Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q8X47_RS04130 Genome accession   NZ_CP132061
Coordinates   862595..863575 (+) Length   326 a.a.
NCBI ID   WP_086977593.1    Uniprot ID   -
Organism   Escherichia coli strain QML220614     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 857595..868575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q8X47_RS04100 - 858902..859147 (+) 246 Protein_810 DUF1202 family protein -
  Q8X47_RS04105 hemW 859272..860408 (-) 1137 WP_000239953.1 radical SAM family heme chaperone HemW -
  Q8X47_RS04110 rdgB 860401..860994 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  Q8X47_RS04115 yggU 861002..861292 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  Q8X47_RS04120 yggT 861289..861855 (-) 567 WP_001094820.1 osmotic shock tolerance protein YggT -
  Q8X47_RS04125 yggS 861873..862577 (-) 705 WP_000997804.1 pyridoxal phosphate homeostasis protein -
  Q8X47_RS04130 pilT 862595..863575 (+) 981 WP_086977593.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q8X47_RS04135 ruvX 863749..864165 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  Q8X47_RS04140 yqgE 864165..864728 (-) 564 WP_001301052.1 YqgE/AlgH family protein -
  Q8X47_RS04145 gshB 864837..865787 (-) 951 WP_000593260.1 glutathione synthase -
  Q8X47_RS04150 rsmE 865800..866531 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q8X47_RS04155 endA 866611..867318 (-) 708 WP_000286492.1 deoxyribonuclease I -
  Q8X47_RS04160 yggI 867413..867910 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35985.23 Da        Isoelectric Point: 6.1764

>NTDB_id=867082 Q8X47_RS04130 WP_086977593.1 862595..863575(+) (pilT) [Escherichia coli strain QML220614]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSKNGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEEPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=867082 Q8X47_RS04130 WP_086977593.1 862595..863575(+) (pilT) [Escherichia coli strain QML220614]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCAAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGAACCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365