Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q5Z10_RS02665 Genome accession   NZ_CP130831
Coordinates   582612..583739 (-) Length   375 a.a.
NCBI ID   WP_303637809.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 704A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 577612..588739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5Z10_RS02645 - 578020..579009 (+) 990 WP_303637805.1 fumarylacetoacetate hydrolase family protein -
  Q5Z10_RS02650 maiA 579024..579692 (+) 669 WP_303637806.1 maleylacetoacetate isomerase -
  Q5Z10_RS02655 - 579721..581217 (-) 1497 WP_303637807.1 UdgX family uracil-DNA binding protein -
  Q5Z10_RS02660 - 581198..582481 (-) 1284 WP_303637808.1 putative DNA modification/repair radical SAM protein -
  Q5Z10_RS02665 pilU 582612..583739 (-) 1128 WP_303637809.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5Z10_RS02670 - 583861..584226 (+) 366 WP_303637810.1 DUF4398 domain-containing protein -
  Q5Z10_RS02675 - 584223..585107 (+) 885 WP_303637811.1 hypothetical protein -
  Q5Z10_RS02680 - 585432..585668 (+) 237 WP_079220527.1 YdcH family protein -
  Q5Z10_RS02685 - 585982..587352 (+) 1371 WP_303637812.1 pyridoxal-phosphate dependent enzyme -
  Q5Z10_RS02690 - 587476..588657 (+) 1182 WP_303637813.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41442.71 Da        Isoelectric Point: 6.3738

>NTDB_id=861467 Q5Z10_RS02665 WP_303637809.1 582612..583739(-) (pilU) [Stenotrophomonas sp. 704A1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKNGTIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSAGQPTITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=861467 Q5Z10_RS02665 WP_303637809.1 582612..583739(-) (pilU) [Stenotrophomonas sp. 704A1]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTATCCGCTCGGCAACACCGGCCTGCCGCCCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAACGCGACCTGGAGCTCAACATGGCCATCGCCCTGGCAGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTACTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTGGGGCTGGACACCCATGCGTTCCACAA
TGCGCTGAAGAACGCGATGCGCGAGGCGCCGGATGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACGATGGAGGCAG
CCATCGCCTTCGCCGAGACCGGGCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCACTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGGCGCCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGGCAGGTGCACGAGATCAAGGCAGCGATGGAGGCGTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGTTGGCCAAGAACGGCACCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGTTGAA
GTTCCGGTTGTCCGAGGGCGGCAGCGGCGAGCATGACCCGTACGCCGACTTCTCGGCCGGGCAGCCGACGATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Vibrio cholerae strain A1552

49.167

96

0.472
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.512

91.733

0.427
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.541

88.8

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

88.8

0.36
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36