Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QVH37_RS19155 Genome accession   NZ_CP129026
Coordinates   4003807..4004787 (-) Length   326 a.a.
NCBI ID   WP_267217302.1    Uniprot ID   -
Organism   Enterobacter pseudoroggenkampii strain FY158     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3998807..4009787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QVH37_RS19125 (QVH37_19125) - 3999683..4000180 (+) 498 WP_032650144.1 SprT family zinc-dependent metalloprotease -
  QVH37_RS19130 (QVH37_19130) endA 4000275..4000982 (+) 708 WP_023333343.1 deoxyribonuclease I -
  QVH37_RS19135 (QVH37_19135) rsmE 4001034..4001765 (+) 732 WP_024906558.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QVH37_RS19140 (QVH37_19140) gshB 4001785..4002732 (+) 948 WP_157189705.1 glutathione synthase -
  QVH37_RS19145 (QVH37_19145) - 4002820..4003380 (+) 561 WP_032645077.1 YqgE/AlgH family protein -
  QVH37_RS19150 (QVH37_19150) ruvX 4003380..4003796 (+) 417 WP_235402081.1 Holliday junction resolvase RuvX -
  QVH37_RS19155 (QVH37_19155) pilT 4003807..4004787 (-) 981 WP_267217302.1 type IV pilus twitching motility protein PilT Machinery gene
  QVH37_RS19160 (QVH37_19160) - 4004805..4005506 (+) 702 WP_248272396.1 YggS family pyridoxal phosphate-dependent enzyme -
  QVH37_RS19165 (QVH37_19165) - 4005528..4006094 (+) 567 WP_090418613.1 YggT family protein -
  QVH37_RS19170 (QVH37_19170) yggU 4006091..4006387 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  QVH37_RS19175 (QVH37_19175) - 4006391..4006984 (+) 594 WP_024906553.1 XTP/dITP diphosphatase -
  QVH37_RS19180 (QVH37_19180) hemW 4006977..4008119 (+) 1143 WP_290093921.1 radical SAM family heme chaperone HemW -
  QVH37_RS19185 (QVH37_19185) - 4008183..4008521 (+) 339 WP_267215590.1 endonuclease domain-containing protein -
  QVH37_RS19190 (QVH37_19190) - 4008604..4009320 (-) 717 WP_024906551.1 DUF2884 domain-containing protein -
  QVH37_RS19195 (QVH37_19195) - 4009377..4009703 (-) 327 WP_290093922.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35422.52 Da        Isoelectric Point: 6.8407

>NTDB_id=850716 QVH37_RS19155 WP_267217302.1 4003807..4004787(-) (pilT) [Enterobacter pseudoroggenkampii strain FY158]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAIGQVDFAVTVAGGQRLRG
SAFKQMSGISVTLRLLPRTCPQLASLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNRHADGHILTLEDPVE
FIYRSERCLIQQREIGQHSPSFAEALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=850716 QVH37_RS19155 WP_267217302.1 4003807..4004787(-) (pilT) [Enterobacter pseudoroggenkampii strain FY158]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCTGACGTCGGGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGGATTTGGTGGGCCATCGGGCAGGTGGATTTTGCCGTGACGGTGGCGGGAGGCCAGCGTCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTAACGCTCCGGCTGTTGCCGCGTACCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTGTCCAATGACGCCGGACTGATTCTGGTGACAGGAGCAACCGGCAGCGGTAAAT
CAACTACGCTGGCGGCGATGGTCGATTTTCTTAACCGCCATGCTGATGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCGGAGCGAGCGTTGTCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAAGCGCTGCG
CGGTGCCTTACGCCAGGATCCGGACGTGATTCTGCTTGGGGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAACACCGCAGCGGCGGCGAATCTCATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGCATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362