Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VXE09_RS04040 Genome accession   NZ_CP144710
Coordinates   832662..833642 (+) Length   326 a.a.
NCBI ID   WP_020239575.1    Uniprot ID   -
Organism   Escherichia coli strain B18271     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 827662..838642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXE09_RS04010 (VXE09_04010) yggM 828177..829184 (+) 1008 WP_000745217.1 DUF1202 family protein -
  VXE09_RS04015 (VXE09_04015) hemW 829339..830475 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  VXE09_RS04020 (VXE09_04020) rdgB 830468..831061 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  VXE09_RS04025 (VXE09_04025) yggU 831069..831359 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  VXE09_RS04030 (VXE09_04030) yggT 831356..831922 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  VXE09_RS04035 (VXE09_04035) yggS 831940..832644 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  VXE09_RS04040 (VXE09_04040) pilT 832662..833642 (+) 981 WP_020239575.1 type IV pilus twitching motility protein PilT Machinery gene
  VXE09_RS04045 (VXE09_04045) ruvX 833826..834242 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  VXE09_RS04050 (VXE09_04050) yqgE 834242..834805 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  VXE09_RS04055 (VXE09_04055) gshB 834914..835864 (-) 951 WP_000593273.1 glutathione synthase -
  VXE09_RS04060 (VXE09_04060) rsmE 835877..836608 (-) 732 WP_251265460.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VXE09_RS04065 (VXE09_04065) endA 836688..837395 (-) 708 WP_000286500.1 deoxyribonuclease I -
  VXE09_RS04070 (VXE09_04070) yggI 837490..837987 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35936.12 Da        Isoelectric Point: 6.2279

>NTDB_id=847439 VXE09_RS04040 WP_020239575.1 832662..833642(+) (pilT) [Escherichia coli strain B18271]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREGPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=847439 VXE09_RS04040 WP_020239575.1 832662..833642(+) (pilT) [Escherichia coli strain B18271]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGGTCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.373

98.773

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393
  pilU Acinetobacter baylyi ADP1

36.364

100

0.368