Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QSG86_RS01165 Genome accession   NZ_CP127915
Coordinates   16886..17923 (+) Length   345 a.a.
NCBI ID   WP_317032489.1    Uniprot ID   -
Organism   Acinetobacter sp. SAAs474     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 11886..22923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG86_RS01155 (QSG86_01150) - 12344..15943 (+) 3600 WP_317032487.1 AAA family ATPase -
  QSG86_RS01160 (QSG86_01155) - 16063..16761 (-) 699 WP_317032488.1 YggS family pyridoxal phosphate-dependent enzyme -
  QSG86_RS01165 (QSG86_01160) pilT 16886..17923 (+) 1038 WP_317032489.1 type IV pilus twitching motility protein PilT Machinery gene
  QSG86_RS01170 (QSG86_01165) fur 18042..18479 (-) 438 WP_317032490.1 ferric iron uptake transcriptional regulator -
  QSG86_RS01175 (QSG86_01170) - 18591..18989 (+) 399 WP_317032491.1 outer membrane protein assembly factor BamE -
  QSG86_RS01180 (QSG86_01175) - 19037..19357 (-) 321 WP_317032492.1 RnfH family protein -
  QSG86_RS01185 (QSG86_01180) - 19354..20439 (-) 1086 WP_317032493.1 hypothetical protein -
  QSG86_RS01190 (QSG86_01185) - 20711..21178 (-) 468 WP_317032494.1 bacteriohemerythrin -
  QSG86_RS01195 (QSG86_01190) - 21681..22529 (-) 849 WP_317032495.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38620.66 Da        Isoelectric Point: 6.8172

>NTDB_id=845195 QSG86_RS01165 WP_317032489.1 16886..17923(+) (pilT) [Acinetobacter sp. SAAs474]
MDITALLTYAVQHGASDLHLSAGMPPIVRIDGELQPMDVSCLDHEQIQQLILSIMDQQQGHNFITEREIDFAFELPKVSR
FRINAFHQNRGASAVFRIISSQILSMEQLALPAIFQQICEYPRGIVLVTGPTGSGKSTTLAAMIDYINHHYCKHILSIED
PIEFIHQSKKCLINQREIYRDTLGLNQALHAALREDPDIILIGEMRDAETMRLALTAAETGHLVFATLHTSSATKTIDRI
IDTFSAQEKDSVRAMLAESLQAVISQILLKKQHQGRVAAHEIMIAIPAIRNLIRENKIAQMYSVLQTGAVYGMQTLDQSL
KHLLHQGTITLQTARSLAKQPEMFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=845195 QSG86_RS01165 WP_317032489.1 16886..17923(+) (pilT) [Acinetobacter sp. SAAs474]
ATGGATATTACCGCTTTATTAACTTACGCTGTGCAACATGGCGCATCTGATTTACATTTGTCGGCAGGTATGCCACCAAT
TGTTCGTATTGATGGCGAACTACAGCCTATGGATGTATCTTGTTTAGATCATGAGCAAATACAACAATTAATTTTAAGTA
TTATGGATCAGCAACAAGGCCATAATTTTATCACAGAACGAGAAATTGATTTTGCTTTTGAACTGCCCAAGGTATCGCGT
TTTCGTATTAATGCATTTCATCAAAATCGTGGTGCTAGCGCAGTATTCCGTATCATTTCATCACAAATACTCAGTATGGA
ACAACTGGCATTACCAGCAATTTTTCAACAAATTTGTGAATATCCACGTGGTATTGTTTTAGTCACCGGCCCAACAGGAT
CTGGTAAATCAACGACCTTGGCTGCGATGATTGATTATATTAACCATCATTATTGCAAACATATTCTCAGCATTGAAGAT
CCTATAGAATTTATTCATCAATCAAAAAAATGCTTAATTAATCAGCGTGAGATATATCGTGATACATTAGGTTTAAATCA
AGCATTACATGCCGCACTACGTGAAGATCCCGATATTATTTTGATTGGTGAAATGCGCGATGCGGAAACGATGCGATTGG
CTTTAACTGCAGCAGAAACAGGACATTTAGTTTTTGCCACTTTACATACATCCTCGGCAACAAAGACGATTGACCGTATT
ATAGATACTTTTTCTGCACAGGAAAAAGATAGTGTACGTGCCATGTTGGCAGAATCGCTACAAGCGGTTATCTCACAAAT
ATTATTAAAAAAACAACATCAAGGGCGTGTAGCTGCACATGAAATTATGATCGCCATCCCTGCAATCCGTAATTTAATTC
GAGAAAATAAAATTGCTCAAATGTATTCGGTATTACAAACGGGTGCCGTTTATGGCATGCAAACTTTAGATCAAAGTTTA
AAACATTTGTTACATCAAGGTACCATTACCTTACAGACTGCGCGTAGCCTTGCCAAGCAACCAGAGATGTTTAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

69.186

99.71

0.69
  pilT Acinetobacter baumannii D1279779

69.186

99.71

0.69
  pilT Acinetobacter baumannii strain A118

69.186

99.71

0.69
  pilT Acinetobacter baylyi ADP1

68.023

99.71

0.678
  pilT Pseudomonas stutzeri DSM 10701

64.244

99.71

0.641
  pilT Pseudomonas aeruginosa PAK

63.372

99.71

0.632
  pilT Legionella pneumophila strain Lp02

60.756

99.71

0.606
  pilT Legionella pneumophila strain ERS1305867

60.756

99.71

0.606
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.202

97.391

0.606
  pilT Vibrio cholerae strain A1552

62.202

97.391

0.606
  pilT Neisseria gonorrhoeae MS11

59.475

99.42

0.591
  pilT Neisseria meningitidis 8013

59.475

99.42

0.591
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.116

100

0.481
  pilU Acinetobacter baylyi ADP1

42.075

100

0.423
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.101

0.391
  pilU Vibrio cholerae strain A1552

39.701

97.101

0.386