Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q1J09_RS03690 Genome accession   NZ_CP130618
Coordinates   770471..771451 (+) Length   326 a.a.
NCBI ID   WP_045901825.1    Uniprot ID   A0A5T3MD10
Organism   Salmonella enterica subsp. enterica serovar Abortusovis strain 401964R1_S49     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765471..776451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q1J09_RS03660 (Q1J09_03665) - 766048..767055 (+) 1008 WP_023209131.1 DUF1202 domain-containing protein -
  Q1J09_RS03665 (Q1J09_03670) hemW 767147..768283 (-) 1137 WP_023207107.1 radical SAM family heme chaperone HemW -
  Q1J09_RS03670 (Q1J09_03675) - 768276..768869 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  Q1J09_RS03675 (Q1J09_03680) yggU 768877..769167 (-) 291 WP_322499620.1 DUF167 family protein YggU -
  Q1J09_RS03680 (Q1J09_03685) - 769164..769730 (-) 567 WP_001094848.1 YggT family protein -
  Q1J09_RS03685 (Q1J09_03690) - 769749..770453 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q1J09_RS03690 (Q1J09_03695) pilT 770471..771451 (+) 981 WP_045901825.1 type IV pilus twitching motility protein PilT Machinery gene
  Q1J09_RS03695 (Q1J09_03700) - 771583..772269 (+) 687 WP_058108548.1 IclR family transcriptional regulator -
  Q1J09_RS03700 (Q1J09_03705) ruvX 772316..772732 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  Q1J09_RS03705 (Q1J09_03710) - 772732..773295 (-) 564 WP_322499621.1 YqgE/AlgH family protein -
  Q1J09_RS03710 (Q1J09_03715) gshB 773511..774458 (-) 948 WP_322499622.1 glutathione synthase -
  Q1J09_RS03715 (Q1J09_03720) rsmE 774478..775209 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q1J09_RS03720 (Q1J09_03725) endA 775286..775993 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36137.38 Da        Isoelectric Point: 8.0973

>NTDB_id=860689 Q1J09_RS03690 WP_045901825.1 770471..771451(+) (pilT) [Salmonella enterica subsp. enterica serovar Abortusovis strain 401964R1_S49]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=860689 Q1J09_RS03690 WP_045901825.1 770471..771451(+) (pilT) [Salmonella enterica subsp. enterica serovar Abortusovis strain 401964R1_S49]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTGAAGATCCTGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5T3MD10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362