Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QRD25_RS20300 Genome accession   NZ_CP127881
Coordinates   4264668..4265690 (-) Length   340 a.a.
NCBI ID   WP_286093596.1    Uniprot ID   -
Organism   Serratia marcescens strain ELP1.10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4259668..4270690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRD25_RS20270 (QRD25_20275) - 4260481..4260993 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  QRD25_RS20275 (QRD25_20280) endA 4261095..4261790 (+) 696 WP_015379043.1 deoxyribonuclease I -
  QRD25_RS20280 (QRD25_20285) rsmE 4261860..4262591 (+) 732 WP_004937458.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QRD25_RS20285 (QRD25_20290) gshB 4262602..4263552 (+) 951 WP_033632096.1 glutathione synthase -
  QRD25_RS20290 (QRD25_20295) - 4263686..4264249 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  QRD25_RS20295 (QRD25_20300) ruvX 4264249..4264671 (+) 423 WP_033632095.1 Holliday junction resolvase RuvX -
  QRD25_RS20300 (QRD25_20305) pilT 4264668..4265690 (-) 1023 WP_286093596.1 type IV pilus twitching motility protein PilT Machinery gene
  QRD25_RS20305 (QRD25_20310) - 4265711..4266418 (+) 708 WP_004937443.1 YggS family pyridoxal phosphate-dependent enzyme -
  QRD25_RS20310 (QRD25_20315) proC 4266438..4267259 (+) 822 WP_004937440.1 pyrroline-5-carboxylate reductase -
  QRD25_RS20315 (QRD25_20320) - 4267291..4267845 (+) 555 WP_004937436.1 YggT family protein -
  QRD25_RS20320 (QRD25_20325) yggU 4267842..4268132 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  QRD25_RS20325 (QRD25_20330) - 4268151..4268744 (+) 594 WP_049239920.1 XTP/dITP diphosphatase -
  QRD25_RS20330 (QRD25_20335) hemW 4268737..4269879 (+) 1143 WP_048796774.1 radical SAM family heme chaperone HemW -
  QRD25_RS20335 (QRD25_20340) - 4269916..4270350 (-) 435 WP_019456096.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36344.64 Da        Isoelectric Point: 7.2888

>NTDB_id=845068 QRD25_RS20300 WP_286093596.1 4264668..4265690(-) (pilT) [Serratia marcescens strain ELP1.10]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=845068 QRD25_RS20300 WP_286093596.1 4264668..4265690(-) (pilT) [Serratia marcescens strain ELP1.10]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCACAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCACTGCACAGGCCGAGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAGTCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGTGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAGGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGAGTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

48.795

97.647

0.476
  pilT Legionella pneumophila strain Lp02

48.795

97.647

0.476
  pilT Pseudomonas aeruginosa PAK

48.788

97.059

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.182

97.059

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.503

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365