Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QZL36_RS13180 Genome accession   NZ_CP129932
Coordinates   2863663..2864769 (-) Length   368 a.a.
NCBI ID   WP_308385403.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus isolate BM18B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2858663..2869769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZL36_RS13150 - 2859318..2859815 (+) 498 WP_015297290.1 SprT family zinc-dependent metalloprotease -
  QZL36_RS13155 endA 2859935..2860630 (+) 696 WP_005493878.1 deoxyribonuclease I -
  QZL36_RS13160 rsmE 2860755..2861486 (+) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QZL36_RS13165 gshB 2861501..2862451 (+) 951 WP_005425823.1 glutathione synthase -
  QZL36_RS13170 - 2862565..2863128 (+) 564 WP_005461717.1 YqgE/AlgH family protein -
  QZL36_RS13175 ruvX 2863177..2863602 (+) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  QZL36_RS13180 pilU 2863663..2864769 (-) 1107 WP_308385403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QZL36_RS13185 pilT 2864800..2865840 (-) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  QZL36_RS13190 - 2865869..2866582 (+) 714 WP_021450047.1 YggS family pyridoxal phosphate-dependent enzyme -
  QZL36_RS13195 proC 2866702..2867520 (+) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  QZL36_RS13200 - 2867573..2868130 (+) 558 WP_005482440.1 YggT family protein -
  QZL36_RS13205 yggU 2868130..2868420 (+) 291 WP_005482461.1 DUF167 family protein YggU -
  QZL36_RS13210 - 2868548..2868979 (+) 432 WP_005461703.1 DUF4426 domain-containing protein -
  QZL36_RS13215 - 2869097..2869699 (+) 603 WP_005482459.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41484.76 Da        Isoelectric Point: 7.2517

>NTDB_id=856447 QZL36_RS13180 WP_308385403.1 2863663..2864769(-) (pilU) [Vibrio parahaemolyticus isolate BM18B]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKIREEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=856447 QZL36_RS13180 WP_308385403.1 2863663..2864769(-) (pilU) [Vibrio parahaemolyticus isolate BM18B]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAGCATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
GTTAGTGCTTTTTTTCAGCGAGAACTGCCGGGGGCAGTTATTCGCCGTATCGAAACCATCATTCCTACTTTTGAGCAGTT
AAAACTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCGACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGTTTGGATACCGAAAGCTATGAAGTCGCACT
GAAAAACTCGTTGCGCCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAGCGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGACGTCATGGTGTGTTTGAGATATTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAATCAACCATGGCACGATCCAATGAGTTTGGCATGCTGACGTTTGACCAATCGCTATAC
AAGCTGGTGATGCAGGGTAAGATCAGAGAAGAGGATGCACTGCACAGTGCGGATTCAGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

42.121

89.674

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.674

0.375
  pilT Vibrio cholerae strain A1552

41.818

89.674

0.375
  pilT Neisseria gonorrhoeae MS11

40.361

90.217

0.364
  pilT Neisseria meningitidis 8013

40.361

90.217

0.364