525..4311667 (+) 1143 WP_048325377.1 radical SAM family heme chaperone HemW -
  RAM09_RS20715 (RAM09_20720) - 4311706..4312140 (-) 435 WP_019456096.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36377.63 Da        Isoelectric Point: 7.2888

>NTDB_id=868817 RAM09_RS20680 WP_033632094.1 4306331..4307353(-) (pilT) [Serratia marcescens strain KZ11]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGVSLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAAEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=868817 RAM09_RS20680 WP_033632094.1 4306331..4307353(-) (pilT) [Serratia marcescens strain KZ11]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCATTGGCGGCGGCGCCAACGCTGACGGCGCAGGGCGCGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAGTTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCAGCCATCGTCCCGGCGTTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGTTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCGGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGAGTGCGCGGCGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria gonorrhoeae MS11

49.702

98.824

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

48.955

98.529

0.482
  pilT Legionella pneumophila strain Lp02

48.955

98.529

0.482
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Pseudomonas stutzeri DSM 10701

47.917

98.824

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.279

99.118

0.379