Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WAZ59_RS20170 Genome accession   NZ_CP147032
Coordinates   4030659..4031639 (+) Length   326 a.a.
NCBI ID   WP_341857110.1    Uniprot ID   -
Organism   Escherichia coli strain 22F46     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4025659..4036639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ59_RS20140 (WAZ59_20140) yggM 4026174..4027181 (+) 1008 WP_000745217.1 DUF1202 family protein -
  WAZ59_RS20145 (WAZ59_20145) hemW 4027336..4028472 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  WAZ59_RS20150 (WAZ59_20150) rdgB 4028465..4029058 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  WAZ59_RS20155 (WAZ59_20155) yggU 4029066..4029356 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  WAZ59_RS20160 (WAZ59_20160) yggT 4029353..4029919 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  WAZ59_RS20165 (WAZ59_20165) yggS 4029937..4030641 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  WAZ59_RS20170 (WAZ59_20170) pilT 4030659..4031639 (+) 981 WP_341857110.1 type IV pilus twitching motility protein PilT Machinery gene
  WAZ59_RS20175 (WAZ59_20175) ruvX 4031823..4032239 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  WAZ59_RS20180 (WAZ59_20180) yqgE 4032239..4032802 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  WAZ59_RS20185 (WAZ59_20185) gshB 4032911..4033861 (-) 951 WP_000593273.1 glutathione synthase -
  WAZ59_RS20190 (WAZ59_20190) rsmE 4033874..4034605 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WAZ59_RS20195 (WAZ59_20195) endA 4034685..4035392 (-) 708 WP_000286500.1 deoxyribonuclease I -
  WAZ59_RS20200 (WAZ59_20200) yggI 4035487..4035984 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35966.15 Da        Isoelectric Point: 6.2279

>NTDB_id=857698 WAZ59_RS20170 WP_341857110.1 4030659..4031639(+) (pilT) [Escherichia coli strain 22F46]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENSLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREGPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=857698 WAZ59_RS20170 WP_341857110.1 4030659..4031639(+) (pilT) [Escherichia coli strain 22F46]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATAGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGGTCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.283

97.546

0.442
  pilT Legionella pneumophila strain Lp02

45.283

97.546

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39
  pilU Acinetobacter baylyi ADP1

35.758

100

0.362