Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R1N_RS19775 Genome accession   NZ_AP024281
Coordinates   4078345..4079325 (-) Length   326 a.a.
NCBI ID   WP_033146606.1    Uniprot ID   -
Organism   Enterobacter asburiae strain Ent261     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4073345..4084325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1N_RS19745 (R1N_38740) - 4074221..4074718 (+) 498 WP_033146604.1 SprT family zinc-dependent metalloprotease -
  R1N_RS19750 (R1N_38750) endA 4074813..4075520 (+) 708 WP_023333343.1 deoxyribonuclease I -
  R1N_RS19755 (R1N_38760) rsmE 4075572..4076303 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R1N_RS19760 (R1N_38770) gshB 4076323..4077270 (+) 948 WP_033146605.1 glutathione synthase -
  R1N_RS19765 (R1N_38780) - 4077358..4077918 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  R1N_RS19770 (R1N_38790) ruvX 4077918..4078334 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  R1N_RS19775 (R1N_38800) pilT 4078345..4079325 (-) 981 WP_033146606.1 type IV pilus twitching motility protein PilT Machinery gene
  R1N_RS19780 (R1N_38810) - 4079343..4080044 (+) 702 WP_033146607.1 YggS family pyridoxal phosphate-dependent enzyme -
  R1N_RS19785 (R1N_38820) - 4080066..4080632 (+) 567 WP_033146608.1 YggT family protein -
  R1N_RS19790 (R1N_38830) yggU 4080629..4080925 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  R1N_RS19795 (R1N_38840) - 4080929..4081522 (+) 594 WP_033146609.1 XTP/dITP diphosphatase -
  R1N_RS19800 (R1N_38850) hemW 4081515..4082657 (+) 1143 WP_033146610.1 radical SAM family heme chaperone HemW -
  R1N_RS19805 (R1N_38860) - 4082721..4083083 (+) 363 WP_071993605.1 endonuclease domain-containing protein -
  R1N_RS19810 (R1N_38870) - 4083145..4083861 (-) 717 WP_033146612.1 DUF2884 domain-containing protein -
  R1N_RS19815 (R1N_38880) - 4083918..4084244 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35548.56 Da        Isoelectric Point: 6.9701

>NTDB_id=84524 R1N_RS19775 WP_033146606.1 4078345..4079325(-) (pilT) [Enterobacter asburiae strain Ent261]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFHPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLSSLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=84524 R1N_RS19775 WP_033146606.1 4078345..4079325(-) (pilT) [Enterobacter asburiae strain Ent261]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCACCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAACTCTCTTCGCTGGG
CGCGCCGCGGGTAATCCCGGAGCTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGTCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGTGCCTTGCGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGACTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAGACCGGTCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

49.39

100

0.497
  pilT Acinetobacter baumannii D1279779

48.78

100

0.491
  pilT Acinetobacter baumannii strain A118

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

48.476

100

0.488
  pilT Pseudomonas aeruginosa PAK

48.318

100

0.485
  pilT Acinetobacter nosocomialis M2

49.221

98.466

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment