Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q0M94_RS05350 Genome accession   NZ_CP129906
Coordinates   1013443..1014630 (+) Length   395 a.a.
NCBI ID   WP_407540819.1    Uniprot ID   -
Organism   Deinococcus radiomollis strain PO-04-20-132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1008443..1019630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0M94_RS05335 (Q0M94_05335) - 1009110..1009946 (+) 837 WP_407540816.1 polyphenol oxidase family protein -
  Q0M94_RS05340 (Q0M94_05340) - 1009943..1010638 (+) 696 WP_407540817.1 YqeG family HAD IIIA-type phosphatase -
  Q0M94_RS05345 (Q0M94_05345) pilB 1010638..1013304 (+) 2667 WP_407540818.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  Q0M94_RS05350 (Q0M94_05350) pilT 1013443..1014630 (+) 1188 WP_407540819.1 type IV pilus twitching motility protein PilT Machinery gene
  Q0M94_RS05355 (Q0M94_05355) - 1014730..1014939 (+) 210 WP_407540820.1 hypothetical protein -
  Q0M94_RS05360 (Q0M94_05360) - 1015678..1016175 (-) 498 WP_407540821.1 hypothetical protein -
  Q0M94_RS05365 (Q0M94_05365) - 1017034..1018932 (+) 1899 WP_407540822.1 HD domain-containing phosphohydrolase -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 43020.33 Da        Isoelectric Point: 7.2034

>NTDB_id=856348 Q0M94_RS05350 WP_407540819.1 1013443..1014630(+) (pilT) [Deinococcus radiomollis strain PO-04-20-132]
MHVATDITDILRVAAEKGASDVILTVGMPPQFKIHGVYGGQDFSELSATDTRKLMYSMMNEKQQRTFEEKRELDFSFALG
EKARFRVNTFMQKGNVGGVMRLIPTIIKSVSDLGLPPNVVDVANAPRGLVLVTGPTGSGKSTTLAAMLDYINVNKKMHIM
TIEDPIEFMHTHKNSIVNQREVGSDTTDFQSALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPES
IDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRIDGGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMITM
DAYLAGLFRKRLITHETGMERAVDSKEFSRLASDPAAANALSPTLSGSMGMGGAPARPTASTTPASPSGNNYGRR

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=856348 Q0M94_RS05350 WP_407540819.1 1013443..1014630(+) (pilT) [Deinococcus radiomollis strain PO-04-20-132]
ATGCATGTAGCCACCGACATCACCGACATCCTGCGGGTCGCCGCCGAGAAGGGCGCCAGCGACGTGATTTTGACCGTCGG
CATGCCGCCGCAGTTCAAGATTCACGGCGTCTACGGCGGTCAGGATTTTTCCGAGCTGAGCGCCACCGACACCCGCAAGC
TGATGTACAGCATGATGAACGAGAAGCAGCAGCGCACCTTCGAGGAGAAGCGCGAACTCGACTTCTCGTTCGCGCTCGGT
GAGAAGGCCCGCTTCCGCGTGAACACCTTCATGCAGAAGGGCAACGTGGGCGGCGTGATGCGCCTGATTCCCACCATCAT
CAAGTCGGTGAGCGACCTGGGCCTGCCGCCCAACGTGGTGGACGTCGCCAACGCGCCGCGCGGACTGGTGCTCGTAACCG
GGCCGACCGGCTCCGGTAAATCGACCACCCTGGCGGCGATGCTCGACTACATCAACGTCAACAAGAAGATGCACATCATG
ACCATCGAAGACCCGATCGAGTTCATGCATACCCACAAGAACAGCATCGTCAACCAGCGCGAGGTGGGGTCCGACACCAC
CGATTTCCAGAGTGCGCTGCGCGCGGTGCTGCGCCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGCGATTACGAGA
CCATCAAAGCCGCCGTGACCGCCGCCGAGACCGGCCACCTGGTGATGGGCACCCTGCACACCAACAGCGCCCCGGAGAGC
ATCGACCGCATCGTGGACGTGTTCCCGGAAGAGCAGCAGGAGCAGATCCGGGTGCAGCTCGCCAACAACCTGGTGGCCGT
CATGACCCAGCAGCTGCTGCCGCGCATCGACGGCGGGCGTATTCTGGCCTACGAGCTGCTGATCGCCAACCCGGCGGTGC
GGGCGCTGATCCGCGAGGGTAAAACGTACCAGATCACATCGGTCATGCAGACCGGGGCGCGCGAGGGCATGATCACCATG
GACGCGTACCTCGCGGGGCTGTTCCGCAAGCGCCTGATCACCCACGAGACCGGCATGGAACGCGCCGTGGACAGCAAGGA
GTTTTCACGCCTGGCCTCCGACCCGGCGGCGGCGAACGCCCTCAGCCCCACCCTGAGCGGCAGCATGGGCATGGGCGGCG
CGCCCGCCCGGCCGACCGCCAGCACCACCCCGGCCTCACCCAGCGGCAACAACTACGGTCGGCGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

75.315

100

0.757
  pilT Pseudomonas aeruginosa PAK

51.603

86.835

0.448
  pilT Acinetobacter baumannii D1279779

51.603

86.835

0.448
  pilT Acinetobacter baumannii strain A118

51.603

86.835

0.448
  pilT Acinetobacter nosocomialis M2

51.603

86.835

0.448
  pilT Neisseria meningitidis 8013

51.163

87.089

0.446
  pilT Pseudomonas stutzeri DSM 10701

51.312

86.835

0.446
  pilT Neisseria gonorrhoeae MS11

50.872

87.089

0.443
  pilT Acinetobacter baylyi ADP1

50.729

86.835

0.441
  pilT Legionella pneumophila strain Lp02

49.271

86.835

0.428
  pilT Legionella pneumophila strain ERS1305867

49.271

86.835

0.428
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.316

80

0.403
  pilT Vibrio cholerae strain A1552

50.316

80

0.403
  pilU Pseudomonas stutzeri DSM 10701

44.412

86.076

0.382
  pilU Vibrio cholerae strain A1552

43.413

84.557

0.367