Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TBR22_RS18850 Genome accession   NZ_AP024452
Coordinates   4502919..4504046 (-) Length   375 a.a.
NCBI ID   WP_239489388.1    Uniprot ID   A0A915UJ16
Organism   Luteitalea sp. TBR-22     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4497919..4509046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TBR22_RS18835 (TBR22_37610) - 4498382..4499812 (-) 1431 WP_239489385.1 sigma-54 dependent transcriptional regulator -
  TBR22_RS18840 (TBR22_37620) - 4499839..4501539 (-) 1701 WP_239489386.1 nitrogen regulation protein NR(II) -
  TBR22_RS18845 (TBR22_37630) - 4501540..4502748 (-) 1209 WP_239489387.1 type II secretion system F family protein -
  TBR22_RS18850 (TBR22_37640) pilT 4502919..4504046 (-) 1128 WP_239489388.1 type IV pilus twitching motility protein PilT Machinery gene
  TBR22_RS18855 (TBR22_37650) pilB 4504466..4506214 (-) 1749 WP_239489389.1 type IV-A pilus assembly ATPase PilB -
  TBR22_RS18860 (TBR22_37660) traF 4506344..4507348 (-) 1005 WP_239489390.1 conjugal transfer protein TraF -
  TBR22_RS18865 (TBR22_37670) - 4507489..4508214 (+) 726 WP_239489391.1 YIP1 family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41346.69 Da        Isoelectric Point: 8.5003

>NTDB_id=85153 TBR22_RS18850 WP_239489388.1 4502919..4504046(-) (pilT) [Luteitalea sp. TBR-22]
MATLPELLKTTVELDGSDLHLTTSAPPTVRVHGKLVPLKLPALTAPETKQLAYSVCTDAQKKRFEETQELDFSFGIRGVG
RFRCNIFNQRGAVASVYRLIPEKVRGFQELNLPPILARLSERPRGLVLVTGPTGSGKSTTLAAMIDKINTERNEHILTIE
DPIEYIHQHKGCLVNQREVHSDTQSFSGALRAALREDPDVVLIGEMRDLETIESALRIAETGHLTFATLHTNSASQTINR
IIDVFPAHQQSQIRTQLSLVLEGIVCQSLLRRANGPGRVVSLEILVPTPAIRNLIRDDKVHQIYSMMQAGQEKLGMQTMN
QSLASLYMARQITLETAMAASSMKEELQEMIQRGSGVVPGAGLNRPGMPGVPVRR

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=85153 TBR22_RS18850 WP_239489388.1 4502919..4504046(-) (pilT) [Luteitalea sp. TBR-22]
ATGGCGACACTGCCCGAACTGCTGAAGACGACGGTGGAGCTCGACGGCTCGGACCTCCACCTCACCACGAGCGCCCCGCC
GACCGTGCGCGTGCACGGCAAGCTGGTGCCCCTGAAGCTGCCGGCGCTCACGGCGCCCGAGACCAAGCAGCTGGCCTACA
GCGTCTGCACCGACGCCCAGAAGAAGCGCTTCGAGGAGACGCAGGAACTCGACTTCTCGTTCGGCATCCGCGGCGTCGGC
CGGTTCCGCTGCAACATCTTCAACCAGCGCGGCGCCGTCGCCTCGGTGTATCGCCTGATTCCGGAGAAGGTCCGCGGCTT
CCAGGAACTCAACCTGCCGCCGATCCTGGCCAGGCTGTCGGAGCGGCCGCGCGGCCTCGTGCTGGTCACCGGTCCCACCG
GCAGCGGCAAGTCGACGACGCTGGCGGCGATGATCGACAAGATCAACACCGAGCGCAACGAGCACATCCTCACCATCGAG
GACCCGATCGAGTACATCCACCAGCACAAGGGCTGCCTGGTGAACCAGCGCGAGGTCCACAGCGACACGCAGAGCTTCAG
CGGCGCGTTGCGCGCCGCGCTCCGCGAGGACCCCGACGTGGTGCTGATCGGCGAGATGCGCGACCTCGAGACCATCGAGT
CGGCGCTGCGGATCGCCGAGACCGGTCACCTCACGTTCGCGACGCTGCACACCAATTCCGCGTCGCAGACCATCAACCGC
ATCATCGACGTGTTCCCCGCGCACCAGCAGTCGCAGATCCGCACGCAGCTGTCGCTCGTGCTCGAGGGCATCGTGTGCCA
GTCGCTGCTGCGCCGGGCCAACGGGCCGGGCCGCGTCGTGTCGCTCGAGATCCTCGTCCCGACGCCGGCAATCCGGAACC
TGATTCGCGACGACAAGGTGCACCAGATCTACTCGATGATGCAGGCCGGGCAGGAGAAGCTCGGCATGCAGACGATGAAC
CAGTCGCTGGCGTCGCTGTACATGGCGCGGCAGATCACGCTGGAAACGGCGATGGCGGCCTCCTCGATGAAGGAAGAACT
GCAGGAGATGATCCAGCGGGGGAGCGGCGTGGTCCCCGGCGCGGGCCTGAACCGGCCGGGGATGCCCGGCGTGCCGGTGC
GGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

55.325

90.133

0.499
  pilT Legionella pneumophila strain ERS1305867

55.325

90.133

0.499
  pilT Acinetobacter baylyi ADP1

52.326

91.733

0.48
  pilT Pseudomonas aeruginosa PAK

52.326

91.733

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.649

98.667

0.48
  pilT Acinetobacter baumannii strain A118

52.035

91.733

0.477
  pilT Acinetobacter baumannii D1279779

52.035

91.733

0.477
  pilT Acinetobacter nosocomialis M2

52.035

91.733

0.477
  pilT Pseudomonas stutzeri DSM 10701

52.994

89.067

0.472
  pilT Neisseria meningitidis 8013

52.941

86.133

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.632

86.133

0.453
  pilT Vibrio cholerae strain A1552

52.632

86.133

0.453
  pilT Neisseria gonorrhoeae MS11

52.632

86.133

0.453
  pilU Pseudomonas stutzeri DSM 10701

43.75

93.867

0.411
  pilU Vibrio cholerae strain A1552

42.939

92.533

0.397
  pilU Acinetobacter baylyi ADP1

41.617

89.067

0.371

Multiple sequence alignment