Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QOM25_RS10005 Genome accession   NZ_CP129497
Coordinates   2012114..2013094 (+) Length   326 a.a.
NCBI ID   WP_301512714.1    Uniprot ID   -
Organism   Enterobacter asburiae strain MP4m4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2007114..2018094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOM25_RS09965 (QOM25_09965) - 2007181..2007507 (+) 327 WP_014885198.1 YggL family protein -
  QOM25_RS09970 (QOM25_09970) - 2007564..2008280 (+) 717 WP_033146612.1 DUF2884 domain-containing protein -
  QOM25_RS09975 (QOM25_09975) - 2008356..2008718 (-) 363 WP_301512710.1 endonuclease domain-containing protein -
  QOM25_RS09980 (QOM25_09980) hemW 2008782..2009924 (-) 1143 WP_301512711.1 radical SAM family heme chaperone HemW -
  QOM25_RS09985 (QOM25_09985) - 2009917..2010510 (-) 594 WP_032660047.1 XTP/dITP diphosphatase -
  QOM25_RS09990 (QOM25_09990) yggU 2010514..2010810 (-) 297 WP_301512712.1 DUF167 family protein YggU -
  QOM25_RS09995 (QOM25_09995) - 2010807..2011373 (-) 567 WP_024906554.1 YggT family protein -
  QOM25_RS10000 (QOM25_10000) - 2011395..2012096 (-) 702 WP_301512713.1 YggS family pyridoxal phosphate-dependent enzyme -
  QOM25_RS10005 (QOM25_10005) pilT 2012114..2013094 (+) 981 WP_301512714.1 type IV pilus twitching motility protein PilT Machinery gene
  QOM25_RS10010 (QOM25_10010) ruvX 2013105..2013521 (-) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  QOM25_RS10015 (QOM25_10015) - 2013521..2014081 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  QOM25_RS10020 (QOM25_10020) gshB 2014169..2015116 (-) 948 WP_032660059.1 glutathione synthase -
  QOM25_RS10025 (QOM25_10025) rsmE 2015136..2015867 (-) 732 WP_063439817.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QOM25_RS10030 (QOM25_10030) endA 2015919..2016626 (-) 708 WP_301512715.1 deoxyribonuclease I -
  QOM25_RS10035 (QOM25_10035) - 2016721..2017218 (-) 498 WP_202773643.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35521.53 Da        Isoelectric Point: 6.9108

>NTDB_id=853637 QOM25_RS10005 WP_301512714.1 2012114..2013094(+) (pilT) [Enterobacter asburiae strain MP4m4]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATATGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RTQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=853637 QOM25_RS10005 WP_301512714.1 2012114..2013094(+) (pilT) [Enterobacter asburiae strain MP4m4]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAGCCCGCTCCCTTTCCGCCTCCCGATGTCGGAGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGCCAGGTGGATTTTGCCGCGACGGCGACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTCAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTTAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTGATTCTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGCGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGACGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.097

97.853

0.5
  pilT Vibrio cholerae strain A1552

51.097

97.853

0.5
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Pseudomonas stutzeri DSM 10701

49.062

98.16

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Acinetobacter baumannii D1279779

48.438

98.16

0.475
  pilT Acinetobacter baumannii strain A118

48.438

98.16

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

48.125

98.16

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.405
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362