Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q9K10_RS21750 Genome accession   NZ_CP132197
Coordinates   4494867..4495889 (-) Length   340 a.a.
NCBI ID   WP_060422991.1    Uniprot ID   -
Organism   Serratia marcescens strain GES6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4489867..4500889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9K10_RS21720 (Q9K10_21740) - 4490668..4491180 (+) 513 WP_060422995.1 SprT family zinc-dependent metalloprotease -
  Q9K10_RS21725 (Q9K10_21745) endA 4491282..4491977 (+) 696 WP_060422993.1 deoxyribonuclease I -
  Q9K10_RS21730 (Q9K10_21750) rsmE 4492047..4492778 (+) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q9K10_RS21735 (Q9K10_21755) gshB 4492789..4493739 (+) 951 WP_033635939.1 glutathione synthase -
  Q9K10_RS21740 (Q9K10_21760) - 4493885..4494448 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  Q9K10_RS21745 (Q9K10_21765) ruvX 4494448..4494870 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  Q9K10_RS21750 (Q9K10_21770) pilT 4494867..4495889 (-) 1023 WP_060422991.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9K10_RS21755 (Q9K10_21775) - 4495910..4496617 (+) 708 WP_060422989.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9K10_RS21760 (Q9K10_21780) proC 4496637..4497458 (+) 822 WP_033635943.1 pyrroline-5-carboxylate reductase -
  Q9K10_RS21765 (Q9K10_21785) - 4497490..4498044 (+) 555 WP_033635944.1 YggT family protein -
  Q9K10_RS21770 (Q9K10_21790) yggU 4498041..4498331 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  Q9K10_RS21775 (Q9K10_21795) - 4498349..4498942 (+) 594 WP_033635946.1 XTP/dITP diphosphatase -
  Q9K10_RS21780 (Q9K10_21800) hemW 4498935..4500077 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  Q9K10_RS21785 (Q9K10_21805) - 4500112..4500198 (-) 87 Protein_4259 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36444.72 Da        Isoelectric Point: 6.8881

>NTDB_id=867570 Q9K10_RS21750 WP_060422991.1 4494867..4495889(-) (pilT) [Serratia marcescens strain GES6]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIIPALLRRDDGLLLLTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=867570 Q9K10_RS21750 WP_060422991.1 4494867..4495889(-) (pilT) [Serratia marcescens strain GES6]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGGCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACCGGCCGGGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCATCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGCTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATATTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATTGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCACCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Neisseria gonorrhoeae MS11

49.851

98.529

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Pseudomonas aeruginosa PAK

49.258

99.118

0.488
  pilT Pseudomonas stutzeri DSM 10701

48.665

99.118

0.482
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.258

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371