Detailed information NTG0856347

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: Q0M94_RS05345
• Genome accession: NZ_CP129906
• Coordinates: 1010638..1013304 (+)
• Length: 888 a.a.
• NCBI ID: WP_407540818.1
• Uniprot ID: -
• Organism: Deinococcus radiomollis strain PO-04-20-132
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1005638..1018304

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q0M94_RS05320 (Q0M94_05320)	-	1006765..1007433 (+)	669	WP_407540813.1	response regulator transcription factor	-
Q0M94_RS05325 (Q0M94_05325)	-	1007570..1008160 (+)	591	WP_407540814.1	CAP domain-containing protein	-
Q0M94_RS05330 (Q0M94_05330)	-	1008201..1008938 (-)	738	WP_407540815.1	metallophosphoesterase family protein	-
Q0M94_RS05335 (Q0M94_05335)	-	1009110..1009946 (+)	837	WP_407540816.1	polyphenol oxidase family protein	-
Q0M94_RS05340 (Q0M94_05340)	-	1009943..1010638 (+)	696	WP_407540817.1	YqeG family HAD IIIA-type phosphatase	-
Q0M94_RS05345 (Q0M94_05345)	pilB	1010638..1013304 (+)	2667	WP_407540818.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
Q0M94_RS05350 (Q0M94_05350)	pilT	1013443..1014630 (+)	1188	WP_407540819.1	type IV pilus twitching motility protein PilT	Machinery gene
Q0M94_RS05355 (Q0M94_05355)	-	1014730..1014939 (+)	210	WP_407540820.1	hypothetical protein	-
Q0M94_RS05360 (Q0M94_05360)	-	1015678..1016175 (-)	498	WP_407540821.1	hypothetical protein	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 96844.06 Da
• Isoelectric Point: 5.0286

>NTDB_id=856347 Q0M94_RS05345 WP_407540818.1 1010638..1013304(+) (pilB) [Deinococcus radiomollis strain PO-04-20-132]
MALSIGDRRLGAILLEQGYVNDIDLQKALNKHAEVGGRLADILIDGGMVGEKRIARAVEEALGIPLVNLTVLSPDAQALA
SVKAQVALSALAFPFALEGDTLRVAFVDPLNNLNLETVEDASGLNVELYQALREQVQWAIASNYPELGLNVNMPMEPQDG
TVSLLGQRLISRGLISESALLETLEQQKQSGEPLGSLLIGLKLLSEEQLYETLAEQSGTVFVRNPRDYEPAEDVLGMLLR
ADALRLSAVPIEEKEGSITVVGSDPRRLGDIESLVGKPVHLLLGRPSDVEALIDRCYPQRGRLGEAMVQQGSLSRAQLRE
ALQVQARTGKVKPLGEVIVDLGFAGSEEIDAALQKQTSGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPDP
KVALLIPESTARRYVVVPMRLQGNSLVVAMKDPRNVFALDDLRLLTGRDIVPAVMAEKDIVRLIERYFGSQDMVKLNEEL
VRDSRGRDKEREAQQDQTNLDDNAVVRVVDSIIREAALQEASDIHIEPGEFSMKVRYRIDGTLREHMELPKGSAQSVLAR
IKILGQLDIAERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGLSEHNFQRFTDLIEKPNG
IFLITGPTGSGKSFSSFSILKRIARPEKNTTTIEDPVEYEIPGINQSQVNPVAGMTFARALRAFLRQDPDIIFVGEIRDA
ETAKIATEAALTGHLVLATLHTNDAPGAVTRLEEMGVEHFNIGAALIGVLGQRLVRKVCPECKTPTNADPDVLRRLGINE
KELKGANLVRGAGCPRCGGTGYKGRMGIHELMIIDDHLRRGIGKGEGANELRDIATTQSGMKTLRQDGIEKAMAGVTTLE
EVLAVTSI

