Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WAZ53_RS22300 Genome accession   NZ_CP147053
Coordinates   4434876..4435856 (-) Length   326 a.a.
NCBI ID   WP_050483793.1    Uniprot ID   -
Organism   Escherichia coli strain 1008S13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4429876..4440856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ53_RS22265 (WAZ53_22265) yggI 4430531..4431028 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  WAZ53_RS22270 (WAZ53_22270) endA 4431123..4431830 (+) 708 WP_000286500.1 deoxyribonuclease I -
  WAZ53_RS22275 (WAZ53_22275) rsmE 4431910..4432641 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WAZ53_RS22280 (WAZ53_22280) gshB 4432654..4433604 (+) 951 WP_000593273.1 glutathione synthase -
  WAZ53_RS22285 (WAZ53_22285) yqgE 4433713..4434276 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  WAZ53_RS22290 (WAZ53_22290) ruvX 4434276..4434692 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  WAZ53_RS22295 (WAZ53_22295) - 4434699..4434827 (-) 129 WP_001278270.1 hypothetical protein -
  WAZ53_RS22300 (WAZ53_22300) pilT 4434876..4435856 (-) 981 WP_050483793.1 type IV pilus twitching motility protein PilT Machinery gene
  WAZ53_RS22305 (WAZ53_22305) yggS 4435874..4436578 (+) 705 WP_000997804.1 pyridoxal phosphate homeostasis protein -
  WAZ53_RS22310 (WAZ53_22310) yggT 4436596..4437162 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  WAZ53_RS22315 (WAZ53_22315) yggU 4437159..4437449 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  WAZ53_RS22320 (WAZ53_22320) rdgB 4437457..4438050 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  WAZ53_RS22325 (WAZ53_22325) hemW 4438043..4439179 (+) 1137 WP_000239953.1 radical SAM family heme chaperone HemW -
  WAZ53_RS22330 (WAZ53_22330) yggM 4439304..4440251 (-) 948 WP_341853448.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35981.16 Da        Isoelectric Point: 5.9549

>NTDB_id=858199 WAZ53_RS22300 WP_050483793.1 4434876..4435856(-) (pilT) [Escherichia coli strain 1008S13]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=858199 WAZ53_RS22300 WP_050483793.1 4434876..4435856(-) (pilT) [Escherichia coli strain 1008S13]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

46.226

97.546

0.451
  pilT Legionella pneumophila strain Lp02

46.226

97.546

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.373

98.773

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.667

100

0.371