Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q6H37_RS23180 Genome accession   NZ_CP131467
Coordinates   5023361..5024416 (-) Length   351 a.a.
NCBI ID   WP_318736930.1    Uniprot ID   -
Organism   Clostridium sp. JS66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5018361..5029416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q6H37_RS23160 (Q6H37_23165) ftsZ 5018874..5020004 (-) 1131 WP_318736923.1 cell division protein FtsZ -
  Q6H37_RS23165 (Q6H37_23170) ftsA 5020020..5021291 (-) 1272 WP_318736924.1 cell division protein FtsA -
  Q6H37_RS23170 (Q6H37_23175) - 5021466..5022740 (-) 1275 WP_318736926.1 glycosyltransferase family 2 protein -
  Q6H37_RS23175 (Q6H37_23180) - 5023163..5023321 (+) 159 WP_318736928.1 hypothetical protein -
  Q6H37_RS23180 (Q6H37_23185) pilT 5023361..5024416 (-) 1056 WP_318736930.1 type IV pilus twitching motility protein PilT Machinery gene
  Q6H37_RS23185 (Q6H37_23190) sigK 5024660..5025361 (-) 702 WP_007063576.1 RNA polymerase sporulation sigma factor SigK -
  Q6H37_RS23190 (Q6H37_23195) - 5025495..5027153 (-) 1659 WP_318736932.1 penicillin-binding transpeptidase domain-containing protein -
  Q6H37_RS23195 (Q6H37_23200) udk 5027214..5027840 (-) 627 WP_007063578.1 uridine kinase -
  Q6H37_RS23200 (Q6H37_23205) - 5027868..5029088 (-) 1221 WP_318736933.1 U32 family peptidase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38693.73 Da        Isoelectric Point: 7.1612

>NTDB_id=862506 Q6H37_RS23180 WP_318736930.1 5023361..5024416(-) (pilT) [Clostridium sp. JS66]
MISLKELLEITIKNNASDLHLTVGVPPIIRINGELSTVNSEKLQPADTESYSKEILNEDYEEYIKKGELDTSFSVSGLGR
FRINLFKQRGSCSLAIRVVGLKIPSIGDLNFPSVIKEITNNKRGLVLVTGPTGSGKSTTLAAMINEINCTRAAHIITLED
PIEYLHKHNKSIINQREIGKDSLSYHNALKAVLREDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTIGASKTIDRI
VDVFPPYQQQQVKTQLAAVLKGIISQQLLPKVDGNGRIASLEIMIATAAIQNMIREGKTHQIESSIQTGAKYGMKTMDMS
LAELYKRGMISYECALSYAMDRDILSRIISL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=862506 Q6H37_RS23180 WP_318736930.1 5023361..5024416(-) (pilT) [Clostridium sp. JS66]
GTGATATCACTAAAGGAATTATTGGAAATAACCATTAAAAATAATGCATCTGATTTACATTTAACTGTAGGAGTTCCACC
AATAATAAGAATAAATGGAGAATTATCAACTGTTAATAGTGAAAAACTTCAACCAGCAGATACGGAAAGTTATAGTAAAG
AAATTTTGAATGAAGACTACGAAGAATATATTAAAAAAGGTGAATTGGATACTTCTTTTTCTGTATCGGGATTAGGAAGA
TTTAGAATTAATTTGTTTAAGCAAAGAGGAAGTTGTTCACTAGCTATAAGGGTTGTAGGACTAAAGATACCTTCAATAGG
TGATTTGAATTTTCCATCTGTAATAAAAGAAATTACAAATAACAAAAGAGGTCTTGTATTAGTTACAGGTCCTACTGGAT
CAGGGAAAAGTACTACATTAGCGGCAATGATAAATGAAATAAATTGTACTAGGGCAGCACATATTATTACTCTTGAAGAT
CCAATTGAATATTTACATAAACATAATAAATCTATAATAAACCAAAGGGAAATAGGAAAAGATAGCTTGAGTTATCATAA
TGCTTTAAAAGCGGTTTTAAGAGAAGATCCAGATGTTATTCTTGTGGGAGAAATGAGGGATTTGGAAACTATTTCTATAG
CACTTACAGCTGCTGAAACAGGGCACTTGGTATTTTCTACGTTACATACAATAGGTGCATCGAAAACTATTGATAGAATA
GTTGATGTATTTCCTCCATATCAACAGCAGCAAGTAAAAACACAATTAGCAGCAGTCCTTAAAGGGATTATATCACAACA
ACTGTTGCCTAAAGTTGATGGTAATGGAAGAATAGCAAGTTTAGAAATAATGATAGCAACTGCAGCTATTCAAAATATGA
TAAGAGAAGGAAAAACTCATCAAATAGAATCTTCTATACAAACAGGAGCTAAATATGGTATGAAAACTATGGATATGTCT
TTAGCTGAGTTATACAAAAGAGGAATGATATCATATGAATGTGCTTTGAGCTATGCTATGGATAGGGATATACTAAGCAG
AATAATATCATTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

50.877

97.436

0.496
  pilT Acinetobacter baumannii strain A118

50.877

97.436

0.496
  pilT Acinetobacter baumannii D1279779

50.877

97.436

0.496
  pilT Acinetobacter baylyi ADP1

50.585

97.436

0.493
  pilT Neisseria meningitidis 8013

49.856

98.86

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.952

94.872

0.493
  pilT Vibrio cholerae strain A1552

51.952

94.872

0.493
  pilT Neisseria gonorrhoeae MS11

49.422

98.575

0.487
  pilT Legionella pneumophila strain ERS1305867

48.851

99.145

0.484
  pilT Legionella pneumophila strain Lp02

48.851

99.145

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.851

99.145

0.484
  pilT Pseudomonas stutzeri DSM 10701

50.296

96.296

0.484
  pilT Pseudomonas aeruginosa PAK

49.415

97.436

0.481
  pilU Pseudomonas stutzeri DSM 10701

41.311

100

0.413
  pilU Acinetobacter baylyi ADP1

41.159

98.291

0.405
  pilU Vibrio cholerae strain A1552

39.349

96.296

0.379