Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R2TS_RS18830 Genome accession   NZ_AP024498
Coordinates   3929205..3930185 (-) Length   326 a.a.
NCBI ID   WP_182071655.1    Uniprot ID   -
Organism   Enterobacter asburiae strain En30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3924205..3935185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2TS_RS18800 (R2TS_36780) - 3925081..3925578 (+) 498 WP_033146604.1 SprT family zinc-dependent metalloprotease -
  R2TS_RS18805 (R2TS_36790) endA 3925673..3926380 (+) 708 WP_023333343.1 deoxyribonuclease I -
  R2TS_RS18810 (R2TS_36800) rsmE 3926432..3927163 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R2TS_RS18815 (R2TS_36810) gshB 3927183..3928130 (+) 948 WP_029739222.1 glutathione synthase -
  R2TS_RS18820 (R2TS_36820) - 3928218..3928778 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  R2TS_RS18825 (R2TS_36830) ruvX 3928778..3929194 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  R2TS_RS18830 (R2TS_36840) pilT 3929205..3930185 (-) 981 WP_182071655.1 type IV pilus twitching motility protein PilT Machinery gene
  R2TS_RS18835 (R2TS_36850) - 3930203..3930904 (+) 702 WP_182071654.1 YggS family pyridoxal phosphate-dependent enzyme -
  R2TS_RS18840 (R2TS_36860) - 3930926..3931492 (+) 567 WP_047060424.1 YggT family protein -
  R2TS_RS18845 (R2TS_36870) yggU 3931489..3931785 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  R2TS_RS18850 (R2TS_36880) - 3931789..3932382 (+) 594 WP_048979374.1 XTP/dITP diphosphatase -
  R2TS_RS18855 (R2TS_36890) hemW 3932375..3933511 (+) 1137 WP_182071653.1 radical SAM family heme chaperone HemW -
  R2TS_RS18860 (R2TS_36900) - 3933581..3933919 (+) 339 WP_039262962.1 endonuclease domain-containing protein -
  R2TS_RS18865 (R2TS_36910) - 3934002..3934718 (-) 717 WP_024906551.1 DUF2884 domain-containing protein -
  R2TS_RS18870 (R2TS_36920) - 3934775..3935101 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35427.45 Da        Isoelectric Point: 6.6088

>NTDB_id=85388 R2TS_RS18830 WP_182071655.1 3929205..3930185(-) (pilT) [Enterobacter asburiae strain En30]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMSGISVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQPSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=85388 R2TS_RS18830 WP_182071655.1 3929205..3930185(-) (pilT) [Enterobacter asburiae strain En30]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGCCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGTCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGACAGCCCAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAAAGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGCGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.61

100

0.509
  pilT Vibrio cholerae strain A1552

50.61

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment