Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V4942_RS07220 Genome accession   NZ_CP144687
Coordinates   1342021..1343127 (-) Length   368 a.a.
NCBI ID   WP_011173796.1    Uniprot ID   Q72HS1
Organism   Thermus thermophilus strain TTHB27_delta_cmr4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1337021..1348127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4942_RS07195 (V4942_07195) - 1337707..1338078 (-) 372 WP_011173792.1 polymer-forming cytoskeletal protein -
  V4942_RS07200 (V4942_07200) - 1338080..1338979 (-) 900 WP_011173793.1 M23 family metallopeptidase -
  V4942_RS07205 (V4942_07205) - 1339109..1340380 (-) 1272 WP_011173794.1 thymidine phosphorylase -
  V4942_RS07210 (V4942_07210) - 1340403..1340921 (+) 519 WP_041443572.1 Uma2 family endonuclease -
  V4942_RS07215 (V4942_07215) fba 1340986..1341903 (-) 918 WP_008633565.1 class II fructose-1,6-bisphosphate aldolase -
  V4942_RS07220 (V4942_07220) pilT 1342021..1343127 (-) 1107 WP_011173796.1 type IV pilus twitching motility protein PilT Machinery gene
  V4942_RS07225 (V4942_07225) panC 1343124..1343954 (-) 831 WP_011173797.1 pantoate--beta-alanine ligase -
  V4942_RS07230 (V4942_07230) - 1343976..1344254 (+) 279 WP_008633571.1 hypothetical protein -
  V4942_RS07235 (V4942_07235) rsfS 1344312..1344653 (-) 342 WP_011173798.1 ribosome silencing factor -
  V4942_RS07240 (V4942_07240) - 1344660..1345757 (-) 1098 WP_011173799.1 LCP family protein -
  V4942_RS07245 (V4942_07245) yqeK 1345757..1346302 (-) 546 WP_011173800.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V4942_RS07250 (V4942_07250) nadD 1346277..1346837 (-) 561 WP_011173801.1 nicotinate-nucleotide adenylyltransferase -
  V4942_RS07255 (V4942_07255) obgE 1346838..1348088 (-) 1251 WP_011173802.1 GTPase ObgE -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41108.68 Da        Isoelectric Point: 6.5817

>NTDB_id=847402 V4942_RS07220 WP_011173796.1 1342021..1343127(-) (pilT) [Thermus thermophilus strain TTHB27_delta_cmr4]
MSEAQGQGKQSLVEMLKAMVQARASDIHLQAGAPPTVRIDGKLRPFGNRPLTPKEVEAIARALLTPEQLEELEYRKEMDF
AYTIPGVARFRCNLLRQRGSFGLVMRVVSEVIPSFEALGLPREVMEGLAAKERGLILVTGPTGSGKSTTLAALIDHINLH
YAKNIITIEDPIEFLHKHKKSLVVQREVGLDTDSFYTGLKYALRQDPDVIMVGEMRDRETVEAALMAAQTGHLVLSTLHT
LDAWRTINRIIDFFPLHEHRQVRILLAESLLGILSQRLLPKADGQGRVLALEILIATPYVRELLKDEEKTPQIKEAMMEG
ALYGMRTFDQHLVELYTEGLISLEDALSAATSPHEFRLLLTKATGQTY

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=847402 V4942_RS07220 WP_011173796.1 1342021..1343127(-) (pilT) [Thermus thermophilus strain TTHB27_delta_cmr4]
ATGAGCGAGGCCCAAGGCCAGGGCAAGCAGAGCCTCGTGGAGATGCTGAAGGCCATGGTCCAGGCCCGGGCCTCGGACAT
CCACCTGCAGGCCGGGGCCCCGCCCACCGTCCGGATAGACGGCAAGCTCCGCCCCTTCGGCAACCGCCCCTTGACCCCCA
AGGAGGTGGAGGCCATCGCCCGGGCCCTCCTCACCCCGGAACAGCTGGAGGAGCTGGAGTACCGCAAGGAGATGGACTTC
GCCTACACCATCCCCGGGGTGGCCCGCTTCCGCTGCAACCTCCTGAGGCAACGGGGGAGCTTCGGCCTGGTGATGCGGGT
GGTCTCCGAGGTGATCCCGAGCTTTGAGGCCCTGGGCCTCCCCCGGGAGGTGATGGAGGGCCTCGCCGCCAAGGAGCGGG
GCCTCATTCTGGTCACGGGGCCCACGGGGAGCGGCAAGAGCACCACCCTGGCGGCCCTCATTGACCACATCAACCTCCAC
TACGCCAAGAACATCATCACCATTGAGGACCCCATTGAGTTCCTGCACAAGCACAAGAAGAGCCTCGTGGTCCAGCGGGA
GGTGGGGCTGGACACGGACAGCTTCTACACCGGCCTCAAGTACGCCCTCCGCCAGGACCCCGACGTGATCATGGTGGGGG
AGATGCGGGACCGGGAGACGGTGGAGGCCGCCCTCATGGCGGCGCAGACGGGCCACCTGGTCCTCTCCACCCTCCACACC
CTGGACGCCTGGCGGACCATCAACCGGATCATTGACTTCTTTCCCCTGCACGAGCACCGTCAGGTGCGCATCCTCCTCGC
CGAGTCCCTCCTCGGCATCCTCTCCCAGCGCCTCCTCCCCAAGGCCGACGGCCAGGGCCGGGTCCTCGCCCTGGAGATCC
TCATCGCCACGCCCTACGTGCGGGAGCTTCTCAAGGACGAGGAGAAGACGCCCCAGATCAAGGAGGCCATGATGGAGGGG
GCCCTCTACGGGATGCGCACCTTTGACCAGCACCTGGTGGAGCTCTACACCGAGGGGCTCATCTCCCTGGAGGACGCCCT
CTCCGCCGCCACCAGCCCCCACGAGTTCCGGCTCCTCCTCACCAAGGCCACGGGGCAGACCTACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(16-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72HS1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.025

95.924

0.451
  pilT Legionella pneumophila strain ERS1305867

47.826

93.75

0.448
  pilT Legionella pneumophila strain Lp02

47.826

93.75

0.448
  pilT Pseudomonas aeruginosa PAK

47.246

93.75

0.443
  pilT Acinetobacter baylyi ADP1

46.957

93.75

0.44
  pilT Acinetobacter baumannii strain A118

46.377

93.75

0.435
  pilT Acinetobacter nosocomialis M2

46.377

93.75

0.435
  pilT Acinetobacter baumannii D1279779

46.377

93.75

0.435
  pilT Pseudomonas stutzeri DSM 10701

46.087

93.75

0.432
  pilT Vibrio cholerae strain A1552

46.291

91.576

0.424
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.291

91.576

0.424
  pilT Neisseria gonorrhoeae MS11

45.075

91.033

0.41
  pilT Neisseria meningitidis 8013

45.075

91.033

0.41
  pilU Pseudomonas stutzeri DSM 10701

42.363

94.293

0.399
  pilU Acinetobacter baylyi ADP1

42.197

94.022

0.397
  pilU Vibrio cholerae strain A1552

40.896

97.011

0.397