Nucleotide

• Length: 2667 bp

>NTDB_id=856347 Q0M94_RS05345 WP_407540818.1 1010638..1013304(+) (pilB) [Deinococcus radiomollis strain PO-04-20-132]
ATGGCACTTTCAATTGGAGACCGGCGGCTGGGAGCGATCCTGCTGGAACAGGGCTACGTCAACGACATCGATCTGCAGAA
GGCGCTCAACAAGCACGCCGAGGTGGGTGGCCGTCTGGCCGACATCCTGATCGACGGCGGCATGGTGGGCGAGAAGCGGA
TTGCCAGAGCCGTCGAGGAGGCGCTGGGCATTCCGCTCGTCAACCTGACGGTGCTCAGCCCCGACGCTCAGGCGCTCGCC
AGCGTCAAGGCGCAGGTGGCGCTCTCGGCGCTGGCCTTCCCGTTCGCGCTGGAGGGCGACACGTTGCGGGTGGCTTTCGT
CGATCCGCTGAACAACCTGAACCTGGAAACCGTGGAAGACGCCAGCGGCCTGAACGTCGAGCTGTATCAGGCGCTGCGCG
AGCAGGTGCAGTGGGCGATTGCCAGCAACTACCCGGAACTGGGCCTGAACGTGAACATGCCGATGGAGCCGCAGGACGGC
ACGGTGTCGCTGCTCGGCCAGCGTCTGATTTCACGCGGATTGATTTCCGAATCGGCGCTGCTGGAAACGCTCGAACAGCA
GAAGCAGAGTGGTGAGCCGCTGGGCTCGCTCCTGATCGGGCTGAAACTGCTGTCGGAAGAGCAGCTCTACGAGACGCTGG
CCGAACAGTCGGGCACCGTCTTCGTGCGTAACCCGCGTGACTACGAACCGGCCGAAGACGTGCTCGGCATGCTGCTGCGC
GCCGACGCGCTGCGGCTGTCGGCGGTGCCCATCGAGGAGAAGGAAGGCAGCATCACGGTGGTGGGCAGCGACCCGCGCCG
CCTGGGCGATATCGAGTCGCTGGTGGGCAAGCCTGTTCATCTGCTGCTGGGCCGCCCGAGCGACGTCGAAGCCCTGATCG
ACCGCTGCTACCCGCAACGTGGACGGCTGGGCGAGGCGATGGTGCAGCAGGGATCGCTGTCGCGCGCCCAGCTGCGAGAG
GCGCTTCAGGTGCAGGCCCGCACCGGCAAGGTCAAGCCGCTCGGTGAGGTGATCGTGGACCTGGGCTTCGCCGGCAGCGA
GGAGATCGACGCCGCGCTCCAGAAGCAGACCTCCGGTGGCGGACGGCTGGAAGACACGCTGGTGCAGTCGGGCAAGCTCA
GCCCCGAGATGCTGGCGCGCAGCCTCGCGGCCCAGCTCGGCTACGAATTTCTCGACCCGGTGCAGAATCCGCCCGACCCC
AAGGTGGCGCTGCTCATTCCCGAGAGCACCGCCCGCCGCTACGTGGTGGTGCCCATGCGCCTGCAGGGCAACTCGCTGGT
GGTCGCCATGAAGGACCCGCGCAACGTGTTCGCGCTCGACGACCTGCGCCTGCTGACGGGCCGCGACATCGTGCCGGCGG
TGATGGCCGAAAAAGACATCGTGCGCCTGATCGAGCGGTATTTCGGCTCGCAGGACATGGTGAAGCTGAACGAGGAACTG
GTGCGCGACAGCCGGGGCCGCGACAAGGAGCGCGAGGCCCAGCAGGACCAGACCAACCTCGACGACAACGCGGTGGTGCG
GGTGGTGGACAGCATCATCCGGGAAGCGGCGCTTCAGGAGGCCAGCGACATTCACATCGAGCCGGGCGAGTTCAGCATGA
AGGTGCGCTACCGCATCGACGGCACGCTGCGCGAGCACATGGAGCTGCCGAAAGGTTCGGCCCAGAGCGTGCTGGCGCGC
ATCAAGATTCTCGGCCAGCTCGACATCGCCGAACGCCGGGTGCCGCAGGACGGCCGCGTGCGCTTCAAGAAGGGCAGCAT
CGACCTCGACCTGCGACTGAGCACCCTGCCCACGGTCTACGGTGAGAAGGCCGTGATGCGCCTCTTGCAGAAGGCCAGCA
ACATTCCTGAAGTCGAGCAGCTCGGCCTGAGCGAACACAACTTTCAGCGCTTCACCGACCTGATCGAGAAGCCCAACGGT
ATTTTCCTGATCACCGGACCGACCGGCTCGGGCAAGTCGTTTTCCAGCTTTTCTATCCTCAAACGCATCGCCAGGCCCGA
GAAGAACACCACCACCATCGAGGACCCGGTCGAATACGAGATTCCCGGCATCAACCAGTCGCAGGTGAACCCGGTGGCGG
GCATGACGTTTGCCAGAGCACTCAGAGCGTTCCTGCGGCAGGACCCCGACATCATCTTCGTCGGCGAGATTCGAGACGCC
GAGACCGCCAAGATCGCCACCGAGGCGGCCCTCACCGGCCACCTGGTGCTGGCGACCCTGCACACCAACGACGCGCCCGG
AGCGGTGACCCGCCTCGAAGAGATGGGCGTGGAGCACTTCAACATCGGCGCGGCATTGATCGGGGTGCTGGGCCAGCGGC
TGGTGCGCAAGGTCTGCCCGGAGTGCAAGACGCCCACCAACGCCGACCCCGACGTGCTGCGGCGGCTGGGCATCAATGAG
AAGGAACTGAAAGGGGCCAACCTGGTGCGCGGAGCCGGCTGCCCGCGCTGCGGCGGCACCGGCTACAAGGGCCGGATGGG
CATTCACGAGCTGATGATCATCGACGACCACCTGCGGCGCGGCATCGGCAAGGGCGAGGGCGCCAACGAGCTGCGCGACA
TCGCCACCACCCAGAGCGGCATGAAGACGCTGCGCCAGGACGGCATCGAGAAGGCGATGGCGGGCGTCACCACGCTGGAA
GAAGTGCTGGCCGTGACCAGCATCTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (198-301)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (367-472)

• Type II/IV secretion system protein (T2SSE) (512-777)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	72.022	100	0.722
pilF	Thermus thermophilus HB27	55.668	100	0.559