Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WCU32_RS04240 Genome accession   NZ_CP147574
Coordinates   864066..865046 (+) Length   326 a.a.
NCBI ID   WP_001546096.1    Uniprot ID   -
Organism   Escherichia coli strain GN03590     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 859066..870046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU32_RS04210 (WCU32_04210) yggM 859667..860674 (+) 1008 WP_102778638.1 DUF1202 family protein -
  WCU32_RS04215 (WCU32_04215) hemW 860743..861879 (-) 1137 WP_000239978.1 radical SAM family heme chaperone HemW -
  WCU32_RS04220 (WCU32_04220) rdgB 861872..862465 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  WCU32_RS04225 (WCU32_04225) yggU 862473..862763 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  WCU32_RS04230 (WCU32_04230) yggT 862760..863326 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  WCU32_RS04235 (WCU32_04235) yggS 863344..864048 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  WCU32_RS04240 (WCU32_04240) pilT 864066..865046 (+) 981 WP_001546096.1 type IV pilus twitching motility protein PilT Machinery gene
  WCU32_RS04245 (WCU32_04245) ruvX 865225..865641 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  WCU32_RS04250 (WCU32_04250) yqgE 865641..866204 (-) 564 WP_001355636.1 YqgE/AlgH family protein -
  WCU32_RS04255 (WCU32_04255) gshB 866313..867263 (-) 951 WP_000593260.1 glutathione synthase -
  WCU32_RS04260 (WCU32_04260) rsmE 867276..868007 (-) 732 WP_001531929.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WCU32_RS04265 (WCU32_04265) endA 868087..868794 (-) 708 WP_001305312.1 deoxyribonuclease I -
  WCU32_RS04270 (WCU32_04270) yggI 868889..869386 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35944.14 Da        Isoelectric Point: 5.7980

>NTDB_id=861308 WCU32_RS04240 WP_001546096.1 864066..865046(+) (pilT) [Escherichia coli strain GN03590]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLLHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=861308 WCU32_RS04240 WP_001546096.1 864066..865046(+) (pilT) [Escherichia coli strain GN03590]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGCTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACTGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365