Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QR290_RS27685 Genome accession   NZ_CP128260
Coordinates   6144899..6145933 (-) Length   344 a.a.
NCBI ID   WP_007953348.1    Uniprot ID   J3FB13
Organism   Pseudomonas fluorescens strain PH.SM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6139899..6150933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR290_RS27655 (QR290_27655) ruvX 6140191..6140628 (+) 438 WP_007953334.1 Holliday junction resolvase RuvX -
  QR290_RS27660 (QR290_27660) pyrR 6140710..6141213 (+) 504 WP_007953335.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QR290_RS27665 (QR290_27665) - 6141241..6142245 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  QR290_RS27670 (QR290_27670) - 6142242..6143513 (+) 1272 WP_085746690.1 dihydroorotase -
  QR290_RS27675 (QR290_27675) - 6143549..6143983 (-) 435 WP_115079505.1 TM2 domain-containing protein -
  QR290_RS27680 (QR290_27680) - 6144233..6144850 (+) 618 WP_007953345.1 C40 family peptidase -
  QR290_RS27685 (QR290_27685) pilT 6144899..6145933 (-) 1035 WP_007953348.1 type IV pilus twitching motility protein PilT Machinery gene
  QR290_RS27690 (QR290_27690) - 6146128..6146814 (+) 687 WP_289203994.1 YggS family pyridoxal phosphate-dependent enzyme -
  QR290_RS27695 (QR290_27695) proC 6146838..6147656 (+) 819 WP_115079507.1 pyrroline-5-carboxylate reductase -
  QR290_RS27700 (QR290_27700) - 6147667..6148257 (+) 591 WP_011336374.1 YggT family protein -
  QR290_RS27705 (QR290_27705) - 6148254..6148544 (+) 291 WP_289203995.1 DUF167 domain-containing protein -
  QR290_RS27710 (QR290_27710) - 6148610..6149749 (+) 1140 WP_115079509.1 homoserine O-acetyltransferase -
  QR290_RS27715 (QR290_27715) metW 6149757..6150377 (+) 621 WP_115079510.1 methionine biosynthesis protein MetW -
  QR290_RS27720 (QR290_27720) - 6150404..6150838 (+) 435 WP_007953365.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0157

>NTDB_id=846933 QR290_RS27685 WP_007953348.1 6144899..6145933(-) (pilT) [Pseudomonas fluorescens strain PH.SM]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=846933 QR290_RS27685 WP_007953348.1 6144899..6145933(-) (pilT) [Pseudomonas fluorescens strain PH.SM]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTGGATGGCGACGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAATGCCTTCAACCAGAACCGGGGTGCCGGTGCGGTTTTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTTGCCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCACGCGGGCTGGTGCTGGTGACCGGACCGACCGGCT
CCGGCAAGTCCACGACGCTGGCGGCGATGATCGATTACCTAAACACTCACCGTCATCACCACATCCTGACCATCGAAGAT
CCGATCGAATTCGTCCACGAATCACGCAAATGCCTGATCAATCAACGTGAAGTCCACCGCGATACCCGCAGTTTCGCCAC
CGCGTTGCGTTCAGCGCTGCGCGAAGATCCGGATGTGATCCTGGTGGGCGAGATGCGTGATCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCCGAAACCGGGCACCTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGGGGACGAAAAGTCCATGGTGCGCTCGATGCTGTCGGAGTCGTTGCTGGCCGTAGTCTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCGCACGAGATCATGCTGGGGACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATACAGACCGGCGGCTCGCTGGGGATGCAGACACTCGACATGTGCCTG
AAGGATTTGGTGACCAAGGGCTTGATCAGCCGCGAACATGCGCGGGAAAAGGCGCGCACGCCGGATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FB13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398