Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V8956_RS02040 Genome accession   NZ_CP146571
Coordinates   332657..333637 (-) Length   326 a.a.
NCBI ID   WP_001055632.1    Uniprot ID   -
Organism   Escherichia coli strain B1.H1.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327657..338637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8956_RS02010 (V8956_02010) yggI 328283..328780 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  V8956_RS02015 (V8956_02015) endA 328875..329582 (+) 708 WP_000286500.1 deoxyribonuclease I -
  V8956_RS02020 (V8956_02020) rsmE 329662..330393 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V8956_RS02025 (V8956_02025) gshB 330406..331356 (+) 951 WP_000593273.1 glutathione synthase -
  V8956_RS02030 (V8956_02030) yqgE 331465..332028 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  V8956_RS02035 (V8956_02035) ruvX 332028..332444 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  V8956_RS02040 (V8956_02040) pilT 332657..333637 (-) 981 WP_001055632.1 type IV pilus twitching motility protein PilT Machinery gene
  V8956_RS02045 (V8956_02045) yggS 333655..334359 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  V8956_RS02050 (V8956_02050) yggT 334377..334943 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  V8956_RS02055 (V8956_02055) yggU 334940..335230 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  V8956_RS02060 (V8956_02060) rdgB 335238..335831 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  V8956_RS02065 (V8956_02065) hemW 335824..336960 (+) 1137 WP_000239986.1 radical SAM family heme chaperone HemW -
  V8956_RS02070 (V8956_02070) - 337273..338259 (+) 987 WP_001530835.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36000.25 Da        Isoelectric Point: 5.7980

>NTDB_id=855599 V8956_RS02040 WP_001055632.1 332657..333637(-) (pilT) [Escherichia coli strain B1.H1.1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAVPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPLILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=855599 V8956_RS02040 WP_001055632.1 332657..333637(-) (pilT) [Escherichia coli strain B1.H1.1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGTGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACTGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365