Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QVH39_RS19285 Genome accession   NZ_CP129025
Coordinates   4030090..4031070 (-) Length   326 a.a.
NCBI ID   WP_061714497.1    Uniprot ID   -
Organism   Enterobacter pseudoroggenkampii strain SC1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4025090..4036070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QVH39_RS19255 (QVH39_19255) - 4025966..4026463 (+) 498 WP_032650144.1 SprT family zinc-dependent metalloprotease -
  QVH39_RS19260 (QVH39_19260) endA 4026558..4027265 (+) 708 WP_023333343.1 deoxyribonuclease I -
  QVH39_RS19265 (QVH39_19265) rsmE 4027317..4028048 (+) 732 WP_024906558.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QVH39_RS19270 (QVH39_19270) gshB 4028068..4029015 (+) 948 WP_061714498.1 glutathione synthase -
  QVH39_RS19275 (QVH39_19275) - 4029103..4029663 (+) 561 WP_032645077.1 YqgE/AlgH family protein -
  QVH39_RS19280 (QVH39_19280) ruvX 4029663..4030079 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  QVH39_RS19285 (QVH39_19285) pilT 4030090..4031070 (-) 981 WP_061714497.1 type IV pilus twitching motility protein PilT Machinery gene
  QVH39_RS19290 (QVH39_19290) - 4031088..4031789 (+) 702 WP_061714496.1 YggS family pyridoxal phosphate-dependent enzyme -
  QVH39_RS19295 (QVH39_19295) - 4031811..4032377 (+) 567 WP_024906554.1 YggT family protein -
  QVH39_RS19300 (QVH39_19300) yggU 4032374..4032670 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  QVH39_RS19305 (QVH39_19305) - 4032674..4033267 (+) 594 WP_048991669.1 XTP/dITP diphosphatase -
  QVH39_RS19310 (QVH39_19310) hemW 4033260..4034402 (+) 1143 WP_061714494.1 radical SAM family heme chaperone HemW -
  QVH39_RS19315 (QVH39_19315) - 4034466..4034828 (+) 363 WP_073961472.1 endonuclease domain-containing protein -
  QVH39_RS19320 (QVH39_19320) - 4034890..4035606 (-) 717 WP_024906551.1 DUF2884 domain-containing protein -
  QVH39_RS19325 (QVH39_19325) - 4035663..4035989 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35396.44 Da        Isoelectric Point: 6.8407

>NTDB_id=850693 QVH39_RS19285 WP_061714497.1 4030090..4031070(-) (pilT) [Enterobacter pseudoroggenkampii strain SC1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGIWWASGQVDFAVTVAGGQRLRG
SAFKQMSGISVTLRLLPRTCPQLASLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNRHADGHILTLEDPVE
FIYRSERCLIQQREIGQHSPSFAEALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=850693 QVH39_RS19285 WP_061714497.1 4030090..4031070(-) (pilT) [Enterobacter pseudoroggenkampii strain SC1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAGCCGGCACCGTTTCCTCCTCCCGACGTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGGATATGGTGGGCCAGCGGGCAGGTGGATTTTGCCGTGACGGTGGCGGGAGGCCAGCGTCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTAACGCTCCGGCTGTTGCCGCGTACCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTGTCCAATGACGCCGGACTGATTCTGGTGACAGGAGCAACCGGCAGCGGTAAAT
CAACTACGCTGGCGGCGATGGTCGATTTTCTTAACCGCCATGCTGATGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCGGAGCGAACGTTGTCTGATCCAGCAGCGGGAGATAGGTCAGCACAGCCCGTCATTTGCAGAAGCGCTGCG
CGGTGCCTTACGCCAGGATCCGGACGTGATTCTGCTTGGGGAGTTGCGCGACAGCGAAACGATCCGTCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGCTGTATGAACTGCTGGTGAACACCGCAGCGGCGGCGAATCTCATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATTATTCAAACCGGTCAGCAGGCAGGTATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362